Transient expression of γ
, and γ
in COS-7 cells. A. Positive staining for γ2, γ3, or γ4 (red) is strongly localized to the membranes of permeablized cells transfected with the individual γ2, γ3, or γ4 cDNAs. Little immunostaining is observed in the cytoplasm between the membrane and nucleus (blue). B. Non-transfected cells did not stain for γ2, γ3, or γ4 using the Abs generated in this study. C. Cells transfected with γ2, γ3, or γ4 cDNA, but not permeablized during the staining process do not show immunoreactivity for the appropriate anti-γ Abs. D. When co-transfected together with the CaV2.1 and β4 VDCC subunit cDNAs, the expression patterns of γ2, γ3, or γ4 were unaltered. Scale bars in all panels represent 10 μm.