Metabolic compartmentalization in the human cortex and hippocampus: evidence for a cell- and region-specific localization of lactate dehydrogenase 5 and pyruvate dehydrogenase
© Laughton et al; licensee BioMed Central Ltd. 2007
Received: 08 November 2006
Accepted: 23 May 2007
Published: 23 May 2007
For a long time now, glucose has been thought to be the main, if not the sole substrate for brain energy metabolism. Recent data nevertheless suggest that other molecules, such as monocarboxylates (lactate and pyruvate mainly) could be suitable substrates. Although monocarboxylates poorly cross the blood brain barrier (BBB), such substrates could replace glucose if produced locally.
The two key enzymatiques systems required for the production of these monocarboxylates are lactate dehydrogenase (LDH; EC126.96.36.199) that catalyses the interconversion of lactate and pyruvate and the pyruvate dehydrogenase complex that irreversibly funnels pyruvate towards the mitochondrial TCA and oxydative phosphorylation.
In this article, we show, with monoclonal antibodies applied to post-mortem human brain tissues, that the typically glycolytic isoenzyme of lactate dehydrogenase (LDH-5; also called LDHA or LDHM) is selectively present in astrocytes, and not in neurons, whereas pyruvate dehydrogenase (PDH) is mainly detected in neurons and barely in astrocytes. At the regional level, the distribution of the LDH-5 immunoreactive astrocytes is laminar and corresponds to regions of maximal 2-deoxyglucose uptake in the occipital cortex and hippocampus. In hippocampus, we observed that the distribution of the oxidative enzyme PDH was enriched in the neurons of the stratum pyramidale and stratum granulosum of CA1 through CA4, whereas the glycolytic enzyme LDH-5 was enriched in astrocytes of the stratum moleculare, the alveus and the white matter, revealing not only cellular, but also regional, selective distributions. The fact that LDH-5 immunoreactivity was high in astrocytes and occurred in regions where the highest uptake of 2-deoxyglucose was observed suggests that glucose uptake followed by lactate production may principally occur in these regions.
These observations reveal a metabolic segregation, not only at the cellular but also at the regional level, that support the notion of metabolic compartmentalization between astrocytes and neurons, whereby lactate produced by astrocytes could be oxidized by neurons.
In 1988, Fox and Raichle observed by positron emission tomography (PET) a mismatch between glucose uptake and oxygen consumption, raising the possibility that aerobic glycolysis, i.e. the nonoxidative consumption of glucose in the presence of oxygen, may occur in the brain during focal physiologic neural activity [1, 2]. Further support to this idea was brought by the observation that a lactate peak could be measured during physiological activation by 1H-magnetic resonance spectroscopy (MRS) [3, 4]. With the 2-deoxyglucose autoradiographic technique, glucose uptake has consistently been observed in the neuropil, i.e. in regions enriched in dendrites, axons and the astrocytic processes that ensheathe synapses, not the cell bodies [5, 6].
Since modern imaging techniques such as PET and functional magnetic resonance imaging (fMRI) are being increasingly used for clinical and fundamental biomedical research, it is of interest to understand cellular biochemical events underling observed signals.
These signals have been shown to result from the interactions between different cerebral cells, raising the concept of "neurovascular unit", including neurons, astrocytes and the vascular endothelium, whereby neuronal activity modulates vascular tension and metabolite delivery from the bloodstream . Apparently, the key cell for the control of vascular tension is the astrocyte  (for review, see ). For these authors, the vascular tonus is regulated via the stimulation of astrocytic glutamate receptors (mGluRs) triggering the release of vasoactive arachidonic acid metabolites. However, various teams [10–13] seem to think that the cytosolic NADH/NAD+ ratio plays a key role in the modulation of vascular tonus. This ratio is though to be in very close equilibrium with the pyruvate/lactate ratio  that depends on glycolysis.
