- Research article
- Open Access
Human neuronal stargazin-like proteins, γ2, γ3 and γ4; an investigation of their specific localization in human brain and their influence on CaV2.1 voltage-dependent calcium channels expressed in Xenopus oocytes.
BMC Neurosciencevolume 4, Article number: 23 (2003)
Stargazin (γ2) and the closely related γ3, and γ4 transmembrane proteins are part of a family of proteins that may act as both neuronal voltage-dependent calcium channel (VDCC) γ subunits and transmembrane α-amino-3-hydroxy-5-methyl-4-isoxazoleproponinc (AMPA) receptor regulatory proteins (TARPs). In this investigation, we examined the distribution patterns of the stargazin-like proteins γ2, γ3, and γ4 in the human central nervous system (CNS). In addition, we investigated whether human γ2 or γ4 could modulate the electrophysiological properties of a neuronal VDCC complex transiently expressed in Xenopus oocytes.
The mRNA encoding human γ2 is highly expressed in cerebellum, cerebral cortex, hippocampus and thalamus, whereas γ3 is abundant in cerebral cortex and amygdala and γ4 in the basal ganglia. Immunohistochemical analysis of the cerebellum determined that both γ2 and γ4 are present in the molecular layer, particularly in Purkinje cell bodies and dendrites, but have an inverse expression pattern to one another in the dentate cerebellar nucleus. They are also detected in the interneurons of the granule cell layer though only γ2 is clearly detected in granule cells. The hippocampus stains for γ2 and γ4 throughout the layers of the every CA region and the dentate gyrus, whilst γ3 appears to be localized particularly to the pyramidal and granule cell bodies. When co-expressed in Xenopus oocytes with a CaV2.1/β4 VDCC complex, either in the absence or presence of an α2δ2 subunit, neither γ2 nor γ4 significantly modulated the VDCC peak current amplitude, voltage-dependence of activation or voltage-dependence of steady-state inactivation.
The human γ2, γ3 and γ4 stargazin-like proteins are detected only in the CNS and display differential distributions among brain regions and several cell types in found in the cerebellum and hippocampus. These distribution patterns closely resemble those reported by other laboratories for the rodent orthologues of each protein. Whilst the fact that neither γ2 nor γ4 modulated the properties of a VDCC complex with which they could associate in vivo in Purkinje cells adds weight to the hypothesis that the principal role of these proteins is not as auxiliary subunits of VDCCs, it does not exclude the possibility that they play another role in VDCC function.
The mutation underlying the absence epilepsy phenotype of the allelic stargazer (stg) and waggler (wag) mutant mice occurs in a gene, cacng2, whose product, stargazin, has been hypothesized to be a neuronal voltage dependent calcium channel (VDCC) γ subunit . VDCCs are intrinsically involved in the regulation of a multiplicity of Ca2+ dependent processes in many different cell types where they are inserted into the plasma membrane as hetero-oligomeric complexes of a pore-forming α1 subunit with auxiliary β, α2δ and possibly γ subunits .
The first VDCC γ subunit to be identified (γ1) [3–5] was found to be solely expressed in skeletal muscle, where its function is to limit calcium entry through the L-type VDCCs of skeletal myotubes [6, 7]. VDCCs purified from neuronal tissues did not appear to possess a γ subunit [8–11]. However, despite sharing only weak protein sequence identity with γ1 (25%), stargazin (γ2) was suggested to represent the first example of a neuronal VDCC γ subunit based on its similar tetra-spanning transmembrane structure to the γ1 subunit and its ability to weakly modulate VDCC-current properties in vitro . Subsequent investigations have identified six other stargazin-like genes which are currently classified as cacng3-cacng8 (encoding proteins γ3 - γ8), in a continuation of the VDCC γ subunit nomenclature [12–17].
Investigation of the functional influence of these stargazin-like γ proteins upon VDCCs has yielded mixed results. Some laboratories have reported that γ2 and its close homologue γ4 cause small hyperpolarizing shifts in the voltage dependence of steady-state inactivation [1, 14, 18]. This however, might be dependent upon which other auxiliary subunits are co-expressed in the VDCC complex under investigation . In contrast, Chen et al.  showed whole cell VDCC currents from the cerebellar granule cells of stg mice, which effectively lack the γ2 subunit, do not have significantly altered voltage-dependence of activation or inactivation compared to wild type. Other laboratories reported that γ2 or γ3 can significantly reduce peak current amplitudes of N-type VDCCs expressed in Xenopus oocytes, but only when co-expressed with an α2δ1 subunit , and supporting this, that thalamic relay neurons from stg mice express enhanced low and high voltage-activated VDCC currents compared to wild type . Furthermore, clear biochemical evidence has been generated for a direct interaction of γ2 with the VDCC CaV2.2 α1 subunit [20, 22]. Another stargazin-like protein, γ7, which is phylogenetically distinct from γ2, γ3 and γ4 [13, 15], almost abolishes the expression of CaV2.2 when co-expressed in vitro, and also reduces Ca2+ currents via CaV1.2 and CaV2.1 channels . However, our data indicated that the influence of γ7 on VDCC function is to reduce α1 subunit protein expression, a functional property unlike anything reported for the other stargazin-like proteins, which suggests that γ7 is not a subunit of a calcium channel complex .
