Skip to main content

Table 1 Primers for ChIP and qPCR analysis

From: Histone H4 deacetylation plays a critical role in early gene silencing during neuronal apoptosis

Gene Name Primer Sequences Size of product (bp) Positiona
Hdac1 5'-GTCAAAGGAGCCCACGCCAG-3' 603 +555/+1157
  5'-GTTGACAGCTTCGGGGAGGC-3'   
Hdac2 5'-ATGGCGTACAGTCAAGGAGG-3' 359 +209/+568
  5'-AGCAACTGAACCACCCGTGG-3'   
Hdac3 5'-GTATGACAGGACTGACGAGG-3' 168 +528/+695
  5'-TTTCCTTCCCACCACAGAGG-3'   
Hdac4 5'-CATCCCCAAGCCAAGCGAGC-3' 619 +1521/+2140
  5'-CCGTCTGTAGCTCCTCCAGG-3'   
Hdac5 5'-CTGTGCCTCATCAGGCCCTG-3' 642 +1919/+2560
  5'-CCACGCTCAGCTTCTGCTGC-3'   
Thy1 5'-CTGGAATCAAAGGTGTGAGC-3' 190 -288/-98
promoter 5'-CCCCTCTCTTTATCCCCTTC-3'   
Brn3b 5'-CTGAGGTCTGAAGCCAGAGC-3' 266 +17/+282
promoter 5'-ACGCCTGCTTGCTGTTCAGG-3'   
Fem1c 5'-TCCGCAGCCTTTCAGGATGC-3' 188 -255/-67
promoter 5'-AGTGCGCCTGCGTACTAAGG-3'   
Nrn1 5'-AACAATCGAGTGGGCGCACC-3' 251 -137/+114
promoter 5'-AAGTACAGCCTCACGCCCACG-3'   
BclX 5'-ATCTGGTCGATGGAGGAACC-3' 214 +93/+326
promoter 5'-AATCTATCTCCGGCGACAGC-3'   
Bim 5'-AAAGCAAGGGCGGAGGGACG-3' 238 -369/-132
promoter 5'-CTGTCCTGCAGTTTGCGTGC-3'   
cJun 5'-AACACAAGCCGAAGCTGAGC-3' 263 -265/-2
promoter 5'-AGTCCTTATCCAGCCTGAGC-3'   
  1. a Indicates position (in base pairs) relative to the transcriptional start site (+1)