Since pyruvate represents the end-point of glycolysis in mammalian cells, our goal in this study was to indirectly investigate its fate by localizing the two major enzymatic components of its energy production pathways, i.e. the pyruvate dehydrogenase complex (PDHC) and lactate dehydrogenase subunit M (LDH-5).
PDHC is a large, highly organized assembly of several different catalytic and regulatory subunits which catalyzes the oxidative decarboxylation of pyruvate to form acetyl-CoA, CO2 and NADH. Pyruvate dehydrogenase (PDH) catalyzes the irreversible entry of pyruvate into the tricarboxylic acid cycle and is therefore a marker for oxidative metabolism, whereas lactate dehydrogenase M subunit (LDH-5 subunit) is necessary for glycolysis to occur at high rate with production of lactate [15, 16]. Using immunohistochemistry, we seeked to examine their distribution in the human primary visual cortex and hippocampus. In these two regions, 2-deoxyglucose has been shown to accumulate in specific layers, i.e. the hippocampal stratum moleculare  and the layer IV of area 17 .
Specificity of the antibodies
Figure 1B illustrates the Western blot characterization of the mAb against PDH. On human hippocampal extracts, the antibody exclusively stained a band of approximately 43 kDa that is located in the mitochondrial fraction (fig 1B, 2) and failed to label the cytosolic fraction (fig 1B, 3). The same applied to the human occipital cortex (fig 1B, 4–6). The antibody stained a 43-kDa band from pyruvate dehydrogenase purified from porcine heart (fig 1B, 7) and did not stain purified LDH-5 extracted from rabbit muscle (fig 1B, 8, not shown as out of range of figure). Overall, the immunoreactivity at 43 kDa in human mitochondrial extracts strongly suggests that the monoclonal antibody recognizes the E1α subunit of human PDHC.
Human occipital cortex
In the human occipital cortex, area 17 was more specifically studied (figure 2). The PDH immunoreactivity was principally observed in layer IV, staining neuronal cell bodies (fig 2A, C). At the cellular level, virtually all neuronal bodies and dendrites were stained, whereas astrocytes were very faintly stained.
Using polyclonal antibodies (pAbs), we have previously shown that the LDH immunoreactivity of both M and H subunits is enriched in layers IV and VI of the occipital area 17 . Here, as shown in figure 2B and 2D, we confirm with a monoclonal antibody our previous observation of the apparently exclusive cellular distribution of LDH-5 immunoreactivity in astrocytes throughout the white matter and in cortical layer I of the visual cortex . In addition, protoplasmic astrocytes also appear in layer IV of area 17 (fig 2E) in close association with dendrites, providing a laminar staining similar to that observed by cytochrome oxidase histochemistry, immunohistochemistry and also by 2-deoxyglucose uptake studies [18, 19]. In the white matter of the occipital cortex, but also in that of parietal and frontal cortices virtually all astrocytes were intensively LDH-5 immunoreactive, suggesting that white matter astrocytes in general might be principally glycolytic.
Figure 3 shows the immunoreactivity of PDH (3A, 3C) and LDH-5 (3B, 3D) antibodies in the human hippocampus. It can clearly be observed a complementary staining of both antibodies, with intense immunostaining of LDH-5 in CA4, stratum moleculare and alveus while the stratum granulosum and the CA layers show no staining (fig 3B and 3C). PDH immunoreactivity is virtually present only in the stratum granulosum and the CA1-3 layers. Note the cellular, but also the regional metabolic compartmentalization of the two enzymes. The PDH immunolabeling was very similar to the labelling due to the pAb against LDH-1 that we have previously produced and characterized .
For both regions studied, a faint PDH immunoreactivity was revealed in astrocytes following longer incubation with the primary antibodies. In spite of signal amplification with TSA, no additional immunoreactivity was detected (data not shown).