Whilst controversy surrounds the role of the γ2, γ3 and γ4 stargazin-like proteins in relation to VDCC modulation, a clear function has been determined for these proteins as chaperones for the appropriate trafficking and receptor biogenesis of alpha-amino-3-hydroxy-5-methylisoxazole-4-propionic acid (AMPA) receptors [19, 23–25]. Consequently, these three proteins together with their close homologue γ8, were recently dubbed transmembrane AMPA receptor regulatory proteins (TARPs) . A primary interaction (probably via transmembrane and/or extracellular regions ) promotes the trafficking of the GluR subunits to the plasma membrane, and a secondary interaction of the C-terminus of stargazin with PSD-95 or a similar cytoskeletal protein via a PSD-95/DLG/ZO-1(PDZ)-binding motif facilitates the lateral relocation of the glutamate receptor complex to its correct position in the post-synaptic density  and hence influences the number of AMPA receptors located at this site .
Elucidation of the differential distribution of the stargazin-like proteins coupled with studies of the physiological abnormalities underlying the epilepsy phenotypes of the mice expressing what are effectively null mutations of γ2 has also helped to determine some of the normal functions of these proteins in the murine CNS. The stg and wag mice display a loss of the fast component of EPSC at mossy fiber to cerebellar granule cell synapses [28, 29], plus reduced synaptic transmission at parallel fiber Purkinje cell synapses in wag . However, the synaptic transmission to CA1 pyramidal cells (Schaffer collateral projection) in stg is not altered . In situ hybridization studies have determined that murine γ2 is normally expressed at its highest levels in the cerebellum [1, 14, 19]. γ3 and γ4 mRNAs are also detected in mouse cerebellum but γ3 mRNA has been detected only in the Golgi neurons of the granule cell layer and is absent from the molecular layer . γ4 mRNA is localized to the Purkinje, rather than the granule cell layer . Whilst this manuscript was in preparation, Tomita et al.  reported that γ2 is the only TARP expressed in rat cerebellar granule cells, but like mouse, all isoforms are detected in hippocampus [1, 14, 19, 25]. The genetic defect in stg results in the loss of γ2 mRNA and protein and does not appear to result in up- or down-regulated expression of γ3 or γ4 [1, 22]. Collectively, these data suggest that stg mice exhibit the loss of fast synaptic transmission in the mossy fiber to granule cell synapse because γ2 is the major and possibly the only stargazin-like protein in the cerebellar granule cells of wild-type mice and no other stargazin-like protein is expressed at sufficient levels to rescue normal AMPA receptor trafficking and maturation. The reason that synaptic transmission to CA1 pyramidal cells in stg mice is preserved is probably because, although γ2 is not expressed in stg, the total remaining TARP expression levels in hippocampal CA1 pyramidal cells are adequate to promote normal surface expression of mature AMPA receptors at the postsynaptic membrane in this synapse.
It was therefore of great interest to examine whether the expression patterns of the human stargazin-like proteins paralleled those of mouse and if they were differentially expressed in the various cell types of each tissue. This study presents the differential distribution of γ2, γ3 and γ4 in human brain by northern blotting and more detailed immunohistochemical analysis of their expression in human cerebellum and hippocampus. In addition, we used the results of our distribution study to investigate whether the human γ2 and γ4 could modulate currents gated by a VDCC complex heterologously expressed in Xenopus oocytes that was assembled from the major VDCC subunits expressed in a cerebellar Purkinje cell.
Northern Blot analysis of mRNA distribution
The overall tissue distribution of human γ2, γ3 and γ4 mRNAs was analyzed by northern blotting. Specific cDNA probes representing each gene were generated as described in the methods and hybridized against a human multiple-tissue northern blot (Figure 1A) and two brain region blots (Figure 1B and 1C). The γ2-specific cDNA probe detected two mRNAs of approximately 7 kb and 3 kb. Bands of similar size were reported in a mouse multiple-tissue blot probed with a murine γ2 cDNA probe . Like mouse, the human γ2 mRNA transcripts were detected only in brain (Figure 1A), and were particularly abundant in cerebellum, cerebral cortex, occipital lobe, frontal lobe and temporal lobe, hippocampus and thalamus (Figure 1B and 1C). γ2 transcripts were detected at somewhat lower levels in medulla, putamen, amygdala and substantia nigra but only weakly in caudate nucleus. γ2 transcripts were absent from corpus callosum and the sub-thalamic nucleus and spinal cord.
A γ3 cDNA probe detected one 2.0–2.1 kb γ3 mRNA transcript which like γ2, was exclusively localized to the brain (Figure 1A). However, γ3 mRNA was detected only in cerebral cortex, including occipital lobe, frontal lobe, and temporal lobe, the putamen, caudate nucleus, amygdala and hippocampus and was absent from all of the other regions probed (Figure 1B and 1C).
The γ4-specific probe identified an mRNA of approximately 4 kb detected exclusively in brain (Figure 1A). This was widely detected throughout the brain but was most prevalent in the putamen and caudate nucleus. Unlike γ2 or γ3, γ4 mRNA was also weakly detected in spinal cord.
