- Methodology article
- Open Access
Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines
© Trapani and Korn; licensee BioMed Central Ltd. 2003
Received: 08 April 2003
Accepted: 02 July 2003
Published: 02 July 2003
Many molecular studies of ion channel function rely on the ability to obtain high quality voltage clamp recordings using the patch clamp technique. For a variety of channel types studied in mammalian cell heterologous expression systems, the lack of experimenter control over expression levels severely hinders the ability to obtain a high percentage of cells with an expression level suitable for high quality recordings. Moreover, it has been nearly impossible to obtain expression levels in mammalian cells well suited for single channel recordings. We describe here the use of an inducible promoter system in a stably transfected mammalian cell line that produces nearly 100% success in obtaining ion channel expression levels suitable for either whole cell or single ion channel studies.
We used a tetracycline-regulated expression system to control K+ channel expression in a CHO (Chinese hamster ovary) cell line. Current magnitudes within a reasonably narrow range could be easily and reliably obtained for either macroscopic or single channel recordings. Macroscopic currents of 1 – 2 nA could be obtained in nearly 100% of cells tested. The desired expression level could be obtained within just 2 to 3 hours, and remained stable at room temperature. Very low expression levels of transfected channels could also be obtained, which resulted in a >70% success rate in the ability to record single channel currents from a patch. Moreover, at these low expression levels, it appeared that endogenous channels produced little or no contamination.
This approach to controlling ion channel expression is relatively simple, greatly enhances the speed and efficiency with which high quality macroscopic current data can be collected, and makes it possible to easily and reliably record single channel currents in a mammalian cell heterologous expression system. Whereas we demonstrate the ability of this system to control expression levels of voltage-gated K+ channels, it should be applicable to all other channel types that express well in mammalian expression systems.
Many molecular studies of ion channel function rely on expression of cloned channels in mammalian cells. These studies usually utilize patch clamp techniques to examine the influence of experimental manipulations on the magnitude, gating kinetics and/or voltage-dependence of ionic currents passing through the channels. Under physiological conditions, cell membrane potentials vary between ~-90 mV and +60 mV. Consequently, studies of ion channel properties depend on the technical ability to accurately record current magnitudes between these membrane potentials. In general, high quality voltage clamp experiments in the whole cell configuration require currents in the range of 0.1 to 2.5 nA.
A significant problem with the use of mammalian cells is that expression levels are difficult to control. In transient transfection systems, channel expression levels are marginally controlled by the amount of cDNA used for transfection. In stable transfection systems, plasmids required for heterologous expression are incorporated into the host cell's genome. With stable lines, therefore, expression level is not under experimenter control. Due to this lack of control, expression levels are highly variable and often too high. For example, in HEK cells (human embryonic kidney cells, one typically used cell line) transiently transfected with potassium channel cDNA, ionic currents in cells from a single transfected population can range from 0 to >20 nA within the membrane potential range of interest. In our experience, when adequate amounts of cDNA are used to promote a reasonable transfection efficiency, 4 out of every 5 cells examined have currents larger than 2.5 nA at a membrane potential of interest. Thus, the practical result of this lack of control is that only 20% of examined cells can be relied upon to give accurate voltage clamp data. In stable cell lines, potassium channel expression levels often result in currents in the 5 to 10 nA range at 0 mV. This precludes quantitatively accurate voltage clamp recordings. Moreover, the complete lack of control over expression in stable lines makes it impossible to obtain the range of expression levels required for different types of experiments. This lack of control, in both transient and stable transfection systems, significantly hinders experimental progress.
A second problem that results from the lack of control over expression in mammalian cells is that it is extraordinarily difficult to perform studies of single ion channel currents. Single channel studies reveal important mechanistic information about channel gating that is impossible to obtain from examination of macroscopic currents. However, even at moderate expression levels, channel density is too high to obtain just one or two channels with a patch electrode of reasonable resistance.
What is needed to solve both of these problems is a technique that allows the experimenter to reliably generate cells that express channels at a density appropriate for a particular experiment. Because experimental needs can vary daily, an ideal solution would include the ability to control expression levels on a daily basis.