In this article, we describe for the first time the immunohistochemical distribution of PDH and LDH-5 subunits in two regions of the human brain. Our monoclonal antibody to LDH-5 is highly specific that subunit of the enzyme and further confirms the results reported previously with the polyclonal antibody raised against rabbit muscle LDH-5 . However, very recently, O'Brien et al.  report that in isolated rat brain synaptosols and cytoplasms of primary cultured neurons and astrocytes that the LDH-5 isoform (i.e. the M subunit of LDH) is present also in neurons, findings that seem to be in contradiction with the data presented here. Of course however, cell cultures do not always reflect in vivo nerve cell phenotypes. In the human occipital cortex and hippocampus, the 2-DG autoradiographic technique has shown that glucose is principally taken up in specific layers, i.e. the layer IV of the occipital cortex  and the stratum moleculare of the hippocampal formation . In layer IV of the occipital cortex, a cellular compartmentalization was observed between LDH-5 and PDH immunolabeling, suggesting that astrocytes might principally produce lactate at a high rate and that the transformation of pyruvate to acetyl CoA might essentially occur in neurons. In the hippocampus, the same cellular segregation was observed, with the additional observation of a regional mismatch between both enzymes. Since 2-DG has clearly been observed to be taken up in the stratum moleculare , a layer enriched in LDH-5 immunoreactive astrocytes, this finding is consistent with the existence of a high glycolytic activity in astrocytes resulting in lactate production .
LDH-5 immunoreactivity was exclusively visualized in astrocytes in agreement with previous observation in human  and in astroglial cell cultures . No neurons revealed any LDH-5 immunoreactivity. The LDH-5 immunoreactive astrocytes were found in regions where 2-DG uptake is known to be maximal. However, LDH-5 immunoreactive astrocytes were also present in regions where no particular 2-DG uptake has been detected, i.e. the first cortical layer of occipital area 17, the hippocampal alveus and the white matter. It is worth noting that these areas are virtually devoid of neuronal profiles. Throughout the white matter, LDH-5 labelled astrocytes were numerous, and their distribution was homogenous. In the living rat, the uptake of 2-deoxyglucose has been found to be higher in grey matter than in white matter by a factor of approximately three-fold , whereas cytochrome oxidase activity is about eight- to twelve-fold higher in grey matter than in white matter . These observations suggest that the white matter might rely more on glycolytic metabolism than on oxidative phosphorylation and therefore produce lactate through LDH-5 subunit activity. Indeed, there is very little PDH immunoreactivity in the white matter, while the LDH-5 immunolabeling is abundant and restricted to astrocytes. Overall, the localization of the PDH immunoreactivity was similar to that previously observed [25, 26] and also overlapped previous reports of cytochrome oxidase immunolabeling, although patches were not observed .
Results reported in this article demonstrate the existence of a cellular and regional compartmentalization in two enzymes (LDH-5 and PDH) involved in glucose metabolism. At the cellular level, this compartmentalization is consistent with the notion of the existence of metabolic coupling between neurons and astrocytes, also known as the astrocyte neuron lactate shuttle hypothesis (ANLS).
Although the existence of the ANLS is still debated [27, 28], evidence has come from two-photon microscopy. Kasischke et al.  measured, at a cellular resolution, the variations of fluorescence due to the variation of the free NADH/NAD+ ratio. Their results shown that neuronal activity provokes oxidative metabolism in neurons closely followed by glycolytic metabolism in neighbouring astrocytes. Our present observations are fully consistent with this ex vivo data, whereby the intital oxidative metabolism is initiated by PDH catalysis and has a neuronal localization. The later the glycolytic activity is catalyzed by LDH-5 and is expressed in astrocytes. This late astrocytic glycolytic metabolism could be glutamate uptake stimulated . As mentioned previously variation in NADH/NAD+ in astrocytes may also be a key event modulating vascular tension . If this is the case, one step forward has been made in understanding the cellular events underlying signals emitted in modern imaging techniques.
In conclusion, describing not only the cellular but also the regional distribution of the typically glycolytic enzyme LDH-5 in comparison to the oxidative PDH distribution provides new insight to the highly metabolic coupling of the interactions between brain cells.