Generation and characterization of γ specific antisera
In order to investigate the distribution of the γ2, γ3 and γ4 proteins and to analyze their expression in different neuronal cell types we generated specific antisera against each isoform. These were generated using peptide immunogens that were selected to be subtype-specific as determined by multiple sequence alignment. To confirm activity against the corresponding holopeptide and lack of cross-reactivity with the other subtypes, each of these antisera were validated against the different subunit proteins recombinantly expressed in COS-7 cells. Cells were transiently transfected with a pMT2 expression vector containing either the human γ2, γ3 or γ4 cDNAs, and 2–3 days later were analyzed by immunocytochemistry. All three γ isoforms could be readily detected and were localized largely in the plasma membrane of the cells (Figure 2A). The specificity of the antibodies (Abs) was confirmed by incubating non-transfected cells with each primary Ab, which in each case gave no immunoreactivity (Figure 2B). Similar results were obtained when cells expressing one of the γ isoforms were incubated with Abs directed against either of the other two γ proteins (data not shown). Cells transfected with a γ cDNA but which were not permeablized after fixation also exhibited no immunoreactivity (Figure 2C). Since the peptide sequences chosen for immunization are in the C-terminus, these results indicate this portion of recombinantly expressed γ2, γ3 and γ4 is located intracellularly. Co-transfection of VDCC subunits CaV2.1 and β4 did not alter the expression pattern of the γ proteins indicating that there are no effects on trafficking and that the anti-γ Abs did not cross-react with the CaV2.1 or β4 subunits (data not shown).
Immunolocalization of γ2, γ3 and γ4in human cerebellum and hippocampus
Immunohistochemical analysis was carried out to examine the differential expression of γ2, γ3 and γ4 in human cerebellum and hippocampus. Control experiments to measure non-specific binding to tissue sections were performed by pre-absorbing antisera overnight with 100 μM of the relevant peptide (Figures 3E and 3I, 4C and 4D, 5C and 5D and 6B) and by omitting the primary antibody (data not shown).
Figure 3 shows the pattern of staining observed in the cerebellum using γ2, γ3 and γ4 specific antisera. Moderate γ2-specific staining was seen in the molecular layer suggesting expression in the dendrites of cerebellar Purkinje neurons (Figure 3A). Small cell bodies in the molecular layer that were most likely stellate or basket cell interneurons also stained positively. The γ2 immunostaining was also strong in the soma of Purkinje neurons extending to the dendrites, but decreasing in intensity following the first few bifurcations (Figure 3A). Cerebellar granule cells were moderately stained for γ2 (Figure 3B), although assessment of immunostaining in this cell type was difficult since the majority of cell volume is comprised of the nucleus. The strongest γ2 immunostaining in this region was actually in the interneurons. Figure 3C shows strong γ2 immunostaining in the cell bodies of the dentate cerebellar nucleus with only weak to moderate staining in the surrounding neuropil.
Very weak γ3 staining was observed in Purkinje cell bodies and in the interneurons of the granule cell layer (Figure 3D). Staining of the molecular layer neuropil and the granule cells is comparable to the peptide pre-absorption control (Figure 3E). It is therefore possible that the γ3 protein is poorly represented in these particular cell types and not completely absent from the cerebellum. No γ3 immunoreactivity was observed in the dentate cerebellar nucleus (data not shown).
Staining of a human cerebellar folium for γ4 is shown in Figure 3F. The molecular layer and adjacent granule cell layer was lightly to moderately stained, but the staining of the Purkinje cell bodies and dendrites was striking. γ4 immunostaining extended well into the Purkinje cell dendritic arbors from the cell body indicating that γ4 expressed well throughout this cell type. The cell bodies that stained positively in the molecular layer were small interneurons. In the granule cell layer the interneurons stained strongly for γ4 whereas the granule cells appear unstained (Figure 3G). In the dentate cerebellar nucleus (Figure 3H), γ4 immunostaining was detected strongly in the perisomatic neuropil with only weak staining in the cell bodies. Interestingly, this staining pattern was almost the complete inverse of the γ2 immunostaining in the same region (Figure 3C).
Hippocampus and dentate gyrus
Immunohistochemistry revealed differential expression patterns for γ2, γ3 and γ4 in the different regions and cell types of human hippocampus and dentate gyrus (Figure 4, 5 and 6). When incubated with the anti-γ2 Ab, generalized staining of the cell layers and neuropil throughout the hippocampal formation was observed. In Figure 4A the alveus, stratum oriens most adjacent to the CA1 pyramidal layer, and the pyramidal layer all stained moderately for γ2. The stratum radiatum and lacunosum-moleculare stained much more weakly. The CA2 and CA3 pyramidal layers stain well for the γ2 subunit (Figure 4B) and the intensity of staining in the alveus increased slightly in this region. It is however apparent that there is only a very weak staining of the strata oriens and radiatum in the CA2/3 regions; yet, moderate immunostaining is detected in the stratum lucidum. Weak staining is visible in the stratum lacunosum-moleculare. In the same section we also observed moderate γ2 staining in the molecular layer of the dentate gyrus and strong immunostaining in the granule cells (examined at higher magnification on Figure 4H). The γ2 staining was weak or absent in the polymorphic layer, but distinct fibers were stained which course across this region. Moderate staining of cell bodies in CA4 was also observed (Figure 4B, top left portion of the panel). Pre-absorption controls using the γ2 peptide immunogen (Figures 4C and 4D) show the background staining in a serial section of the same hippocampus as in Figures 4A and 4B. The perisomatic staining in the stratum pyramidale, in the region of the CA1 towards the CA2 was moderate, with slightly more intense somatic staining (Figure 4E). An apparently higher level of γ2 staining in the CA2 and CA3 regions (Figures 4F and 4G) compared to CA1 was probably due to a higher density of pyramidal cell soma in these regions compared to the CA1 region .
