Table 1 Primers for ChIP and qPCR analysis
From: Histone H4 deacetylation plays a critical role in early gene silencing during neuronal apoptosis
Gene Name | Primer Sequences | Size of product (bp) | Positiona |
|---|---|---|---|
Hdac1 | 5'-GTCAAAGGAGCCCACGCCAG-3' | 603 | +555/+1157 |
| Â | 5'-GTTGACAGCTTCGGGGAGGC-3' | Â | Â |
Hdac2 | 5'-ATGGCGTACAGTCAAGGAGG-3' | 359 | +209/+568 |
| Â | 5'-AGCAACTGAACCACCCGTGG-3' | Â | Â |
Hdac3 | 5'-GTATGACAGGACTGACGAGG-3' | 168 | +528/+695 |
| Â | 5'-TTTCCTTCCCACCACAGAGG-3' | Â | Â |
Hdac4 | 5'-CATCCCCAAGCCAAGCGAGC-3' | 619 | +1521/+2140 |
| Â | 5'-CCGTCTGTAGCTCCTCCAGG-3' | Â | Â |
Hdac5 | 5'-CTGTGCCTCATCAGGCCCTG-3' | 642 | +1919/+2560 |
| Â | 5'-CCACGCTCAGCTTCTGCTGC-3' | Â | Â |
Thy1 | 5'-CTGGAATCAAAGGTGTGAGC-3' | 190 | -288/-98 |
promoter | 5'-CCCCTCTCTTTATCCCCTTC-3' | Â | Â |
Brn3b | 5'-CTGAGGTCTGAAGCCAGAGC-3' | 266 | +17/+282 |
promoter | 5'-ACGCCTGCTTGCTGTTCAGG-3' | Â | Â |
Fem1c | 5'-TCCGCAGCCTTTCAGGATGC-3' | 188 | -255/-67 |
promoter | 5'-AGTGCGCCTGCGTACTAAGG-3' | Â | Â |
Nrn1 | 5'-AACAATCGAGTGGGCGCACC-3' | 251 | -137/+114 |
promoter | 5'-AAGTACAGCCTCACGCCCACG-3' | Â | Â |
BclX | 5'-ATCTGGTCGATGGAGGAACC-3' | 214 | +93/+326 |
promoter | 5'-AATCTATCTCCGGCGACAGC-3' | Â | Â |
Bim | 5'-AAAGCAAGGGCGGAGGGACG-3' | 238 | -369/-132 |
promoter | 5'-CTGTCCTGCAGTTTGCGTGC-3' | Â | Â |
cJun | 5'-AACACAAGCCGAAGCTGAGC-3' | 263 | -265/-2 |
promoter | 5'-AGTCCTTATCCAGCCTGAGC-3' | Â | Â |