Preparation of tissues
All experimental procedures were approved by the ethics comity of the Geneva School of Medicine (Protocol n° 04-187/Psy 04-023). The brains of 10 control patients (4 females, 6 males, mean age 72 +/- 4, post-mortem delay < 12 h) with no history of neurological, metabolic or psychiatric disorder and without histopathological lesions were obtained at autopsy from the Hospitals of the University of Geneva School of Medicine. The brains were routinely screened for histopathological lesions, using classic histological stains and immunohistochemistry procedures as described in detail elsewhere . Some blocks of the hippocampal formation and occipital cortex were immediately frozen with CO2 for biochemical analyses, while others were fixed with 4% paraformaldehyde in 0.1 M PBS for 18 hours, then immersed in a solution containing 5% sucrose for 48 hours, and in 10% sucrose for another 48 hours before being stored at -80°C for less than a year.
Wistar male rats (IFFA CREDO, Les Oncins, France) were immunised by multiple injections of LDH-5 purified from rabbit muscle (SIGMA, Buchs, Switzerland. Hybridomas were produced by fusion of splenocytes with log-phase Sp2 myeloma cells in the presence of polyethylene glycol (MW 4000; Merck, Switzerland) according to the method reported by Lövenborg  and described in detail elsewhere . After screening by immunohistochemistry and western Blot (see below) for hybridomas producing monoclonal antibodies (mAbs) against LDH-5, positive hybridomas were propagated in a Biofarm 2000 system (Digitana, Switzerland).
The mouse mAbs respectively against the E1α and E2 subunits of human pyruvate dehydrogenase were purchased from Molecular Probes, Inc. Oregon, USA.
Western blot analysis
The mAb against LDH-5 was characterized by immunodot, immunohistochemistry on rabbit muscle and heart sections, and by western blot analysis as described previously . "Sodium-Dodecyl-Sulphate polyacrylamide gel electrophoresis" (SDS-PAGE) was performed according to the method of Laemmli . Fresh tissue extracts from human hippocampus (CA) and occipital cortex (CO) were homogenized on ice with diluted sample buffer. Purified proteins were all from Sigma, Switzerland and were also diluted in sample buffer. For the mAb against PDH characterization, mitochondrial fractions and cytosolic fractions of CA and CO were extracted by successive centrifugations in 0.01 M Tris-HCl with 0.32 M sucrose according to Hamberger et al. . Samples were heated at 100°C for 5 min, and approximately 50 μg of protein were loaded onto the gels. The extracts were then electrophoresed on polyacrylamide gels (12%) and transferred electrophoretically onto nitrocellulose membranes (Bio-Rad, Glattbrugg, Switzerland). After being blocked with non-fat dry milk and washed in phosphate buffer saline (PBS, pH 7.4), the membranes were incubated overnight with the antibodies. Detection of the bound antibodies was performed as described under "Immunohistochemistry" and/or using ECL+ with subsequent exposure to Hyperfilms (Amersham, Switzerland).
Molecular weight markers were used in all gels (Kaleidoscope and Low Range Prestained Standards from Bio-Rad).
For simple immunohistochemical labelling, adjacent 20 μm cryostat (Microm HM550) cut brain sections were mounted on 0.01% poly-L-lysine (PLL, Fluka, Switzerland) coated slides and incubated overnight at 4°C in PBS containing 3% bovine serum albumin (BSA, Sigma) and 0.3% Triton X with either the anti-LDH-5 supernatant diluted 1/500 or the anti-PDHE1α diluted 1/1000 (1/10000 for the anti-PDHE2) according to the manufacturers instructions. Following incubation, sections were rinsed in PBS and incubated for one hour at room temperature with horseradish peroxidase conjugated polyclonal rabbit anti-rat IgG (for LDH-5) or anti mouse IgG (for PDHE1α and PDHE2) (DAKO, Switzerland) diluted 1/100 in PBS containing 3% BSA and 0.3% Triton X. Following PBS washes, immunoreactions were revealed for 20 min at room temperature in PBS containing 0.02% 3,3'-diaminobenzidine (DAB, Sigma) and 0.002% H2O2.
