Histone H4 deacetylation plays a critical role in early gene silencing during neuronal apoptosis

Nuclear HDAC activity is increased after ONC

Nuclear HDAC activity was measured in retinal nuclear protein extracts isolated at 1, 3, 5, and 7 days following ONC (Figure 1A). No significant change was detected in fellow control eyes (OD). Experimental eyes (OS) exhibited an ~50% increase in activity at day 5 post ONC (P = 0.0014). Activity was also significantly higher than control eyes at 7 days post ONC (P = 0.0006), although lower than day 5 experimental eyes. Nuclear HDAC activity in experimental and control eyes was completely inhibited by trichostatin A (TSA), indicating the presence of predominantly class I and II HDACs in this fraction (Figure 1A).

Dose-dependent inhibition of nuclear HDAC activity was also performed with different inhibitors to help evaluate which HDACs were active both before and after ONC. TSA-mediated inhibition of control and crush nuclear extracts from day 5 retinas showed a dose-dependent decrease in HDAC activity with an IC₅₀ ranging from 15 to 22 nM (Figure 1B). A similar loss of activity was observed in extracts treated with the selective class I inhibitor, valproic acid (data not shown). To further refine which class I HDACs may be contributing to the retinal nuclear activity, we repeated this experiment using apicidin, which is selective for HDACs 2 and 3. HDAC activity was also nearly completely inhibited with apicidin, yielding an IC₅₀ ranging from 1.88 to 2.55 nM (Figure 1C). These values agreed with the reported IC₅₀ value for HDAC3 (2.5 nM) using this inhibitor [26] and suggest that HDACs 2 and 3 contribute the majority of nuclear HDAC activity in the retina.

In addition to measuring nuclear HDAC activity, we also characterized which HDACs were expressed in the mouse retina by both mRNA and protein analysis. HDACs 1-5 were selectively examined because they have all been reported to be active in nuclei and are known to affect histone acetylation levels [27]. By RT-PCR and western blot analysis, HDACs 1, 2, 3, and 5, but not HDAC4 were detected in normal retinas (Figure 2A). At the protein level, bands of 59 kD and 49 kD, corresponding to HDACs 2 and 3, respectively, appeared to be most abundant. This is similar to a previous report of the pattern of HDACs expressed in normal brain tissue [27]. To examine the change in HDAC expression in response to ONC, quantitative RT-PCR (qPCR) analysis was conducted on samples isolated from several time points following surgery (Figure 2B). By 1 day post ONC, the mRNAs for Hdacs 2 and 3 doubled (P = 0.0098 and P = 0.0089, respectively), while transcripts for Hdacs 1 and 5 showed modest, but not significant increases. At 3 days post ONC, only Hdac3 transcripts remained significantly elevated (P = 0.0024). By 7 days post ONC, no significant elevation of any of the examined Hdacs was detected.

HDACs 2 and 3 in injured retinas

Since both Hdac 2 and 3 transcripts showed a significant increase following ONC and the majority of HDAC activity was sensitive to apicidin, we characterized these HDACs in the retina before and after ONC. To determine the localization of these isoforms, immunofluorescence was performed on retinal cryosections from control and crush eyes. Both HDAC2 and HDAC3 were present in cells of the ganglion cell layer (GCL) and inner nuclear layer (INL) of control eyes (Figure 3A). At higher magnification of the GCL of control retinas (Figure 3B, top panels), HDAC2 colocalized with 4,6-diamindino-2-phenylindole (DAPI), indicating that it was nuclear, consistent with reports that this is an exclusively nuclear protein [27]. In contrast, HDAC3 labeling was diffuse and appeared to be predominantly cytoplasmic with minimal overlap with DAPI labeling. This was also in agreement with previous reports that HDAC3 contains both a nuclear localization signal and a nuclear export signal [27], and can exist in both the cytoplasm and nuclei of cells [28].