We report here the use of a simple method to reliably control ion channel expression on the day of recording. With the use of an inducible promoter system in a stable cell line, 100% of cells express channels over a very narrow, experimenter-chosen range of densities. Most importantly, expression can be held at 1) levels that produce current magnitudes appropriate for macroscopic current recordings or 2) a level that results in a channel density of less than one per square micron of cell membrane, which greatly facilitates the recording of single channel currents.
Results and Discussion
The chosen method had to accomplish four goals. First, channel expression levels had to be controlled within a reasonably narrow, desired range. Second, different ranges of expression had to be easily obtained. For example, a very low expression level is needed for recording single channel currents, a mid-range of expression is needed for macroscopic currents, and high expression levels are needed for gating current measurements. Third, any desired expression level had to be achieved on a day's notice. Finally, to facilitate scientific progress, it would be ideal if ~100% of cells expressed currents within the desired range.
The basic approach
To accomplish all of these goals, we produced stable mammalian cell lines with channel expression under the control of an inducible promoter [1–4]. Any cell line that is good for heterologous expression would be suitable for creating a stable cell line. In addition, the cell line of choice must be amenable to the inducible promoter system being used (c.f. ). We used CHO (Chinese hamster ovary) cells due to the extremely low expression level of endogenous ion channels . We purchased T-REx-CHO cells from Invitrogen Corp. (Carlsbad, CA). These cells are stably transformed with the pcDNA6/TR vector, which contains the tetracycline (Tet) repressor gene. We further transformed these cells with a pcDNA4/TO vector, which contained 1) a CMV promoter with two copies of the Tet operator 2 sequence, and 2) the cDNA encoding the channel gene of choice ligated into the multiple cloning site. Channel expression levels were then under the control of a competition between constitutively expressed Tet repressor protein and exogenously added tetracycline. In the absence of Tet, the Tet repressor protein is bound to the Tet operator and expression is prevented. Incubation of cells with Tet results in the displacement of the Tet repressor protein from the Tet operator, and expression occurs.
Production of current densities suitable for macroscopic current recordings
Thirty minutes appeared to be the borderline duration of Tet incubation required for initiation of expression (Fig. 2B). After a 30 minute incubation with Tet, 3/7 cells expressed no current at all above background. In contrast, after exposure to Tet for 60 minutes or more, 100% of cells expressed a current level above background.
Production of current densities suitable for single channel recordings
Figures 1 and 2A,2B,2C demonstrate that current magnitude can be kept within an ideal range for whole cell patch clamp recordings. For recording single ion channel currents, we needed to reliably obtain much lower channel densities. Cells maintained in normal FBS without added Tet displayed small but measurable currents (Fig. 2D). When cells were maintained in Tet-free media (see Methods) for up to 4 weeks, the observed current magnitude was reduced to <112 pA per cell at 0 mV. Normalized for cell size, these currents reflected a current density of less than 10 pA/pF, or <1 channel per μm2 (Fig. 2D). This density is well suited to recording currents through single channels with a standard, low resistance patch pipet, which has a tip diameter of 0.8 – 1 μm. (It should be noted that this minimal level of expression is likely to vary with different cell clones.)
Single channel recordings
The data in Fig. 3 demonstrated that few, if any, endogenous voltage-gated K+ channels were present in the stable CHO cell lines. Three observations strongly indicated that single channel currents obtained from stably transfected CHO cells also represented exclusively the transfected channels. First, previous whole cell recordings demonstrated that, when normalized for channel number, current magnitude in wild type Kv2.1 was half that of the mutant Kv2.1 channel . The single channel data obtained from the stably transfected CHO cells were consistent with these results. At 0 mV, in the presence of 0 mM external K+, single channel current magnitude in CHO cells containing wild type Kv2.1 averaged 0.35 ± 0.02 pA (n = 3). In cells containing Kv2.1 K356G K382V, single channel current magnitude averaged 0.72 ± 0.01 pA (n = 3). Second, under a variety of different experimental conditions, populations of cells transfected with either of the two channels displayed single channel conductances that varied by 5% or less (data not shown). Third, in patches with multiple channels, all single channel events displayed identical conductances. Thus, whereas care must always be taken, it appears that this system allows for the reliable recording of single channel currents from transfected cells with a low probability of contamination from endogenous channels.
