Analysis of a simple coincidence system in sGC mutants: the axonal bundle in the wing margin . (top) The nerve was sectioned with a razor in the proximal part of the left wing of C-S under C02 anaesthesia. The flies (100) were tested one day after the surgery using a derived aggregation protocol (a chamber plus a food spot placed on the bottom). As controls, we report a comparative assay with the w1 mutant (altered vision), Cy (impaired flight) along with sGC mutant (GC 207) and its rescue (after heat shock). Ten experiments were carried out. The graphs represent the number of experiments showing aggregation above 50% of the total after 5 hours. (bottom) A comparative test of lethality with 50 flies of the indicated strains (8 days old flies) and the nerve sectioned strain carried out 5 days after the surgery.