Because subcellular localization of HDACs is a common mechanism of controlling HDAC activity [29], we also examined the distribution of HDAC2 and HDAC3 after ONC. In sections from retinas 3 days post ONC (Figure 3B, lower panels), HDAC2 remained localized to nuclei of the GCL. HDAC3 localization in the GCL, however, appeared to change after ONC, with cells showing both cytoplasmic and nuclear localization, as determined by colabeling with DAPI staining (Figure 3B and additional file 1: Localization of HDACs 2 and 3 before and after optic nerve crush). To confirm these findings, western blot analysis was conducted on nuclear and cytoplasmic fractions isolated from control and crush retinas. As shown in Figure 3C, a band at 59 kD, corresponding to HDAC2, was present in the nuclear fractions of both control and ONC retinas. A 49 kD band, corresponding to HDAC3, was present in the cytoplasmic fraction from control retinas, but was both cytoplasmic and nuclear in the experimental crush retinas consistent with the nuclear translocation of this protein after ONC. As a control for the fractionation, the blots were also probed for acetylated histone H4 and GAPDH as nuclear and cytoplasmic controls, respectively.

Histone H4 acetylation in the GCL decreases following ONC

Histones H3 and H4 are substrates of HDACs 2 and 3. In particular, deacetylation of at least 5 different lysine residues of H4 have been implicated in transcriptional silencing [24]. To determine if there was a decrease in acetylation of histones in injured RGCs, concomitant with the increase in HDAC activity and increased nuclear presence, we examined retinal cryosections taken from control and experimental retinas with a polyclonal antibody against pan-acetylated histone H4 (AcH4). Figure 4A shows low magnification images of the INL and GCL (left panels), and higher magnification images of just the GCL (right panels). Nuclear labeling was detected in both the INL and GCL of the control retinas (top panels). After ONC, the label intensity remained consistent in the INL, but there was an apparent and progressive decrease in labeling of the GCL. High magnification images (Figure 4A, right panels) indicated that the decrease in label intensity was not only due to a loss of cells in this layer, but also to a decrease in the labeling of individual cells within this layer. To verify the decrease in label intensity of individual nuclei in the GCL, fluorescent pixel intensity of cells in this layer were measured and normalized to pixel intensity of AcH4 labeling in the INL of the same section. Compared to control eyes, the average AcH4 label intensity of GCL nuclei in experimental retinas progressively decreased to ~45% by 5 days post ONC (Figure 4B, P < 0.0001 for days 1, 3, and 5 vs. control retinas). By 7 days post ONC, many RGCs are in the later stages of apoptosis [30]. Consistent with this, we observed an actual increase in the average AcH4 label intensity of cells remaining in this layer at 7 days. Although this average was still significantly below control levels (P = 0.04), it may reflect that unaffected amacrine cells made up a larger proportion of nuclei assayed at this time point.

Characterization of HDAC3 translocation and deacetylation of histone H4 in dying cells

The increase in nuclear HDAC3 localization and an apparent decrease in histone H4 acetylation in some cells of the GCL following ONC is consistent with the concept of widespread histone deacetylation taking place in dying RGCs, which are the principal cell type affected by the crush procedure. Importantly, we wanted to verify that these changes were characteristic of dying cells. To address this, we first identified dying cells in retinal cryosections with an antibody against phosphorylated H2AX (γH2AX). The phosphorylation of the histone H2A variant, H2AX, is a known marker of apoptosis, and its appearance coincides with early onset DNA damage that precedes mitochondrial involvement in the cell death program [31]. In the GCL of injured retinas, γH2AX underwent a progressive change in localization that allowed us to group the cells into three stages of γH2AX labeling (Figure 5A). Stage I labeling was principally found in control retinas and in some GCL cells in injured retinas and was characterized by little to no γH2AX labeling except for a densely labeled spot associated with the nucleoli. Stage II was distinguishable by strong perinuclear labeling, while stage III had strong nuclear labeling. Stage II labeled cells began to appear in the injured GCL as early as 1 day post ONC. Progressively after this, an increasing proportion of cells exhibited stage III labeling. By 5-7 days post ONC, 50-60% of the cells in this layer were either stage II or stage III for γH2AX, consistent with estimates that RGCs make up 60% of the cells in the GCL (Figure 5B) [32, 33]. To verify labeling in RGCs, we also retrogradely labeled them with fluorogold prior to ONC. After crush surgery, an estimated 85% of the fluorogold positive cells exhibited stage II or stage III γH2AX labeling (Figure 5C).

Figure 6 illustrates the change in HDAC3 and γH2AX labeling in the retina as RGCs progressed through apoptosis. HDAC3 appeared to be cytoplasmic in control cells (Figure 6A), and shortly after ONC, was found co-localized with stage II γH2AX labeled cells in the cytoplasm (Figure 6B). In other cells, however, HDAC3 staining was present in the nuclei, while γH2AX staining was still localized as a perinuclear ring in the cytoplasm (Figure 6C). In mid-to late stages post ONC, double-labeled cells in the GCL exhibited stage III γH2AX labeling and nuclear localization of HDAC3 (Figure 6D-F).

