- Research article
- Open Access
Exploratory behaviour in NO-dependent cyclase mutants of Drosophila shows defects in coincident neuronal signalling
BMC Neuroscience volume 8, Article number: 65 (2007)
Drosophila flies explore the environment very efficiently in order to colonize it. They explore collectively, not individually, so that when a few land on a food spot, they attract the others by signs. This behaviour leads to aggregation of individuals and optimizes the screening of mates and egg-laying on the most favourable food spots.
Flies perform cycles of exploration/aggregation depending on the resources of the environment. This behavioural ecology constitutes an excellent model for analyzing simultaneous processing of neurosensory information. We reasoned that the decision of flies to land somewhere in order to achieve aggregation is based on simultaneous integration of signals (visual, olfactory, acoustic) during their flight. On the basis of what flies do in nature, we designed laboratory tests to analyze the phenomenon of neuronal coincidence. We screened many mutants of genes involved in neuronal metabolism and the synaptic machinery.
Mutants of NO-dependent cyclase show a specifically-marked behaviour phenotype, but on the other hand they are associated with moderate biochemical defects. We show that these mutants present errors in integrative and/or coincident processing of signals, which are not reducible to the functions of the peripheral sensory cells.
We have shown that exploration in Drosophila is a powerful behavioural tool for analyzing the genetic basis of the machinery involved in the integration of multiple environmental signs . We therefore designed protocols to test the exploratory skills of the flies . We reasoned that the efficiency of exploration depends on the simultaneous integration of neurosensory signals during flight to guide the aggregation of individuals on a specific spot. When a food spot becomes disadvantageous, the flies reset a new cycle of exploration/aggregation . We previously showed that the first flies to find the appropriate food spot (explorers) signal to the others (followers) . Such collective exploration leads to remarkably efficient aggregation. They aggregate to screen sex partners and to lay eggs on the food spot most favourable for sustaining the development of larvae. This cooperative task suggests that costly individual foraging behaviour has not been selected by evolution because encounters would have been random, unpredictable and therefore unfavourable for mating. We assume in this report that "mistakes" in aggregation result from misguided integration of visual, olfactory and acoustic signals during flight and/or the overall exploratory process. We think that this simultaneous processing of neuronal signals can be analyzed experimentally by examining how flies achieve aggregation. These neuronal features include the detection and processing of a sequential and temporal order of signs generated in an unstable environment, to guide the flies' trajectory during flight and "decision making" to occupy a spot durably after landing. In other words, flies will be attracted by a bouquet of smells emitted by sources dispersed in the environment. Flies tend to land on an already-populated spot to maximize aggregation and to ignore "unoccupied" sources emitting the same bouquet. According to a "trade-off" mechanism, a high density population on a spot will prompt re-colonisation somewhere else by a fraction of the aggregated population. If a food spot is attractive because of the odours emitted but turns out to be distasteful and/or poisonous, the aggregate will probably not form. In this report, we designed tests derived from protocols described in our previous publication in order to analyze this stereotyped task . We show evidence that the capacity of the neuronal network to process coincidental signalling can be thoroughly evaluated in this way. Owing to the difficulties of encapsulating all the sensory functions and their integration in an entire body, we focus on the wing nerve, which conveys two major signals to the brain during flight: velocity- and chemo-detection. We used this neuronal system because the circuitry is accessible and the axons of at least two functionally distinct types of neurons (mechanoreceptors and chemoreceptors) are entangled, generating a unique bundle that projects to the same thoracic nervous structure site [2–5]. The two types of sensory information are therefore simultaneously transported via the same nerve. Although our comprehension of the integrative function of the brain in guiding the "decision" in an exploratory task is still incomplete, we report data suggesting that the collective exploration component is very robust; it is preserved in most of mutants tested (see the list of CNS mutants in Additional file 1) but drastically altered in sGC mutants. Moreover, NO (activator of sGC) has been reported to function as a combined 'slow-down and search signal' for growth cones of neuronal cells and a potent stop signal for developing neurites, which suggests mistakes in neuronal network and/or misguided neuronal wiring in flies lacking sGC (see arguments in discussion section). We reasoned that instable synapses and defective wiring should affect the synthesis/transport of synaptic vesicle components. Due to multiple difficulties to investigate whether synapses are appropriately formed at the right places in our mutants, we chose to examine indirectly the axonal transport of synaptic vesicle components in a very accessible nerve: the anterior wing margin nerve.
