Synthesis/transport of synaptic vesicle in wing nerve of sGC mutants. The strains P [GawB] elav [C155], P [UAS-syt.eGFP], w* and P [GawB] elav [C155], P [UAS-n-syb.eGFP], w*/FM7 were crossed with sGC mutants (see materials and methods section). Flies bearing one copy of hybrid GFP (first chromosome) and homozygous for sGC mutations were analyzed for the fluorescence in the wing. The rescue hsp-GC in GC207ca background was analyzed as double heterozygous (hsp-GC plus syt.GFP or syb.GFP). Flies were heat-shocked at day 3 and analyzed two days later. Positive control: MARCM system (chromosome recombination based on flipase under heat-shock promotor induces axonal GFP marker -Bloomington Center stock 5144 and 5134).