Because of the strictly nuclear presence of AcH4, the colocalization of AcH4 and γH2AX was much different than that observed for HDAC3. In control eyes, the nuclei were strongly labeled for AcH4 and γH2AX was only present as a spot that was associated with the nucleoli (Stage I, Figure 7A). Early after ONC, RGCs with stage II γH2AX localization also presented with strong nuclear labeling with AcH4 (Figure 7B, C). In stage III labeled cells, γH2AX and AcH4 colocalization was initially yellow in merged images, but progressed to orange, then red, indicative of both an increase in γH2AX intensity and a decrease in AcH4 labeling (Figure 7D-F). The nuclear localization of this co-labeling was confirmed with DAPI staining, which also indicated that cells with nuclear γH2AX and decreased AcH4 staining exhibited fragmented and condensed nuclei typical of apoptotic cells (See additional file 2: The deacetylation of histone H4 occurs in cells with nuclear γH2AX and DNA fragmentation).

Promoter deacetylation is associated with a downregulation of gene expression in injured RGCs

To correlate the observed decrease in histone H4 acetylation with gene silencing, we determined if H4 deacetylation occurred in transcriptionally significant sites, such as the promoter regions of genes that are downregulated in RGCs in response to ONC. Following ONC, a number of genes are known to decrease in expression. A retrospective analysis of the literature indicated that the rapid decrease in transcript abundance for a majority of genes roughly follows an exponential decay curve (Figure 8A). To verify this in a controlled setting, we developed a mini qPCR array of several mRNAs expressed in RGCs and used it to monitor the change in transcript abundance in retinas over the first 7 days post ONC. Similar to the literature reports, this prospective study also showed the exponential decay of RGC transcripts (Figure 8B). Two sets of genes were examined for chromatin immunoprecipitation (ChIP) analysis. The first group included Thy1, Brn3b, Nrn1, and Fem1c, which are expressed predominately by RGCs in the retina, as well as the anti-apoptotic gene BclX [3–5, 11, 14, 15]. This group of genes represented a subset of genes that exhibit a reported decrease in expression after ONC. The second group of genes, Bim and cJun, were examined because they undergo an increase in expression following ONC [5, 18]. ChIP assays, with antibodies against acetylated H4, were performed on retinas from control and crush eyes followed by qPCR to quantify the genomic DNA that was collected. The quantification of promoter DNA associated with acetylated histone H4 is shown in Figure 9 and is expressed as a ratio of experimental: control retinas. All ratios have been normalized to day 0 controls. Surprisingly, promoter regions for both down-regulated and up-regulated genes showed a significant decrease in histone acetylation 1 day post ONC, with the exception of the promoter for cJun (Figure 9A). Three days post ONC, however, the acetylation pattern of promoter histones was changed and only down-regulated genes exhibited a decrease in promoter acetylation (Figure 9B), while promoter H4 acetylation for cJun had remained at day 0 levels and levels for Bim had significantly increased ~2-fold. A similar pattern of promoter acetylation observed 3 days after ONC was also evident on day 5 post ONC (Figure 9C), except that Bim H4 promoter acetylation had increased further to ~3-fold the level detected in day 0 retinas.

Inhibition of HDAC activity blocks ONC-induced silencing of the Fem1c ^R3 reporter gene

Although promoter histone deacetylation is associated with silenced genes in RGCs, these experiments do not conclusively demonstrate that this epigenetic change is the controlling mechanism for transcriptional downregulation. To address this, we examined if inhibitors of HDAC activity could block the ONC-mediated downregulation of RGC-specific gene expression. For these experiments, we used Fem1c^Rosa3 (R3) mice, which contain the βGeo promoter trap reporter in the first intron of the Fem1c gene. Previously, we showed that mice express βGEO in an RGC-specific manner [12]. Additionally, we have observed a 75% decrease in βGEO total protein and enzyme activity [12], and a 50% decrease in Fem1c transcript levels, by 5 days post ONC (Figure 8A, B). Thus, the R3 reporter allows for the rapid detection and quantification of changes in RGC gene expression.