Strains used in this work
All the strains tested and not retained for further investigations are listed in Additional file 1. The references are indicated for those which are not publicly available. These lines were tested with the protocols described in figure 1. After five experiments showing little effect compared to control (C-S) we decided to stop investigating these mutants in the exploratory paradigm. dnc and rut and the GFP constructs P [GawB] elav [C155], P [UAS-syt.eGFP], w* (Bloomington Center, strain number 6923) and P [GawB]elav [C155], P [UAS-n-syb.eGFP], w*/FM7 (6923 and 6920) were obtained from the Bloomington Center. We focused on the sGC mutants due to a strong behavioural phenotype. Three mutants of sGC were used: GC782 and GC207 induced in the ca marked chromosome, and GC253 induced in the bv marked chromosome, have been published [6, 7]. The rescue is hsp-GC introduced in the GC207 background [6, 7]. The strains P [GawB] elav [C155], P [UAS-syt.eGFP], w* (Bloomington Center, strain number 6923 donator Kendal Broadie) and P [GawB]elav [C155], P [UAS-n-syb.eGFP], w*/FM7 (Bloomington Center, strain number 6920, donator Kendal Broadie) were crossed with sGC mutants as indicated below and in legends to figures.
The procedure has been described in a previous report . We used two systems, graphically represented in figures 1 and 2. Briefly, a pierced centrifuge tube is fixed upside down on a Petri dish so that grape juice food inside the assemblage is accessible only by passage through the holes. In the alternative system, such access is prevented by a layer of glass wool. Flies explore and colonize the two systems equally, and over a variable time course they leave the "wrong" system and flock on the "good" one. We described the kinetics of aggregation in a previous article , showing a sub-social component of aggregation. Briefly, our data argued in favour of the following behavioural sequence: flies walking on a food spot are moving objects that generate signs (wing motion, proboscis extension, acoustic signs) [8, 9]. Other flies land on the spot, attracted by those already aggregated, and avoid having to explore individually.
This test uses a chamber and three systems of pierced tubes fixed upside down on a Petri dish as described above. The tubes are painted black to avoid visual cues to guide the flies. Samples of grape juice (50 μl, 100 μl and 300 μl) are placed inside the three systems. The chamber is either saturated with grape juice odorants (hermetic sealing by plastic lid plus inaccessible source of grape juice inside as an odour generator) or not (the lid is a synthetic mattress that permits air exchange without the odour generator). After 2, 10 or 24 hours, the flies are counted in each system. This measures the ability of flies to explore in the absence of any odorant gradient or visual perception of conspecifics for guidance.
Taste aggregation test
This test uses a chamber and two food spots, as represented in figure 5. The two spots are identical grape juice sources, except that one is complemented with denatonium (1 mg/ml) . Denatonium confers bitterness (distasteful for flies as for humans, no perceptible odour) without interfering with the grape juice odorants. We observed a robust behavioural phenotype of aggregation on the source without denatonium for most strains from our shelves (this suggests that flies evaluate the source before they lay eggs on it). We determined the ratio of aggregated flies on the "wrong" spot versus the "good" one. We checked bitterness detection with quinine (1 mg/ml), which is less drastic than denatonium because, after tasting, denatonium blocks food uptake for a few minutes.
Sub social aggregation test
A complementary test measures the behaviour of a few flies when they face a choice between two food systems that are identical except that one is already aggregated with w1 flies (white-eyed flies can easily be discriminated from the red-eyed flies to be tested). The ratio of flies landing on the spot with pre-aggregated w1 flies versus the virgin spot measures the sub-social component of the strain being tested.
Physical lesion of the axonal bundle of the wing margin
The nerve of wings was sectioned with a razor in the proximal part of the wing of C-S under C02 anaesthesia. The flies (100) were tested one day after the surgery using a derived aggregation protocol (a chamber plus a food spot placed on the bottom).
