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Figure 4 | BMC Neuroscience

Figure 4

From: Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines

Figure 4

Recording single channel currents from cell-attached patches. (A) Whole cell recording from Kv2.1 K356G K382V after 120 min incubation with Tet. Current was recorded with an external solution containing 100 mM K+ and a pipet solution containing 0 K+. (B) Just prior to rupturing the patch for the recording in panel A, current was recorded across the membrane patch in cell-attached mode. (C) Cell-attached patch recording of a single ion channel from a cell maintained in Tet-free media for 44 days. Channel opening is in the upward direction.

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BMC Neuroscience

ISSN: 1471-2202

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