For functional studies of cloned ion channels, two expression systems are commonly used. In one, Xenopus oocytes are transfected by injection with mRNA. In general, channel expression levels in oocytes can be controlled appropriately for both macroscopic and single channel recordings. Many investigators, however, choose to use mammalian cells as the heterologous expression system of choice. While there are a variety of advantages to mammalian cells for this purpose, standard transfection procedures, which involve the introduction of cDNA into the cell, provide only marginal, and often unsatisfactory, control of expression levels.
With the Tet-on inducible expression system in CHO cells, we were able to control macroscopic current magnitude within a very narrow window, over a large range of expression levels. With the appropriate exposure to Tet, currents could be obtained within an ideal range for whole cell patch clamp recording in essentially 100% of cells. When maintained in Tet-free media, cells expressed a low level of current, which was derived, perhaps entirely, from the stably transfected channel. This low level of expression was well-suited for recording currents through single channels in the cell-attached mode with low resistance patch pipets. The ability to control current magnitude at different levels solves two serious problems for patch clamp studies of ion channels expressed in mammalian heterologous expression systems. First, in whole cell studies, it dramatically increases productivity by increasing the percentage of cells that can be well clamped. Second, the ability to hold current density very low allows for the recording of single channel currents from mammalian cells, a nearly impossible task when uncontrolled expression techniques are used. Moreover, a relatively high percentage of recordings under Tet-free conditions yielded cells with 1 or 2 channels in a patch, which makes the ability to record single channel currents from the channel of choice, expressed in mammalian cells, routine.
Selection and maintenance of the stable cell line and control of expression
Stable cell lines were made for two voltage-gated K+ channels, wild type Kv2.1 and a mutant channel derived from Kv2.1 (Kv2.1 K356G K382V). For each channel type, 10 of ~40 cell foci that remained following transfection and exposure to antibiotics were selected and expanded. Four of the ten cell cultures for each channel were then tested for Tet-inducible channel expression. Seven of these eight cultures displayed Tet-induction, and displayed similar macroscopic current magnitudes in both the absence and presence of tetracycline.
Stable cell lines were kept under selective pressure by blasticidin (pcDNA6 contained the blasticidin resistance gene) and Zeocin (pcDNA4 contained the Zeocin resistance gene). Cells were maintained in a CO2 incubator (37°C, 8.5% CO2) in Ham's F12 media containing 10% fetal bovine serum (FBS; Hyclone Laboratories, Logan, UT), 1% penicillin-streptomycin, 10 μg/ml blasticidin and 250 μg/ml Zeocin. In some experiments, cells were maintained in Tet-free FBS (Hyclone Laboratories). Tet-free FBS contained < 300 pg/ml of Tet, determined by ELISA assays (Hyclone Laboratories). Normal FBS was unscreened for Tet. To initiate expression, Tet was added to the maintenance media at a concentration of 1 μg/ml. Expression levels were controlled by the duration of Tet incubation, as described in the Results section.