Analysis of syt and n-syb synthesis in sGCmutants
We analyzed the effects of three sGC mutations on the levels of expression of syt.eGFP and n-syb.eGFP. We used stocks from the Bloomington Center that express the hybrid proteins syt.GFP or n-syb.GFP under the control of UAS/Gal4 (Gal4 is driven by the specific neuronal promoter elav) and located on the X chromosome. The progeny bearing the homozygous sGC mutation (associated with recessive markers ca and bv on the third chromosome) and fluorescence (one copy of the transgene elav-Gal4-UAS syt.GFP or n-syb.GFP on the first chromosome) were analyzed. Double heterozygous hsp-sGC/(;)P [GawB] elav [C155], P [UAS-syt.eGFP], w* and hsp-sGC/(;) P [GawB] elav [C155], P [UAS-n-syb.eGFP], w* were heat-shocked (day 3). Fluorescence in the soluble extract was measured two days later (day 5). Controls were heterozygous flies, +/"GFP". Similarly, we measured the fluorescence intensity in the doubly heterozygous n-syb.GFP/rut or dnc and syt.GFP/rut or dnc. rut codes for a cyclase isoform and dnc for a phosphodiesterase (both are on the X chromosome), so that the cAMP levels in the two strains are markedly different, although learning and memory behaviour showed identical defects [10, 11]. Only females of these strains were considered for analysis. The strains P [GawB] elav [C155], P [UAS-syt.eGFP], w* (Bloomington Center, strain number 6923 donator Kendal Broadie) and P [GawB]elav [C155], P [UAS-n-syb.eGFP], w*/FM7 (Bloomington Center, strain number 6920, donator Kendal Broadie) were crossed with sGC mutants. Fies (F3, F4) showing ca and bv along with fluorescence were selected. Strain 6920 is homozygous lethal, so strain GFP; sGC, homozygous for the mutation, is heterozygous for fluorescence. Strain 6923 is homozygous viable, so in this case we used GFP;sGC males in the indicated experiments. One hundred fly heads were ground in a glass Potter and the extract (500 μl) was centrifuged (10000 rpm for 1 h at 4°C). Fluorescence measurements were carried out on the supernatant using a 90 well plate with 100 μl of the extract in each well. The GFP domain is attached to the C-terminal of syt so the fluorescence is expressed on the cytosolic side of the vesicle membrane (syt has its N terminal in the vesicle lumen), while the n-syb-GFP construct expresses fluorescence inside the vesicle (syb has its C terminal in the vesicle lumen and its N terminal on the cytosolic side). In this latter case, the supernatant of the extract was briefly sonicated before fluorescence was measured.
We used no chemical reagent for protein dosage (such as Biorad) of the crude head extract because eye pigmentation interferes. We started with equal number of heads and we verified protein concentrations as follows: a densitometric analysis of a few typical bands was performed after acrylamide gel electrophoresis, colloidal Coomassie blue staining and a 10% acetic acid/25% methanol wash for 24 hours. The concentrations were normalized with a control value (C-S fly) as reference. The protein contents of the samples differed by less than 10%.
Immunoprecipitation analysis of syt in sGCmutants
One hundred heads were cut off with a razor (flies are immobilized by a brief pulse of CO2) and ground in a glass Potter in 500 μl 20 mM phosphate buffer, pH = 7, plus 1 μg/ml PMSF. This extract was centrifuged 10000 rpm at 4°C for 1 hour and the supernatant was incubated with anti-GFP agarose. The pull-down material was then solubilized with 0.5% nonionic detergent (Tween 20 1%(Sigma Aldrich) in 20 mM phosphate buffer, pH 7, plus 1 μg/ml PMSF). After brief centrifugation, the supernatant was submitted to immunoprecipitation using anti syt plus anti rabbit agarose (I hour incubation with anti syt, then with anti rabbit agarose under constant agitation for 1 hour in 20 mM phosphate buffer, pH 7, plus 1 μg/ml PMSF). The pellet was then washed twice in buffer without detergent and submitted to gel electrophoresis analysis.
Determination of kinase activity
The kinase activity was assayed as described elsewhere using 32 γP-ATP and histone IIb as substrate [12, 13]. Briefly the amount of radioactive phosphate incorporated in histone IIb is measured using a beta counter.
Analysis of syt-GFP and n-syb-GFP in the axonal bundle of the wing margin in sGCmutants
We analyzed the fluorescence of the hybrid molecules (syt-GFP and n-syb-GFP), placed in a sGC genetic background as described above (see analysis of syt and n-syb synthesis), in the wing margin axonal bundle. Wing of flies were examined at day 5. The rescue hsp-GC in GC207ca background was analyzed as double heterozygous (hsp-GC plus syt.GFP or syb.GFP). For these strains, flies were heat-shocked at day 3 and analyzed two days later.