For another series of simple and double labelling, 40 μm cryostat cut free floating sections were treated as above with the difference that the PDHE1α immunolabeling was revealed in 50 mM Tris-HCl containing 0.02% DAB and 0.002% H2O2 with 0.1% nickel ammonium sulphate . Double labelling was sequential, i.e. PDHE1α immunoreactivity was revealed before processing to LDH-5 immunolabelling.
An additional subset of sections were incubated for 48 hours in the two anti-PDH antibodies and processed as above or subjected to the Tyramide Signal Amplification (TSA) system (PerkinElmer life Sciences, Inc., Boston, USA) according to the manufacturer's instructions.
Our acknowledgements go to Mrs M Demiri-Surini and B. Greggio for their expertise in hybridoma cell culture.
- Fox PT, Raichle ME: Focal physiological uncoupling of cerebral blood flow and oxidative metabolism during somatosensory stimulation in human subjects. Proc Natl Acad Sci USA. 1986, 83 (4): 1140-1144. 10.1073/pnas.83.4.1140.PubMedPubMed CentralView ArticleGoogle Scholar
- Fox PT, Mintun MA, Reiman EM, Raichle ME: Enhanced detection of focal brain responses using intersubject averaging and change-distribution analysis of subtracted PET images. J Cereb Blood Flow Metab. 1988, 8 (5): 642-653.PubMedView ArticleGoogle Scholar
- Prichard J, Rothman D, Novotny E, Petroff O, Kuwabara T, Avison M, Howseman A, Hanstock C, Shulman R: Lactate rise detected by 1H NMR in human visual cortex during physiologic stimulation. Proc Natl Acad Sci USA. 1991, 88 (13): 5829-5831. 10.1073/pnas.88.13.5829.PubMedPubMed CentralView ArticleGoogle Scholar
- Sappey-Marinier D, Calabrese G, Fein G, Hugg JW, Biggins C, Weiner MW: Effect of photic stimulation on human visual cortex lactate and phosphates using 1H and 31P magnetic resonance spectroscopy. J Cereb Blood Flow Metab. 1992, 12 (4): 584-592.PubMedView ArticleGoogle Scholar
- Kadekaro M, Crane AM, Sokoloff L: Differential effects of electrical stimulation of sciatic nerve on metabolic activity in spinal cord and dorsal root ganglion in the rat. Proc Natl Acad Sci USA. 1985, 82 (17): 6010-6013. 10.1073/pnas.82.17.6010.PubMedPubMed CentralView ArticleGoogle Scholar
- Sokoloff L: Function-related changes in energy metabolism in the nervous system: localization and mechanisms. Keio J Med. 1993, 42 (3): 95-103.PubMedView ArticleGoogle Scholar
- Hawkins BT, Davis TP: The blood-brain barrier/neurovascular unit in health and disease. Pharmacol Rev. 2005, 57 (2): 173-185. 10.1124/pr.57.2.4.PubMedView ArticleGoogle Scholar
- Takano T, Tian GF, Peng W, Lou N, Libionka W, Han X, Nedergaard M: Astrocyte-mediated control of cerebral blood flow. Nat Neurosci. 2006, 9 (2): 260-267. 10.1038/nn1623.PubMedView ArticleGoogle Scholar
- Haydon PG, Carmignoto G: Astrocyte control of synaptic transmission and neurovascular coupling. Physiol Rev. 2006, 86 (3): 1009-1031. 10.1152/physrev.00049.2005.PubMedView ArticleGoogle Scholar
- Mintun MA, Vlassenko AG, Rundle MM, Raichle ME: Increased lactate/pyruvate ratio augments blood flow in physiologically activated human brain. Proc Natl Acad Sci USA. 2004, 101 (2): 659-664. 10.1073/pnas.0307457100.PubMedPubMed CentralView ArticleGoogle Scholar