Staining for γ3 was absent from the alveus, stratum oriens, stratum radiatum and lacunosum-moleculare, but the pyramidal layer of the CA1, CA2 and CA3 regions of Ammon's horn strained strongly, with lesser immunostaining in the subiculum and CA4 regions (Figure 5A and 5B). Closer examination revealed that the pyramidal cell bodies stained strongly for γ3 and this staining was strongest in CA3 and became progressively weaker through the CA2 and CA1 regions and into the subiculum. High power images of CA1 (Figure 5E), CA3 (Figure 5F), and subiculum (Figure 5G) determined that this was most likely to be caused by increased cell densities in the CA2/3 regions rather than more intense staining of pyramidal neurons, although perisomatic staining was more intense in the CA3 region than CA1, and absent from the subiculum. Visible in Figure 5A and 5B, and also observed at an increased magnification in Figure 5H, the granule layer of the dentate gyrus is distinctly labeled whereas the molecular and polymorphic layers display weak or no immunostaining. The granule cells stained moderately to strongly for γ3 and the immunoreactivity was mainly in the soma of these cells, but could also be seen in the early branches of the granule cell dendritic trees, which extend into the lightly stained molecular layer (Figure 5H).
At the macroscopic level, medium to strong γ4 immunostaining was seen throughout the pyramidal layers of Ammon's horn and in the alveus (Figure 6A). The alveus stained strongly for γ4 as did the cell bodies in the pyramidal layer of all CA regions. This was observed more clearly at high power with a similar high level of staining of pyramidal cell bodies throughout the CA1-4 regions, but with the most intense perisomatic staining observed in the CA2/3 region (Figure, 6C, 6D, and 6E). Weak to moderate staining was observed throughout the neuropil of the strata surrounding the pyramidal layers in all regions. In the dentate gyrus, the granule cell layer appears to stain slightly more strongly than the adjacent molecular or polymorphic regions (Figure 6F). This also may be an artifact of the cell density in the granule layer rather than increased expression of the γ4 protein in the dentate granule cell bodies compared to their processes.
The effects of γ2 and γ4 on the biophysical properties of CaV2.1 calcium channels
Since the role of the stargazin-like proteins in relation to VDCC function remains controversial we examined the effects of γ2 and γ4 on the biophysical properties of CaV2.1 calcium channels. Together with the CaV2.1 α subunit, we co-expressed a β4 subunit with or without an α2δ2 subunit. These VDCC subunits are known to be highly expressed in cerebellar Purkinje cells [31–38], a cell type that, according to the immunohistochemistry data presented herein, strongly expresses both γ2 and γ4. Figure 7A and 7B show the current-voltage relations of CaV2.1/β4 and CaV2.1/β4/α2δ2 VDCCs respectively, expressed in Xenopus oocytes either in the presence or absence of γ2 or γ4. Neither γ2 nor γ4 produced a statistically significant shift in the half-maximal voltage of activation (V50act) of CaV2.1/β4 (Table 1). The slope factor (kact), maximum conductance (Gmax), and peak current amplitude at +10 mV were also very similar to controls on co-expression of either γ2 or γ4 in the absence the α2δ2 subunit. Co-expression of the α2δ2 subunit was sufficient to significantly increase the Gmax and peak current amplitude of a CaV2.1/β4 Ca2+ channel complex (Figure 7A and 7B). However, the increase was not affected by the additional co-expression of either γ2 or γ4.
No statistically significant differences were observed in the half-maximal voltage of steady-state inactivation (V50inact) or slope factor (kinact) of CaV2.1/β4 or CaV2.1/β4/α2δ2 VDCCs co-expressing γ2 or γ4 (Figure 7C, 7D and 7E and Table 2).
Differential distribution of human stargazin-like γ mRNAs
The northern blot analysis described herein detected the human γ2, γ3 and γ4 mRNAs only in the brain. Similar mRNA distributions and transcript sizes have been reported by in situ hybridization and northern blot for the mouse orthologues [1, 14, 19] and these data are supported by western blots . We do not however rule out the possibility that human γ2, γ3 and γ4 may be expressed in some non-CNS tissues that are not included on the multiple tissue northern blot used in this study. Other laboratories that employed highly sensitive reverse-transcriptase polymerase chain reaction expression analyses have reported that γ4 in particular is expressed in some non-neuronal tissues [13, 17]. The γ2 and γ4 transcripts were detected in most of the brain regions probed and mainly in the same tissues. However, in some regions where both isoforms are detected, for example the cerebellum or thalamus, γ2 expression is higher than γ4, whereas in the basal ganglia regions of putamen and caudate nucleus γ4 mRNA is the more highly represented transcript. γ3 is much more selectively detected, but its expression is coincident with both γ2 and γ4 in all regions in which it was detected by northern blot.
An additional observation was that these stargazin-like γ proteins are differentially detected in some of the nuclei that comprise the basal ganglia, a region believed to be involved in the planning and programming of movement, or more broadly in the processes by which intention is converted into voluntary action. The putamen has a heterogeneous neuronal γ population with γ4 mRNA possibly the most prevalent of the three γ transcripts investigated herein, as has been previously observed in both mouse and rat brain [14, 25]. The caudate nucleus also expresses transcripts for all three stargazin-like γ proteins, however the signal detected with the γ2 probe was extremely weak in comparison to those for γ3 and γ4. On the other hand, γ2 was the most prevalent species detected in substantia nigra, with faint detection of γ4 and no γ3 signal. No signals for any of the γ transcripts were detected in the sub-thalamic nucleus. Further immunohistochemical and electrophysiological investigation will be required to elucidate why γ2, γ3 and γ4 have such differential distributions in these nuclei.