Performance in exploration is altered in sGCmutants
In a previous paper we reported that searching behaviour involves a strong collective component and flies help each other through various signs to optimize the efficiency of exploration . In other words, we showed that individual flies tested one by one were distributed randomly in a chamber, very different from the distribution obtained with a population of flies. Individual flies were counted in three compartments: immobile on surfaces, flying in the chamber, inside a pierced tube with food. We observed no evolution of the frequencies with which flies were present in these three compartments over a 5-hour time course. Furthermore, these frequencies were to some extent similar in all the strains tested including the sGC mutants (see Additional file 2 and the following paragraphs). We then assessed the collective ability of the flies to find a hidden food source in a misleading environmental context. Briefly, two systems compete for the attention of the flies in a chamber. One system consists of a pierced tube; the flies have to pass through the holes to reach a grape juice reward placed inside. The other system is the same except that it includes a layer of glass wool to prevent access. Controls show that the ability of sGC mutants to aggregate was unaffected (figure 1). We analyzed the kinetics of distribution and found that most mutants and C-S (wild type) flies, along with control ca or bv flies, stay in the unfavourable tube for only a limited time. When the experiment is carried out with sGC mutants, equal numbers of flies remain for longer in both tubes (see figure 2). These mutants are trapped in the "forbidden" system by the odour gradient and it is obviously difficult for them to leave it and find the nearby "good" system. The other strains entering this system leave quickly and colonize the "good" system. In the same context, we found that heat shock-induced expression of sGC during metamorphosis (single acute heat shock delivered at the late third instar stage), then during the adult age (single acute heat shock delivered to 3 day old flies), significantly restored the wild type phenotype (see figure 2). The complexity of the tasks performed (directional flight, detection of gradient, finding the holes) by most of the strains tested, including the sGC mutants, eliminates major sensory defects in these flies (visual, olfactory, acoustic). Sensory cells are not likely to account for the flaws in searching observed in the sGC mutants.
sGCmutants disconnect exploration from detection of odour
Drosophila have highly efficient machinery for exploring the environment, and in the course of exploration they simultaneously integrate olfactory, acoustic and visual signals to guide their journey. We used an odour-saturated atmosphere to evaluate how odour gradients guide exploratory behaviour in mutants. We first carried out experiments to analyze aggregation in an atmosphere aerated to preserve the odour gradient emitted from a source of grape juice (figure 3). A parallel protocol was performed in a chamber with a grape juice-saturated atmosphere that eliminated gradients (see figure 4). The final aggregates were counted in each tube. As expected, the most favourable tube (more grape juice) attracted the larger aggregates in the aerated atmosphere (figure 3). When the experiment was carried out with the gradient abolished, we observed that most mutants tested, and the wild type fly (Cantonese S.), lost their exploratory behaviour and were unable to find the food sources. In odour-saturated air, the sGC mutants were significantly more capable of finding the hidden source without environmental olfactory cues for guidance (figure 4). Remarkably, the behaviour of the sGC mutants suggests that their exploratory capacity is not eliminated by the absence of gradient, as we might expect, so it is partially independent of olfaction. The rescue (third instar larvae then 3 day old adults exposed to a single acute heat shock) restores wild type behaviour (figure 4). Our data might be consistent with more aggressive and disorganized exploratory behaviour and might accord with published accounts of the behaviour of sGC mutant larvae, showing hyper-locomotion . However, we found that the most favourable spot was systematically more populated with sGC mutants in the saturated atmosphere, which suggests that unguided and random visiting of sites does not exclude secondary sub-social aggregation. We therefore conclude that sGC mutants disconnect exploration from the detection of odour gradients. We think that the protocol includes its own internal controls for the minimal functionality of the peripheral sensory systems. These data suggest regulation/control of exploration via the NO/cGMP pathway.
sGCmutants disconnect taste and aggregation behaviour
Drosophila aggregate on food spots; the evolutionary advantage of this is that females in aggregates can screen more males for mating. Furthermore, females have to assess the quality of food resources in order to lay eggs. Interestingly, the choice of aggregation spot is a reliable collective evaluation of the food resources necessary to sustain larval development [14, 15]. Therefore, we reasoned that aggregation on a misleading and unfavourable spot in competition with a favourable spot could highlight a collective "mistake" in performing an aggregation task. We designed an experimental protocol as follows: two spots of identical grape juice food were placed in a chamber and constituted alternative choices for the attention of the flies. The only difference was that one was complemented with denatonium, which confers a bitter taste. The control showed that the total aggregated versus free in the chamber was of the same order in the different strains tested (figure 5). We checked then that the bitter compounds are repulsive, and observation showed that recognition of bitter molecules is little affected by the mutations tested, including sGC (figure 6). Using this protocol, we found that the sGC mutants aggregated significantly on the bitter spot during the time course of the experiment, whereas the controls flocked on the favourable spot (see figure 6). The rescue (double heat shock delivered to third instar then 3 day old adult) significantly corrected the phenotype (figure 6). The control showed that the total aggregated versus free in the chamber was of the same order in the different strains tested, which again supports the inference that sGC mutations induced flaws in coincidental neuronal signalling. This suggests that sGC mutants partly disconnect taste from aggregation.