Channel expression was determined using standard whole cell patch clamp recordings, as described previously . Briefly, cells were removed from the incubator, and incubation media was replaced with a bath solution containing, in mM: 145 N-methylglucamine (NMG), 2 CaCl2, 1 MgCl2, 10 HEPES, 10 glucose, pH adjusted with HCl to 7.3, osmolality 330. The intracellular solution contained, in mM: 100 KCl, 35 NMG, 10 EGTA, 4 MgCl2, 1 CaCl2, 10 HEPES, pH adjusted with HCl to 7.3, osmolality 290. Once seal formation was achieved, cells were superfused by constantly flowing bath solution. Cells were clamped at a holding potential of -80 mV and currents activated by depolarization to 0 mV. In Fig. 4B and 4C, currents were recorded in the cell-attached patch configuration. In these experiments, the pipet contained intracellular solution, except that 100 mM NMG-Cl was substituted for KCl. The bath solution had 100 mM KCl substituted for NMG-Cl. This held the whole cell resting potential near 0 mV (data not shown), which enabled us to know the membrane potential across the patch. In cell attached patches, the pipet potential with respect to ground was held at +80 mV, and channels were activated by stepping the pipet potential to 0 mV. All currents were filtered at 2 KHz (Axopatch 200B amplifier, Axon Instruments, Union City, CA) and digitized at 10 KHz for whole cell recordings and 20 KHz for single channel recordings (pClamp 9, Axon Instruments). Recording pipets were fabricated from 8250 glass (Garner Glass, Claremont, CA), coated with Sylgard and firepolished. Pipet resistance was between 1.5 and 2.0 MΩ.
This work was supported by NIH NS41090.
- Yao F, Svensjo T, Winkler T, Lu M, Eriksson C, Eriksson E: Tetracycline repressor, tetR, rather than the tetR-mammalian cell transcription factor fusion derivatives, regulates inducible gene expression in mammalian cells. Hum Gene Ther. 1998, 9: 1939-50.PubMedView ArticleGoogle Scholar
- Perraud AL, Fleig A, Dunn CA, Bagley LA, Launay P, Schmitz C, Stokes AJ, Zhu Q, Bessman MJ, Penner R, et al: ADP-ribose gating of the calcium-permeable LTRPC2 channel revealed by Nudix motif homology. Nature. 2001, 411: 595-9. 10.1038/35079100.PubMedView ArticleGoogle Scholar
- McHugh D, Flemming R, Xu SZ, Perraud AL, Beech DJ: Critical intracellular Ca2+ dependence of transient receptor potential melastatin 2 (TRPM2) cation channel activation. J Biol Chem. 2003, 278: 11002-6. 10.1074/jbc.M210810200.PubMedView ArticleGoogle Scholar
- Howe JR, Skryabin BV, Belcher SM, Zerillo CA, Schmauss C: The responsiveness of a tetracycline-sensitive expression system differs in different cell lines. J Biol Chem. 1995, 270: 14168-74. 10.1074/jbc.270.23.14168.PubMedView ArticleGoogle Scholar
- Yu SP, Kerchner GA: Endogenous voltage-gated potassium channels in human embryonic kidney (HEK293) cells. J Neurosci Res. 1998, 52: 612-7. 10.1002/(SICI)1097-4547(19980601)52:5<612::AID-JNR13>3.3.CO;2-N.PubMedView ArticleGoogle Scholar
- Immke D, Korn SJ: Ion-ion interactions at the selectivity filter. Evidence from K+- dependent modulation of tetraethylammonium efficacy in Kv2.1 potassium channels. J Gen Physiol. 2000, 115: 509-18. 10.1085/jgp.115.4.509.PubMedPubMed CentralView ArticleGoogle Scholar
- Wood MJ, Korn SJ: Two mechanisms of K+-dependent potentiation in Kv2.1 potassium channels. Biophys J. 2000, 79: 2535-46.PubMedPubMed CentralView ArticleGoogle Scholar
- Consiglio JF, Andalib P, Korn SJ: Influence of pore residues on permeation properties in the Kv2.1 potassium channel. Evidence for a selective functional interaction of k+ with the outer vestibule. J Gen Physiol. 2003, 121: 111-24. 10.1085/jgp.20028756.PubMedPubMed CentralView ArticleGoogle Scholar
- Trapani JG, Korn SJ: Effect of external pH on activation of the Kv1.5 potassium channel. Biophys J. 2003, 84: 195-204.PubMedPubMed CentralView ArticleGoogle Scholar
- Andalib P, Wood MJ, Korn SJ: Control of outer vestibule dynamics and current magnitude in the Kv2.1 potassium channel. J Gen Physiol. 2002, 120: 739-55. 10.1085/jgp.20028639.PubMedPubMed CentralView ArticleGoogle Scholar
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