In vitro expression of human stargazin-like proteins and antibody specificity
The transient expression of cloned human γ2, γ3 and γ4 cDNAs in COS-7 cells served several purposes: It determined that the human γ2, γ3 and γ4 cDNA clones expressed in vitro; it demonstrated the specificity of three anti-γ Abs, one generated to detect each of the three isoforms; it revealed that the stargazin-like γ2, γ3 and γ4 all localize to the plasma membrane of COS-7 cells when expressed either alone or in combination with other VDCC subunits and finally it showed that COS-7 cells do not endogenously express γ2, γ3 or γ4. Furthermore, because the anti-γ Abs, all of which were designed to detect epitopes in the C-terminus after the fourth predicted transmembrane segment, failed to stain COS-7 cells that had not been permeablized, it was established that the C-terminus is localized to the membrane and inside the cell. If predictions of secondary structure as envisaged by other laboratories are correct, a tetra-spanning transmembrane conformation will also place the N-terminus on the cytoplasmic side of the membrane [12, 16].
The distribution of the γ2, γ3 and γ4in cerebellum
This study is the first immunohistochemical analysis of stargazin-like γ proteins in the human CNS. In the cerebellum, we observed that γ2 was detected in molecular, granule and Purkinje cell layers as has been previously reported for mouse γ2 protein  and mRNA [1, 14, 19]. A major difference between human and rodent immunostaining patterns was observed for γ4, which was detected as very high levels in human cerebellar Purkinje cell bodies and processes, an observation not reported for rat or mouse [22, 25]. The detection of γ3 protein expression in the human cerebellum, albeit at extremely low levels, was similar to the γ3 mRNA detection patterns reported in mouse or rat cerebellum [19, 25] but disagree with the findings on our northern blots and the in situ hybridization data of Klugbauer et al. . The positive, albeit weak detection of γ3 protein in cerebellar interneurons and low levels detected in Purkinje cell bodies suggests that γ3 is expressed in distinct types of neurons in human cerebellum at levels too low to be detected by some hybridization conditions.
The almost inverse staining patterns of γ2 and γ4 in the dentate cerebellar nucleus (DCN) was striking. Much of the perisomatic neuropil surrounding the DCN somata consists of afferents from the Purkinje cells and is stained particularly strongly for γ4 while the DCN cell bodies stained strongly for γ2 but were devoid of γ4 staining. It is therefore a reasonable assumption that γ4 is pre-synaptically localized in the Purkinje cell afferents to the DCN whilst γ2 localizes to the post-synaptic regions of the DCN cell bodies. Indeed this observation holds with the finding that γ4 expressed well throughout Purkinje cell processes. γ4 also appears to be localized in the GABAergic neurons of the cerebellum more than the excitatory glutamatergic cell types. In addition to showing strong immunostaining throughout Purkinje cells, γ4 is detected in the interneurons of the molecular layer and also the Golgi interneurons of the granule layer. Whilst γ2 is also detected in all these cell types, the γ4 immunostaining is noticeably lower, if not absent from the excitatory granule cells, and is absent from the DCN cell bodies.
The distribution of γ2, γ3 and γ4in hippocampus
This study has determined that γ2, γ3 and γ4 show differential but overlapping expression patterns in the human hippocampus and dentate gyrus. As is the case for the cerebellum, the expression patterns of γ2 and γ4 more closely resemble one another than that of γ3. The γ2, and γ4 proteins were detected throughout the hippocampus and dentate gyrus, although there were variations in the staining of cell bodies, dendrites and neuropil in the different sub-fields. γ3 localized more specifically in the neuronal cell bodies of the hippocampus and dentate gyrus. This possibly indicates that γ2 and γ4 are involved in synaptic modulation of neurotransmission throughout the cell, whereas γ3 is solely involved in functions such as regulation of VDCCs or AMPA receptors in the cell soma.
CaV2.1/β4 VDCC currents are not modulated by human γ2 or γ4 co-expression in the presence or absence of an α2δ2subunit
The co-expression of γ2 or γ4 with CaV2.1/β4 VDCCs in the absence or presence of the α2δ2 subunit, did not significantly affect peak current amplitude or any of the activation or inactivation properties. These data agree closely with the findings of Chen et al.  who recorded whole cell Ca2+ currents from stg and wild type cerebellar granule cells. They reported that absence of γ2 neither altered the I-V relationship of the native whole cell Ca2+ current nor did it significantly modulate the steady-state inactivation properties of VDCC current compared to wild type. Although they did not use pharmacological agents to isolate specific components of the whole cell Ca2+ current which might have highlighted subtle changes particular to the P/Q-, N-, R-, or L-type currents present in this cell type [39, 40], it is unlikely that another known γ isoform could functionally substitute for γ2 to maintain normal VDCC function in that instance because distribution studies have shown that they are probably not expressed in this cell type [14, 19, 25]. This indicated that even if γ2 was associated directly or indirectly with a VDCC complex in cerebellar granule cells [20, 22], it did not modulate the high voltage activated VDCC I-V relationship or inactivation properties.