sGCmutants show sub-social aggregation defects
We also tested our mutants using a variant experimental design. We used the same two spots of grape juice food in a chamber except that one was already aggregated with w1 flies. In other words, we tested the behaviour of our mutants in the presence of pre-aggregated w1 flies. This strain is recognizable by the white eye colour, and we reported in a previous article that their altered vision triggers long-lasting and stable aggregation . sGC mutants (GC207 and GC782) showed no significant preference between the aggregated spot and a virgin fresh alternative. In this test, other stocks tested and the control fly ca showed a tendency to flock on the already-aggregated spot (see figure 7). The controls showed that the ratio of total flies on food spots to flies free in the chamber was of the same order in all the strains tested (figure 7), and this again allows us to eliminate major peripheral sensory defects (olfactory or visual). This experiment shows that sGC mutants have an altered sub-social component for guiding exploration (see ref. 1 for arguments in favour of cooperative searching in Drosophila).
Analysis of neural circuitry of coincident signalling involved in exploration/aggregation behaviour
We then tried to identify the cells involved in these behaviours. We failed to uncover the relevant circuitry because sGC is ubiquitous in the brain. Immunohistology using an anti-sGC antibody showed no specific labelling in any anatomical part of the brain (data not shown). sGC mutants have no structural brain defect as far we could observe by microscopy (data not shown). The cellular mass was identical to wild type, as measured by quantification of a neuronal marker recognized by anti-HRP antibody (data not shown). To approach the molecular mechanisms involved in these behavioural defects, we performed biochemical experiments with adult brains of sGC mutants and the rescue (induction of hsp-sGC at adult age in mutant background). We pursued this work although the neurons involved in the coincidence systems are probably "diluted" in the CNS. We analyzed the effects of three sGC mutations on the levels of expression of syt.eGFP and n-syb.eGFP. We found that sGC mutations slightly increase the levels of fluorescence of the two hybrid molecules, although the global effect is modest. Moreover, the rescue (induction of hsp-sGC at adult age in mutant background) diminishes significantly the level of fluorescence of the two hybrid molecules (see Additional file 3). We didn't see differences in sGC mutants and control regarding the amount of the three isoforms of syt molecules (see Additional file 4). However two syt isoforms were down regulated after overexpression of sGC in the rescue after heat shock. This argues in favour of a role for sGC to regulate the synthesis and/or transport of synaptic vesicle components along the axons after wiring.
In view of this complexity, we decided instead to focus on a simple coincidence system, the sensory organs of the wing margin, which has the advantage that two types of sensory neurons are entangled in the same axonal bundle: mechanoreceptors and chemoreceptors [2–5]. This simple system is highly accessible and relevant to flight guidance. Unilateral lesion of the wing margin nerve of C-S abolishes the aggregation phenomenon and the collective behaviour of these flies resembles that of individual flies. We deduced that the integration of signals generated by sensory cells in the wing during flight contributes to the fly's synchronized "decisions" (see figure 8).
Moreover, we reasoned that specific mechanism(s) and molecular machinery involving sGC might influence the mutual interaction of the two types of neuron in order to achieve efficient exploration. We therefore analyzed the fluorescence of hybrid molecules expressed in synaptic vesicles in the wing margin axonal bundle (syt-GFP and n-syb-GFP) placed in a sGC genetic background. The results, reported in figure 9, show evidence for a role of the NO/cGMP pathway in this neuronal system. We observed that syt-GFP was up-regulated in these types of neurons only in sGC mutants whereas the overall fluorescence in the brain was little changed as reported above.