- Vlassenko AG, Rundle MM, Raichle ME, Mintun MA: Regulation of blood flow in activated human brain by cytosolic NADH/NAD+ ratio. Proc Natl Acad Sci USA. 2006, 103 (6): 1964-1969. 10.1073/pnas.0510632103.PubMedPubMed CentralView ArticleGoogle Scholar
- Ido Y, Chang K, Woolsey TA, Williamson JR: NADH: sensor of blood flow need in brain, muscle, and other tissues. Faseb J. 2001, 15 (8): 1419-1421.PubMedGoogle Scholar
- Ido Y, Chang K, Williamson JR: NADH augments blood flow in physiologically activated retina and visual cortex. Proc Natl Acad Sci USA. 2004, 101 (2): 653-658. 10.1073/pnas.0307458100.PubMedPubMed CentralView ArticleGoogle Scholar
- Williamson DH, Lund P, Krebs HA: The redox state of free nicotinamide-adenine dinucleotide in the cytoplasm and mitochondria of rat liver. Biochem J. 1967, 103 (2): 514-527.PubMedPubMed CentralView ArticleGoogle Scholar
- Bittar PG, Charnay Y, Pellerin L, Bouras C, Magistretti PJ: Selective distribution of lactate dehydrogenase isoenzymes in neurons and astrocytes of human brain. J Cereb Blood Flow Metab. 1996, 16 (6): 1079-1089. 10.1097/00004647-199611000-00001.PubMedView ArticleGoogle Scholar
- Dawson DM, Goodfriend TL, Kaplan NO: Lactic Dehydrogenases: Functions of the Two Types. Science. 1964, 143 (3609): 929-933. 10.1126/science.143.3609.929.PubMedView ArticleGoogle Scholar
- Kennedy C, Des Rosiers MH, Sakurada O, Shinohara M, Reivich M, Jehle JW, Sokoloff L: Metabolic mapping of the primary visual system of the monkey by means of the autoradiographic [14C]deoxyglucose technique. Proc Natl Acad Sci USA. 1976, 73 (11): 4230-4234. 10.1073/pnas.73.11.4230.PubMedPubMed CentralView ArticleGoogle Scholar
- Wong-Riley MT, Hevner RF, Cutlan R, Earnest M, Egan R, Frost J, Nguyen T: Cytochrome oxidase in the human visual cortex: distribution in the developing and the adult brain. Vis Neurosci. 1993, 10 (1): 41-58.PubMedView ArticleGoogle Scholar
- Humphrey AL, Hendrickson AE: Background and stimulus-induced patterns of high metabolic activity in the visual cortex (area 17) of the squirrel and macaque monkey. J Neurosci. 1983, 3 (2): 345-358.PubMedGoogle Scholar
- O'Brien J, Kla KM, Hopkins IB, Malecki EA, McKenna MC: Kinetic Parameters and Lactate Dehydrogenase Isozyme Activities Support Possible Lactate Utilization by Neurons. Neurochem Res. 2007, 32 (4–5): 597-607. 10.1007/s11064-006-9132-9.PubMedView ArticleGoogle Scholar
- Pellerin L, Magistretti PJ: Food for thought: challenging the dogmas. J Cereb Blood Flow Metab. 2003, 23 (11): 1282-1286. 10.1097/01.WCB.0000096064.12129.3D.PubMedView ArticleGoogle Scholar
- Tholey G, Roth-Schechter BF, Mandel P: Activity and isoenzyme pattern of lactate dehydrogenase in neurons and astroblasts cultured from brains of chick embryos. J Neurochem. 1981, 36 (1): 77-81. 10.1111/j.1471-4159.1981.tb02379.x.PubMedView ArticleGoogle Scholar
- Sokoloff L: Relation between physiological function and energy metabolism in the central nervous system. J Neurochem. 1977, 29 (1): 13-26. 10.1111/j.1471-4159.1977.tb03919.x.PubMedView ArticleGoogle Scholar