More recent patch clamp recordings from stg thalamic relay neurons showed a 45% increase in HVA VDCC peak current densities compared to wild type  consistent with a previous report that γ2 inhibited high voltage activated VDCC peak current amplitude by 37–40% when expressed in Xenopus oocytes . Why our data and those of Chen et al.  are so different from these results may be explained by differences in subunit combinations expressed in granule cells and thalamic relay neurons and between the two in vitro studies. Stargazin-like proteins might be able to directly modulate VDCC complexes consisting of particular subunit combinations, but are unable to reproduce this influence on other subunit complexes if they required additional interacting proteins not present in the Xenopus oocyte or cerebellar granule cells. The electrophysiological data therefore cannot be used as a strong argument to warrant considering these γ proteins as an integral part of high voltage-activated Ca2+ channels because modulation of current properties has not been reproducible between different studies. Nevertheless, there is biochemical evidence for the association of γ2 and γ3 with the N-type CaV2.2 channel [20, 22] and it is quite possible that whilst γ2, γ3 and γ4 readily associate with certain neuronal VDCC complexes, specific environmental conditions must be met for them to exert a measurable biophysical influence.
Human γ2, γ3 and γ4 stargazin-like proteins (or TARPs) are detected solely in the CNS. On the whole, their differential distributions closely parallel those of their rodent orthologues as observed by northern blot, in situ hybridization, western blot and immunohistochemistry [1, 14, 19, 22, 25] with some notable exceptions. The differential expression pattern of each isoform among the cell types present in human cerebellum and hippocampus predicts specific roles for each subtype in neuronal function, and possibly even segregated VDCC-γ or AMPA receptor-γ complexes . The results of our electrophysiology experiments support the notion that γ2, γ3 and γ4 stargazin-like proteins are not "subunits" of VDCCs in the true sense of the word. Nevertheless, we do not discount the possibility that they may interact with VDCCs and possibly influence trafficking, assembly or integration of VDCCs and AMPA-receptor function in their native environment or that to modulate VDCC current they require other factors not endogenously expressed in Xenopus oocytes.
cDNA sources and synthesis
Human brain total RNA was purchased from Invitrogen (Paisley, UK) and used to generate cDNA using the Superscript Pre-amplification System (Invitrogen) primed with random hexamers according to the manufacturer's instructions.
Isolation and cloning of the γ2, γ3 and γ4cDNAs
The complete open reading frame (ORF) of human CACNG2 cDNA was amplified from 25 ng human brain cDNA by PCR (cycling parameters: 98°C for 1 min, then 30 cycles of 98°C for 30 s, 55°C for 30 s, 72°C for 2 min, followed by a final 10 min extension step at 72°C) containing Pfu polymerase and 25 pmol each of the gene specific primers (GSPs), 5'-GCGGCCGCACCATGGGGCTGTTTGATC-3' and 5'-GCTAGCCTCGAGTTAGTGTTTATATAATGAAGAA-3'. These amplify the ORF of CACNG2, with a 5' extension of a Not I restriction site and partial Kozak sequence for initiation of translation in vertebrates (ACC)  and a 3' extension of Xho I and Nhe I restriction enzyme sites. Amplified fragments of the correct size were purified from agarose gels using the Qiaex II kit (Qiagen, Crawley, UK), and cloned into pCR2.1-TOPO vector (Invitrogen). Positive colonies identified by blue/white screening were confirmed by EcoR I restriction digest of purified plasmid and were sequenced on both strands using T7 (5'-TAATACGACTCACTAT AGGG-3') and M13R (5'-CAGGAAACAGCTATGAC-3') universal primers and gene specific primers in an automated dye terminator sequencer (Applied Biosystems, Warrington, UK).
The same procedures used to clone human CACNG2 were followed to clone CACNG3 and CACNG4, but using the primer pairs, 5'-CGGCCGCCACCATGAGGATGTGTGACAGAGGTA-3' and 5'-GCTAGCCTCGAGTCAGTTCAGACGGGCGTGG TG-3' to amplify CACNG3 ORF or, 5'-GCGGCCGCACCATGGTGCGATGCGACCGCG-3' and 5'-GCTAGCCTCGAGTCACACAGGGGTCGTCCGTC-3' to amplify CACNG4 ORF. These primer pairs contained the same restriction enzyme sites and if appropriate, the partial Kozak sequence  in their 5' extensions as were included in the CACNG2 primers. The human γ2, γ3, and γ4 cDNAs were subcloned into the pMT2 vector for expression in Xenopus oocytes and COS-7 cells .
Other cDNA clones
The following cDNAs were used: rabbit CaV2.1 (X57689), rat β4 (LO2315) and mouse α2δ2 (AF247139, common brain splice variant). All cDNAs were subcloned into expression vector pMT2 .