HDAC activity in the retina was inhibited by pretreatment of mice with TSA given as a single intraperitoneal (i.p.) injection, 24 hours before ONC. Western blot analysis of AcH4 levels in extracts of total retinal protein (Figure 10A) confirmed inhibition of HDAC activity, which was exemplified by hyperacetylation of H4. The effects of TSA were detected as quickly as 2 hours after injection and persisted as long as 7 days post injection. R3 gene expression was assessed by βGEO solution assays 5 days post ONC (Figure 10B). ONC resulted in a 55-75% decrease in βGEO enzyme activity by 5 days after surgery. TSA treated mice, however, exhibited significantly more activity at this time point than mice receiving no injection or DMSO injections (P = 0.016 and P = 0.003, respectively). In a separate series of experiments, TSA was also administered as a single intravitreal injection at the time of ONC surgery. Whole-mounted retinas from these experiments exhibited βGEO staining in RGCs consistent with the solution assays (Figure 10C). Taken together, these data suggested that TSA was able to attenuate the silencing of the Fem1c gene.

Inhibition of HDAC activity attenuates cell loss following ONC

Although histone deacetylation plays a role in modulating gene silencing during apoptosis, it is unknown if this process is a critical stage in the progression of the cell death program. To address this, we injected mice (i.p.) with the HDAC inhibitor, TSA, 24 hours prior to ONC. Retinas were then examined 2 weeks after surgery, which represents a point when there is normally significant cell loss [33]. The mice that underwent crush alone, or mice that received an injection of DMSO prior to ONC, exhibited comparable losses of 36.4 ± 3.4% and 31.2 ± 2.9% of cells in the GCL (P = 0.13, Figure 11A). Conversely, mice that received TSA prior to ONC showed a significant attenuation of cell loss in the GCL (14.4 ± 5.3%) as compared to both crush alone and crush with DMSO (P = 0.005 and P = 0.01, respectively). Representative Nissl-stained whole mounts of retinas from a control eye and each of the three treatments are shown in Figure 11B. Although cell loss was attenuated by treatment with TSA, surviving cells did exhibit signs of atrophy such as somal shrinkage.

Modulation of the HAT and HDAC activity balance during neurodegeneration

In healthy cells, HAT and HDAC activities are balanced to regulate transcriptional activity [24]. The disruption of this balance, as demonstrated through the use of HDAC inhibitors, can lead to apoptosis principally in rapidly dividing cells [42, 43]. Disruption of the HAT:HDAC balance also appears to play a role in neurodegenerative diseases, albeit by a mechanism that appears to be different from the lethal imbalances that cause cancer cell death. Rather than decreases, relative increases in HDAC activity contribute to the progression of neuronal apoptosis in several disease models [37, 44, 45]. One of the consequences of this imbalance could be an overall decrease in histone acetylation, which leads to a decrease in gene expression. Studies in which HDAC activity is suppressed by HDAC inhibitors, presumably restoring the HAT:HDAC balance, show that this treatment is able to attenuate neuronal apoptosis. Several groups investigating models of polyglutamine expansion neurodegenerative diseases, such as Huntington's disease, for example, have used HDAC inhibitors to prevent cell loss [38, 46–48]. In these models, the relative increase in HDAC activity has been hypothetically attributed to a decrease in HAT activity resulting from the sequestration and degradation of the acetyltransferase, CREB binding protein (CBP) [38, 46], while HDAC activity levels remain unchanged [49]. This model of neurodegeneration implies that the relative increase in HDAC activity is a passive consequence of the selective loss of CBP. Our data, although consistent with the idea that HDAC activity is relatively increased, suggest that this change is reflective of an active increase in nuclear HDAC levels in dying RGCs, associated with both a modest increase in HDAC gene expression and the translocation of an active protein. In fact, nuclear HAT activity assays show no significant changes in overall acetyltransferase activity in retinas harvested from eyes after ONC (data not shown).

The overall importance of HDAC activity in the process of RGC death can be probed with HDAC inhibitors such as TSA. TSA pretreatment was able to attenuate the down regulation of at least one gene normally expressed in RGCs (Fem1c ^R3 ). Consistent with the other reports showing a protective effect in models of neurodegeneration, TSA was also able to provide a modest protective effect to RGCs after ONC. The underlying cause for this latter effect is not known and may be due to a variety of factors, such as stabilizing the balance between HAT and HDAC activity or allowing for increased acetylation of factors such as Sp1, which has been shown to be neuroprotective in a model of hypoxic stress [50]. Conversely, it is equally possible that maintaining normal gene expression may have a secondary protective effect to the RGCs. For example, previously we showed that the anti-apoptotic gene BclX was down-regulated after optic nerve crush in rats [14]. Preventing this decrease could result in an increase in RGC resistance to a damaging stimulus by antagonizing the actions of proteins like BAX.

