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Figure 2 | BMC Neuroscience

Figure 2

From: Control of ion channel expression for patch clamp recordings using an inducible expression system in mammalian cell lines

Figure 2

Long-term effects of Tet incubation on expression. (A) Cells were exposed to Tet in the incubator for 250 min, then removed from the incubator and kept in recording solution at room temperature. Recordings were made from a series of different cells over a period of 80 min to determine current density. The horizontal line is drawn at the mean density over the complete set of data. (B) Current density was determined at t = 250 min following the Tet incubation times shown on the abscissa. Values in parentheses denote the number of cells tested. (C) Current density was determined at t = 460 min following the Tet incubation times shown on the abscissa. (D) Cells were maintained in media containing no added Tet, plus either normal fetal bovine serum (FBS) or Tet-free serum for the number of days indicated on the abscissa.

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