Figure 3
Expression of the γ 2 , γ 3 , and γ 4 stargazin-like proteins in human cerebellum. A. γ2 immunoreactivity in the molecular layer (m) and part of the adjacent granule cell layer (g) in a cerebellar folium. Strong γ2 immunostaining was seen in the Purkinje cell somata (p) that continued into the dendrites, observed as moderate staining of the molecular layer neuropil. Cell bodies in the molecular layer with positive γ2 immunoreactivity are small interneurons (i). B. In the granule cell layer, the granule cells (g) are moderately stained for γ2 although the strongest immunostaining in this region is actually in the interneurons (i). C. The dentate cerebellar nucleus (dcn), displayed strong γ2 immunostaining in the cell bodies of this nucleus with only weak to moderate staining in the surrounding neuropil. In this particular section, cell nuclei are stained blue by Mayer's hematoxylin counterstain. D. Cerebellar folia displayed little to no γ3 immunostaining in the central white matter (wm) and molecular layer (m). Weak immunostaining was observed in the Purkinje cell bodies and the interneurons of the granule cell layer (g). E. Pre-absorption control, with the peptide immunogen for the γ3 Ab in the adjacent section to Fig. 3D. F. The cell bodies of the Purkinje cells (p) stained strongly for γ4 and this extended well into the Purkinje cell dendrites (d). The surrounding molecular layer neuropil (m) displayed light-moderate staining, as did the granule cell layer (g). Cell bodies in the molecular layer immunoreactive to the γ4 Ab were small interneurons (i). G. Granule cell layer interneurons (i) stained much more strongly for γ4 than the granule cells (g). H. The dcn gave a strong γ4 immunostaining signal in the perisomatic neuropil with only weak staining in the nucleus cell bodies. I. Immunizing peptide pre-absorption control for the γ2 Ab. Pre-absorption of the γ3 or γ4 Abs with their immunizing peptides produced almost identical results. Scale bars in all panels represent 25 μm.