Figure 2

Transient expression of γ ₂ , γ ₃ , and γ ₄ in COS-7 cells. A. Positive staining for γ₂, γ₃, or γ₄ (red) is strongly localized to the membranes of permeablized cells transfected with the individual γ₂, γ₃, or γ₄ cDNAs. Little immunostaining is observed in the cytoplasm between the membrane and nucleus (blue). B. Non-transfected cells did not stain for γ₂, γ₃, or γ₄ using the Abs generated in this study. C. Cells transfected with γ₂, γ₃, or γ₄ cDNA, but not permeablized during the staining process do not show immunoreactivity for the appropriate anti-γ Abs. D. When co-transfected together with the Ca_V2.1 and β₄ VDCC subunit cDNAs, the expression patterns of γ₂, γ₃, or γ₄ were unaltered. Scale bars in all panels represent 10 μm.