Multiple sequence alignments and basic local alignment search tool (BLAST) searches compared the protein sequences of all known stargazin-like γ proteins with one another and with other proteins in the public databases. This identified regions of lowest homology between γ2, γ3 and γ4 that were not present in any other known proteins. The following peptides, TARATDYLQASAITRIPS (γ2, amino acids 211–228), FHNSTPKEFKESLHNNPAN (γ3, amino acids 291–309) and VHDFFQQDLKEGFHVSMLN (γ4, amino acids 303–321), were synthesized by standard solid-phase techniques at Severn Biotech (Kidderminster, UK) to generate specific polyclonal antibodies (Abs). Each was coupled to the carrier protein tuberculin purified protein derivative (PPD) using sulpho- Succinimidyl4- [N-maleimidomethyl] cyclohexane-1-carboxylate (SMCC) (Pierce, Tattenhall, UK) via a Cys residue added at the N-terminus during synthesis. To raise polyclonal anti-γ2, anti-γ3 and anti-γ4 Abs, the resulting conjugates were used to immunize Bacille Calmette-Guerin (BCG)-sensitized Dutch rabbits at monthly intervals . The immune response was monitored by indirect enzyme-linked immunoabsorbent assay (ELISA) with free peptide-coated micro-titer plates. Immunoglobulins from the terminal bleeds were purified using immobilized peptide antigen columns (Sulfo-link, Pierce). Each anti-γ Ab was checked for specificity for its target by immunocytochemistry. COS-7 cells transfected with a single stargazin-like γ protein cDNA were examined for positive staining following incubation with the appropriate affinity purified Ab. Control slides were also examined for cross-reaction of the primary Ab with non-transfected COS-7 cells, stargazin-like γ proteins other than the target against which the primary Ab was intended to bind (data not shown) and the VDCC α1, α2δ, and β subunits used in this investigation. Finally, positive staining of target protein was abolished following overnight pre-incubation (at 4°C) of the primary Ab with a 10 × molar excess of the peptide against which it was raised (data not shown).
Cell culture and transfections
COS-7 cell were cultured as previously described . Transfection was performed using the Geneporter transfection reagent (Gene Therapy Systems, San Diego, CA). Cells were plated onto coverslips 2–3 h prior to transfection. The DNA and Geneporter reagent (2 μg and 10 μl, respectively) were each diluted in 500 μl of serum-free medium, mixed, and applied to the cells. The α1, β, α2δ and γ cDNAs were mixed and transfected in a 1:1:1:1 ratio by DNA weight. If a particular cDNA was absent from the transfection, substitution with blank pMT2 vector maintained correct subunit ratios. After 3.5 h, 1 ml of medium containing 20% serum was added to the cells, which were then incubated at 37°C for 3 days. Prior to staining, cells were re-plated using a non-enzymatic cell dissociation solution (Sigma, Dorset, UK) and maintained at 27°C for between 2 and 6 h.
COS-7 cells were fixed and permeablized for immunocytochemistry essentially as previously described . Primary Abs, affinity purified anti-γ2, anti-γ3 and anti-γ4 were used at 0.2 μg/ml. Secondary biotin conjugated or goat anti-rabbit (Sigma, Dorset, UK) Ab was applied at 5 μg/ml. Texas red-conjugated streptavidin was applied at 3.3 μg/ml. The nuclear dye 4', 6-diamidino-2-phenylindole (DAPI, 300 nM, Molecular Probes) was also used to visualize the nucleus. Cells were examined on a confocal scanning laser microscope (Leica TCS SP, Milton Keynes, UK). All images were scanned sequentially to eliminate cross-talk and photomultiplier settings kept constant in each experiment.
Human 12-lane multiple tissue blots and brain II and III blots (BD Biosciences Clontech) were hybridized at 65° C in ExpressHyb solution (BD Biosciences Clontech) according to the manufacturer's instructions. The [α32P] radiolabeled γ probes corresponding to nucleotides 597–814, 560–792 or 594–804 of the γ2, γ3 or γ4 ORFs respectively, were assembled according to the Strip-EZ DNA Probe Synthesis Removal Kit (Ambion (Europe) Ltd, Huntingdon, UK). The final stringency wash performed was 0.1 × Saline Sodium Citrate, 0.1% Sodium dodecylsulphate (SDS) at 65°C.
Human Brain Immunohistochemistry
Human brain tissue was obtained from two males aged 76 and 80 years whose causes of death were listed as metastatic carcinoma and left ventricular failure respectively. No prior history of brain disease was noted. The tissue, provided by the Cambridge Brain Bank Laboratory at the University of Cambridge, U.K., was collected using ethical consent procedures according to U.K. law. Sections were fixed in neutral buffered formalin and processed to paraffin wax. Paraffin-embedded wax blocks were sectioned on a Microm HM3555S microtome at 7 μm and underwent a microwave antigen retrieval procedure using a citrate buffer at pH 6.0 prior to immunostaining [45, 46]. Endogenous peroxidase was blocked with 3% hydrogen peroxide in water followed by non-specific protein blocking in 5% milk powder in phosphate buffered saline (PBS). Sections were incubated overnight with primary Ab (anti-γ2, 0.85 μg/ml; anti-γ3, 1.3 μg/ml; anti-γ4, 0.85 μg/ml) diluted with 3% goat serum and 0.1% Triton X-100 at 4°C in a moist chamber. Treatment of all sections post application of primary Ab was identical. Goat anti-rabbit (1:20 dilution, Biogenex, Wokingham, UK) link Ab was applied to all sections and incubated at room temperature for 20 min, followed by horseradish peroxidase-conjugated streptavidin (Biogenex, prediluted (1/20)) that was incubated for a further 20 min. Sections were visualized using 3,3'-diaminobenzidine (DAB) solution made up according to Vector kit SK-4100 (Peterborough, UK), and incubated with the sections for 2–10 min. In a few cases, sections were counterstained briefly in Mayer's hematoxylin prior to microscopic examination.
