GC mutants show sub-social aggregation defects. (top) Social aggregation of two mutants GC207 and GC782. Pre-aggregated w1 flies (50 flies ± 10) were used to "lure" mutants. Mutants (20 flies, 5 days old, red eyed, females to avoid pheromone interference) were released in the chamber without disturbing the aggregated spot (w1 aggregates stable, probably because of altered vision). Simultaneously, a second fresh spot was placed inside. Here, the graph represents the time course of the ratio of aggregated flies (GC 207 or GC 782) on the pre-aggregated spot versus the fresh one. Values are mean ± SEM (n = 10). The GC207 mutant against control ca gave T value: 3.38, P = 0.003 and degrees of freedom: 18. Heat shock rescue was carried out as in figure 1. (bottom) The control of flies aggregated versus the total in the chamber is represented for the strains tested above. We saw no significant differences between the strains. The apparatus was the same than in the figure 5 and 6.