Although several studies have linked an increase in HDAC activity to neuronal death, others have demonstrated that the overexpression of HDACs can be neuroprotective. Chen and Cepko showed that the overexpression of HDAC4 led to increased protein stability of the transcriptional activator HIF1α, which had a protective effect in a model of photoreceptor degeneration [51]. A related study demonstrated that part of the beneficial effect of HDAC4 on HIF1α transcriptional activity was due to HIF1α's increased ability to bind the histone acetyltransferase, p300 [52], which could be beneficial during neurodegeneration when fewer active HATs are available [37, 44]. In these cases, the protective effect of increased HDAC activity appeared to be restricted to cytoplasmic activity on non-histone substrates.

Irrespective of the principal function of HDAC activity during cell death, the phenomenon of gene silencing is likely going to act as a barrier to regaining normal cell function in neuroprotective strategies. Bax-deficient RGCs, which are completely resistant to apoptosis after ONC, for example, can remain in a genetically silent, heterochromatic state for months following injury (unpublished data). Similarly, inhibition of HDAC activity using TSA, still resulted in some cell atrophy characterized by soma shrinkage. Shrinkage of RGCs after optic nerve damage has been described by others [53], including in Bax^-/- RGCs [54]. This atrophy response may be indicative of the apoptotic volume decrease described in some neuronal cell types [55], which is considered to be a very early event in the apoptotic program and is regulated by a rapid efflux of intracellular potassium in many neurons, including retinal ganglion cells [56, 57]. The effects we observe in TSA-treated mice suggest that epigenetic changes leading to gene silencing is downstream of the apoptotic volume decrease. Nevertheless, a complete understanding of the early changes in affected ganglion cells remains an important consideration for neuroprotective strategies. Clearly, regaining normal cell function will ultimately require reactivation of normal gene expression, meaning reversal of epigenetic changes associated with silenced genes.

Experimental animals and optic nerve crush

All mice were handled in accordance with the Association for Research in Vision and Ophthalmology statement for the use of animals for research. The majority of experiments were conducted on CB6F1 mice, which have been used in the past by our group to quantify cell loss and changes in RGC transcript levels, as a result of ONC. In some experiments, however, ROSA3, a substrain of C57BL/6 mice [12], were used to utilize the expression of βGEO marker protein as a way to more precisely quantify RGC-specific gene expression. These latter mice express βGEO as the result of transcription of the Fem1c gene, which is predominantly RGC-specific in the retina. They also exhibit similar kinetics of cell loss observed in the CB6F1 strain. For both strains, a random mixture of males and females between the ages of 4-6 months were used. ONC was performed unilaterally as described previously to initiate degeneration of the retinal ganglion cells [30]. Retinas were harvested 1, 3, 5, 7, or 14 days post ONC as indicated.

In some cases, retinal ganglion cells were retrogradely labeled with the tracer dye, fluorogold (Molecular Probes, Eugene, OR). Labeling was performed by first exposing the superior collicli on each side of the brain and placing a small piece of gel-foam, soaked in 0.9% NaCl containing 2% fluorogold, to each exposed surface. ONC surgery was performed 3 days after dye application.

HDAC activity assay and nuclear protein extraction

Nuclear proteins were extracted from whole retinas according to Andrews and Faller (1991). Protein concentration was determined using a BCA protein assay kit (Pierce, Rockford, IL). HDAC activity assays were performed using a Fluor de Lys kit (BIOMOL International, Plymouth Meeting, PA). Triplicate samples containing 4 μg of protein each were loaded in an opaque 96-well plate with Fluor de Lys substrate at a final concentration of 150 μM. Following a 20 minute incubation at room temperature, 1 × Fluor de Lys developer with trichostatin A was added to stop the reaction and develop the fluorescent signal. Plates were read using a CytoFluor plate reader at 360 nm excitation and 440 nm emission wavelengths (Perkin Elmer, Waltham, MA). All samples were corrected to a buffer-only blank and normalized to the HeLa extract controls.

