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Figure 1 | BMC Neuroscience

Figure 1

From: Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines

Figure 1

Time-dependent increase in channel expression with Tet incubation. Currents were recorded from CHO cells stably expressing the Kv2.1 channel. (A) Peak whole-cell current magnitude, evoked by depolarization to 0 mV, is plotted as a function of the duration of Tet incubation, which began at time 0. (B) Same as in A, except that the current magnitude was normalized to cell size, as determined by whole cell membrane capacitance. The insets in each panel illustrate the mean current values (± S.E.M., n = 3 – 12) at selected times during the first 180 min.

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