- Hevner RF, Liu S, Wong-Riley MT: A metabolic map of cytochrome oxidase in the rat brain: histochemical, densitometric and biochemical studies. Neuroscience. 1995, 65 (2): 313-342. 10.1016/0306-4522(94)00514-6.PubMedView ArticleGoogle Scholar
- Milner TA, Aoki C, Sheu KF, Blass JP, Pickel VM: Light microscopic immunocytochemical localization of pyruvate dehydrogenase complex in rat brain: topographical distribution and relation to cholinergic and catecholaminergic nuclei. J Neurosci. 1987, 7 (10): 3171-3190.PubMedGoogle Scholar
- Bagley PR, Tucker SP, Nolan C, Lindsay JG, Davies A, Baldwin SA, Cremer JE, Cunningham VJ: Anatomical mapping of glucose transporter protein and pyruvate dehydrogenase in rat brain: an immunogold study. Brain Res. 1989, 499 (2): 214-224. 10.1016/0006-8993(89)90769-5.PubMedView ArticleGoogle Scholar
- Chih CP, Roberts EL: Energy substrates for neurons during neural activity: a critical review of the astrocyte-neuron lactate shuttle hypothesis. J Cereb Blood Flow Metab. 2003, 23 (11): 1263-1281. 10.1097/01.WCB.0000081369.51727.6F.PubMedView ArticleGoogle Scholar
- Fillenz M: The role of lactate in brain metabolism. Neurochem Int. 2005, 47 (6): 413-417. 10.1016/j.neuint.2005.05.011.PubMedView ArticleGoogle Scholar
- Kasischke KA, Vishwasrao HD, Fisher PJ, Zipfel WR, Webb WW: Neural Activity Triggers Neuronal Oxidative Metabolism Followed by Astrocytic Glycolysis. Science. 2004, 305 (5680): 99-103. 10.1126/science.1096485.PubMedView ArticleGoogle Scholar
- Pellerin L, Magistretti PJ: Glutamate uptake into astrocytes stimulates aerobic glycolysis: a mechanism coupling neuronal activity to glucose utilization. Proc Natl Acad Sci USA. 1994, 91 (22): 10625-10629. 10.1073/pnas.91.22.10625.PubMedPubMed CentralView ArticleGoogle Scholar
- Bouras C, Hof PR, Giannakopoulos P, Michel JP, Morrison JH: Regional distribution of neurofibrillary tangles and senile plaques in the cerebral cortex of elderly patients: a quantitative evaluation of a one-year autopsy population from a geriatric hospital. Cereb Cortex. 1994, 4 (2): 138-150. 10.1093/cercor/4.2.138.PubMedView ArticleGoogle Scholar
- Lövenborg U: Monoclonal Antibodies: Production and Maintenance. 1982, London: Heinemann W. Medical BooksGoogle Scholar
- Charnay Y, Golaz J, Vallet PG, Bouras C: Production and immunohistochemical application of monoclonal antibodies against delta sleep-inducing peptide. J Chem Neuroanat. 1992, 5 (6): 503-509. 10.1016/0891-0618(92)90005-B.PubMedView ArticleGoogle Scholar
- Laemmli UK: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature. 1970, 227 (259): 680-685. 10.1038/227680a0.PubMedView ArticleGoogle Scholar
- Hamberger A, Blomstrand C, Lehninger AL: Comparative studies on mitochondria isolated from neuron-enriched and glia-enriched fractions of rabbit and beef brain. J Cell Biol. 1970, 45 (2): 221-234. 10.1083/jcb.45.2.221.PubMedPubMed CentralView ArticleGoogle Scholar
- Leger L, Charnay Y, Burlet S, Gay N, Schaad N, Bouras C, Cespuglio R: Comparative distribution of nitric oxide synthase- and serotonin-containing neurons in the raphe nuclei of four mammalian species. Histochem Cell Biol. 1998, 110 (5): 517-525. 10.1007/s004180050313.PubMedView ArticleGoogle Scholar
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