Negative control preparations performed in all immunohistochemistry experiments included replacement of primary antibodies with PBS only and preincubation of primary antibodies with the 100 μM corresponding immunizing peptide overnight at 4°C prior to immunostaining.
Microscopy and image analysis
Slides were analyzed using a Zeiss Axioplan Optical Microscope (Carl Zeiss Ltd., Welwyn Garden City, UK). All sections were viewed under bright-field illumination using × 2.5, × 10, × 20, or × 40 objectives and a stabilized light source. For each tissue section, both The Central Nervous System , and The Human Brain: an introduction to its functional anatomy  were used for reference, together with corresponding counterstained sections to locate and define nuclear groups. A JVC KY-F55B television camera attached to the microscope and a PC running AcQuis image capture software (Syncroscopy, Cambridge, UK) was used to obtain images of each section.
Adult female Xenopus laevis were killed by anesthetic overdose in a 0.25% solution of tricaine, decapitated, and pithed. Oocytes were removed and defolliculated by treatment with 2 mg/ml collagenase type IA in Ca2+-free ND96 saline containing (in mM): NaCl, 96; KCl, 2; MgCl2, 1; and HEPES, 5, pH-adjusted to 7.4 with NaOH for 2 hr at 21°C. Plasmid cDNAs for the different VDCC subunits were mixed in a weight ratio of 1:1:1:1, and ~10 nl was injected into the nuclei of stage V or VI oocytes. In control oocytes without a γ cDNA or in the absence of an α2δ cDNA, an equal volume of water was substituted in the mix. Injected oocytes were incubated at 18°C for 3–5 days in ND96 saline (as above plus 1.8 mM CaCl2) supplemented with 100 μg/ml penicillin, 100 IU/ml streptomycin (Invitrogen), and 2.5 mM Na pyruvate. Whole-cell recordings from oocytes were made in the two-electrode voltage-clamp configuration under continuous gravity-fed superfusion (~1 ml/min) with a chloride-free solution containing (in mM): Ba(OH)2, 10; TEA-OH, 80; NaOH, 25; CsOH, 2; and HEPES, 5 (pH7.4 with methanesulfonic acid). In all experiments, oocytes were injected with 30–40 nl of a 100 mM solution of K3-1, 2-bis (aminophenoxy) ethane-N, N, N', N'-tetra-acetic acid (BAPTA) in order to suppress endogenous Ca2+-activated Cl- currents. Recording microelectrodes, were pulled from thick-walled borosilicate glass capillary tubing with the following dimensions: 1.5 mm outer diameter, 1.0 mm bore diameter and with an internal 0.1 mm fiber (Plowden and Thompson, Stourbridge, UK). The TEVC pipettes were pulled using a P-87 Flaming/Brown microelectrode puller (Sutter Instrument Company, Novato, CA). Electrodes contained 3M KCl and had resistances of 0.3–2 MΩ. The holding potential (VH) was -100 mV. Membrane currents were recorded, amplified, low-pass filtered at 1 kHz using a Geneclamp 500 B amplifier, digitized through a Digidata 1200 interface (Axon Instruments, Foster City, CA) and stored on a PC using data acquisition software pClamp 6.02 (Axon Instruments). In all cases currents were leak subtracted on-line by a P/4 protocol. Additional analyses including calculation of means, standard error of the mean (S. E. M.), significance (unpaired Student's t-tests, where applicable) and curve fitting were calculated using Origin 5.0 (OriginLab Corporation, Northampton, MA). One-way analysis of variance (ANOVA) and post-hoc tests were performed using software available at http://faculty.vassar.edu/lowry/VassarStats.htm. Where mean values are presented they are shown as mean ± S. E. M. (with n depicting the number of oocytes from which the mean was calculated). Statistical significance was defined as P < 0. 05.
Current-voltage (I-V) relation curves generated from currents activated by a 200 ms long depolarizing pulse were fitted with a combined Boltzmann and linear fit function:
I = Gmax (V - Vrev) / (1 + exp(- (V - V50act) / k))
where I is the whole cell current amplitude, Gmax is the maximum slope conductance, V0.5act is voltage of the mid-point of activation, Vrev is the reversal potential and k is the slope factor for activation.
Steady-state inactivation data were generated from currents activated by a 100 ms long depolarizing pulse from the holding potential (VH) to 0 mV immediately after a 25 s conditioning pre-pulse between -100 and 0 mV. Current amplitudes were normalized to the maximum amplitude and fitted with a Boltzmann function of the form:
I / Imax = 1 / (1 + exp((V - V50inact) / k))
where I / Imax is the normalized peak current, V50inact is the voltage for the mid-point of inactivation, V is the conditioning voltage and k is the slope factor for inactivation.
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The Medical Research Council as part of an industrial collaborative PhD studentship with GlaxoSmithKline supported F. J. M. The Wellcome Trust provided additional support. The authors would like to thank Dr M. Rees (University College London) for the α2δ2 cDNA and acknowledge the assistance of Dr. Carles Cantì (University College London), and Dr. Chris Plumpton, Michael Hurle and Margaret Flint (GlaxoSmithKline, Stevenage, UK).
FJM carried out the cloning of the human γ2, γ3 and γ4 proteins, performed all the necessary practical and analytical procedures to acquire and process the primary data presented in this paper and drafted the manuscript. ACD and JJC participated in the conception, design and coordination of the study. All authors read and approved the final manuscript.
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