Many reports have described the genetic complexity underpinning foraging behaviour, especially in Drosophila [16–18], and an abundant literature reports the successful application of genetic tools in deconstructing complex behaviours in model animals [19–21]. Complex behaviours involve brain integration of multiple environmental signs in order to guide decisions. Flies might disregard some signs and overreact to others depending on the context. In this sense, the paradigm of molecular sensors of coincidence is advantageous in linking molecules to complex behaviour . We carried out this work because the well-documented foraging genes in Drosophila and honeybee code for cGMP-dependent kinases, and pleiotropic effects are induced downstream of their activation [12, 13, 23]. By better integrating the multiple environmental signs sensed by the neurosensory systems (visual, acoustic, olfactory, tactile), explorers variants (honeybee, drosophila) should be more equipped to face the adversity and hostility of the environment. In this report, we used tests designed to evaluate whether certain mutations in key molecules can specifically affect the integration of multiple neuronal signals that contribute to enabling flies to perform stereotyped tasks in exploration. We considered that flies naturally (innately) perform tasks involving skills and strategies for finding food and sexual partners, otherwise they would wander in the environment. The stereotyped tasks forming the basis of our tests constitute cycles of exploration/aggregation. Although highly complex gene networks and pleiotropy account for these complex behaviours, the phenomenon of coincidence highlighted in our tests seems to relate to a more restricted subset of genes. We screened more than twenty mutants of genes involved in neuronal signalling (Cam kinase, PKC, PKG mutants, mutants of synaptic machinery; see the list in Additional file 1) and found that mutants of sGC (along with dnc mutants) present a marked behavioural phenotype. Abundant literature documents the role of sGC in neurobiology. Nitric oxide (NO) diffuses as short-lived messenger through the plasma membrane and serves, among many other functions, as an activator of the soluble guanylyl cyclase (sGC) [24, 27]. Authors have reported that NO could affect the steering decisions of growth cone during neuronal pathfinding . Furthermore, authors have described that presynaptic elements are assembled into 'prototerminals' before being transported down the axons and these structures stop their migration when the axons contact a dentrite . Interestingly direct application of a NO donor to neurons resulted in inhibition of axonal transport of synaptophysin and synaptotagmin I tagged with EGFP in neuron culture . Authors reported also that sGC is asymmetrically localized to the developing apical dendrites in mammal neurons and is required for the chemoattractive effect of some molecules . Authors have reported also a role of NO as "stop and go" signal for neuronal processes [32, 33], a role in communication between sensory afferent neurons , in long term depression [35, 36] and in retrograde signal to regulate the synaptic vesicle recycling [37–39]. All together these elements argue for the presence of fragile and/or misguided synapses in our sGC mutants. The wing nerve analysis allowed us to evaluate that sGC mutations result in a continued synthesis and/or transport of synaptic vesicle components, which should be stopped by proper axon/dentrite wiring. The sGC mutants are viable. sGC is therefore not essential gene for major developmental steps. However, we were able to see obvious effect of this gene on the neuronal coincidence signalling based on behavioural tests. Our behavioural data supports the conclusions of a deficiency of integration of signals emanating from neurosensorial systems.
As distinct from learning and memory, our paradigm mostly concerns synchronized fly "decision making" resulting from the simultaneous processing of environmental signs. We might suggest by deduction that sGC mutants appear to unravel the function of this gene in neurons involved in integrating and synchronizating cellular signals. On the other hand, the adult wing of Drosophila, equipped with sensory bristles which are mechanoreceptors and chemoreceptors, is highly accessible. This constitutes a simple coincidence system. Although biochemical analysis revealed no detectable major defects in the brains of sGC mutants, the wing nerve shows altered regulation of molecular components, which might account for the exploratory disorders.
- sGC :
soluble guanylate cyclase
- hsp :
heat shock promoter
- rut :
- dnc :
- syt.eGFP :
synaptotagmin/green fluorescent protein fusion
- n-syb.eGFP :
synaptobrevin/green fluorescent protein fusion
- NO :
- NOS :
Nitric oxide synthase
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We thank PA Sallin and JF Ferveur for helpful comments and discussions. We thank M Sokolowski and G Criedl for collaborative work on the sGC mutants. We thank RJ Greenspan for advice. We thank Dr. Zuker, A. Becker and RW Hardy for generously providing mutants and transgenic flies through initial collaboration with Dr. Sokolowski. We thank H. Bellen for providing polyclonal anti-synaptotagmin and R. Kelly for anti-synaptobrevin. This work was supported by CNRS (Centre National de Recherche Scientifique) and a grant from INRA (department of "santé des plantes et environnement").
ST and AR did the behavioural experimental work. AG did biochemistry work (specially phosphorylation and immnunoprecipitation). GE did the fluorescence analysis AR did the experimental designs of experiments and ST contributed through discussion and data analysis.
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Tinette, S., Zhang, L., Garnier, A. et al. Exploratory behaviour in NO-dependent cyclase mutants of Drosophila shows defects in coincident neuronal signalling. BMC Neurosci 8, 65 (2007). https://doi.org/10.1186/1471-2202-8-65
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