Western blot analysis

Western blot analysis on nuclear and cytoplasmic retinal protein fractions (see above) was performed as previously described [58] with the following modifications. Each fraction was loaded as a single lane (500 μg) on separate12% polyacrylamide gels and transblotted onto Immobilon P (Millipore, Inc, Billerica, MA). Membranes were stained with Ponceau S, cut into strips, and probed with various antibodies. Rabbit polyclonal antibodies for HDACs 1-5 (Santa Cruz, city CA) and anti-acetyl-histone H4 ChIP grade rabbit antiserum (Millipore, Inc) were used at a 1:100 dilution. Goat anti-rabbit secondary antibodies, conjugated to alkaline phosphatase, were used at a 1:500 dilution (Jackson ImmunoResearch Laboratories Inc, West Grove, PA). Blots were color developed using NBT and BCIP and digitally scanned.

Evaluation of transcripts in the retina by quantitative PCR

Immunofluorescence and quantification of AcH4

Indirect immunofluorescence on 5 μm thick retinal cryosections was done as described previously [60]. Primary antibodies included γH2AX (monoclonal, Millipore, Inc), HDAC2, HDAC3, and AcH4 (previously mentioned) and were used at a 1:100 dilution. Secondary antibodies used were goat anti-rabbit with a Texas Red label (1:100) and goat anti-mouse with a FITC label (1:100) (both from Jackson ImmunoResearch Laboratories). The images were obtained using a Zeiss Axioplan 2 Imaging microscope with Axiovision 4.6.3.0 software (Carl Zeiss MicroImaging, Inc., Thornwood, NY) and viewed in Adobe Photoshop.

To quantify AcH4 staining, nuclear pixel intensity was measured in the ganglion cell (GCL) and inner nuclear layers (INL) using the outline function of the Zeiss Axiovision software. A minimum of 99 cells was counted in each layer and the mean pixel intensity per nucleus was calculated as a function of the nuclear area (in μm²). The calculated pixel intensity of GCL nuclei was normalized to the calculated pixel intensity of nuclei of the INL and the final calculated intensity was expressed as a ratio of crush:control.

Chromatin immunoprecipitation (ChIP) assays

Acetyl-histone H4 ChIP assays were performed as outlined by the manufacturer (Millipore). In each assay, 6 retinas were pooled and half of each sample was mixed with either AcH4 antibody or normal rabbit serum for a control (Jackson ImmunoResearch Laboratories). The supernatant obtained from the normal serum samples following immunoprecipitation was regarded as the input. DNA from immunopreciptates was unlinked from protein complexes and purified further by phenol/chloroform extraction. Samples were analyzed in triplicate using qPCR as described above. The data obtained from qPCR were analyzed by subtracting the normal serum samples from the AcH4 immunoprecipitated samples and converting this to a percentage of the total input. These numbers were then expressed as a ratio of crush:control and normalized to the day 0 values.

HDAC inhibitor studies

To inhibit HDAC activity in the retina, TSA was injected either intraperitoneally (1 mg/kg in DMSO) 24 hours prior to ONC surgery or intravitreally (1 μl of 20 μM TSA in DMSO) immediately after ONC surgery. Vehicle injections consisted of an equal volume of DMSO. The effects of TSA on gene expression were conducted on Fem1c ^R3/+ mice, which express the βGEO enzyme predominantly in RGCs in the retina. The level of βGeo expression was analyzed using two methods. Firstly, the level of βGEO activity in individual retinas was quantified by β-Galactosidase solution assay (Promega, Madison, WI) 5 days post ONC. The plates were read with an EL_X800 microplate reader (Bio-Tek Instruments Inc., Winooski, VT). Duplicate samples of each eye was measured and total activity was calculated after subtraction of the β-Galactosidase activity measured in wild-type mice and corrected to the amount of total protein loaded in each sample. Secondly, βGEO expressing cells were identified histochemically, 5 days post ONC, by staining retinal preparations with X-Gal, followed by whole mounting as previously described [12]. To assess the effects of TSA on RGC loss, a 1 mg/kg intraperitoneal injection of TSA was administered 24 hours prior to ONC of adult CB6F1 mice. Two weeks after ONC, cells in the GCL were Nissl-stained and counted and compared to controls [33].

Statistical analysis

Data was collected from a minimum of 3 independent samples in all experiments, and shown as the mean ± Standard Error in graphs. All statistical analyses were performed using the Student's t-test with statistical significance set at P ≤ 0.05.