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Intravenous methylprednisolone or immunoglobulin for anti-glutamic acid decarboxylase 65 antibody autoimmune encephalitis: which is better?



Patients positive for anti-glutamic acid decarboxylase 65 (GAD65) antibodies have attracted increasing attention. Their clinical manifestations are highly heterogeneous and can be comorbid with tumors. Currently, there is no consensus on the therapeutic regimen for anti-GAD65-associated neurological diseases due to the clinical complexity, rarity and sporadic distribution. We reported six anti-GAD65 autoimmune encephalitis (AE) patients who received intravenous methylprednisolone (IVMP) or immunoglobulin (IVIG) or both. Then, we evaluated the therapeutic effect of both by summarizing results in previous anti-GAD65 AE patients from 70 published references.


Our six patients all achieved clinical improvements in the short term. Unfortunately, there was no significant difference between IVMP and IVIG in terms of therapeutic response according to the previous references, and the effectiveness of IVMP and IVIG was 45.56% and 36.71%, respectively. We further divided the patients into different subgroups according to their prominent clinical manifestations. The response rates of IVMP and IVIG were 42.65% and 32.69%, respectively, in epilepsy patients; 60.00% and 77.78%, respectively, in patients with stiff-person syndrome; and 28.57% and 55.56%, respectively, in cerebellar ataxia patients. Among 29 anti-GAD65 AE patients with tumors, the response rates of IVMP and IVIG were 29.41% and 42.11%, respectively. There was no significant difference in effectiveness between the two regimens among the different subgroups.


Except for stiff-person syndrome, we found that this kind of AE generally has a poor response to IVMP or IVIG. Larger prospective studies enrolling large numbers of patients are required to identify the optimal therapeutic strategy in the future.


Glutamic acid decarboxylase 65 (GAD65), an intracellular antigen that is highly expressed in pancreatic β-cells and the presynaptic terminal of inhibitory neurons, is becoming increasingly important both clinically and experimentally [1,2,3,4]. Patients with high serum and cerebrospinal fluid (CSF) titers of anti-GAD65 antibodies (Abs) have been proven to be clinically heterogeneous, including those with stiff-person syndrome (SPS), cerebellar ataxia (CA), limbic encephalitis (LE), nonlimbic autoimmune encephalitis (AE), autonomic neuropathy and other multifarious neurological disorders [5, 6]. Furthermore, tumors have been identified in several cases, indicating that anti-GAD65 Abs are associated with paraneoplastic neurological syndrome [7]. Even more perplexing, anti-GAD65 Abs are frequently comorbid with one or more systemic autoimmune diseases and other Abs [5, 8, 9]. Because of the clinical heterogeneity, together with highly variable complexity, low prevalence and sporadic distribution, performing large-scale clinical trials are challenging. Therefore, there is currently no global consensus on the therapeutic regimen, mainly including corticosteroids, immunoglobulin, plasma exchange and other immunosuppressant drugs; all of the available experience is based on case reports. However, management may vary widely among different medical centers, even when opposing recommendations are made [9,10,11].

In this study, we retrospectively analyzed the clinical characteristics of six anti-GAD65 AE patients in our tertiary epilepsy center who presented with different clinical injury sites and severity. To date, no study has indicated which treatment is better for anti-GAD65 AE patients when comparing intravenous methylprednisolone (IVMP) and intravenous immunoglobulin (IVIG). Thus, we analyzed the chosen treatments for all anti-GAD65 AE patients by searching PubMed, and we compared the efficacy of IVMP with that of IVIG. The purpose of the study is to raise the awareness of this disease and guide clinical therapies.

Patients and Methods

Six subjects from Beijing Tiantan Hospital gave written informed consent for participation and written consent to permit the publication of clinical details. The study was approved by the Medical Ethics Committee of Beijing Tiantan Hospital, Capital Medical University, and was carried out in accordance with the Declaration of Helsinki.

We conducted a search on PubMed for articles up to April 2019 and used the title/abstract search terms “encephalitis” and “GAD” or “glutamic acid decarboxylase”. A total of 133 references were retrieved. The criteria for enrollment were as follows: patients with anti-GAD65 Abs received a therapy of IVMP or IVIG or a combination of both, and we were able to obtain the response to the treatment. We were not particularly concerned about whether patients used antiepileptic drugs (AEDs) because the effect of AEDs was very limited [12, 13]. IVMP or IVIG meant that the patients received high-dose methylprednisolone or immunoglobulin pulse therapy, respectively, but the duration was variable, often by more than 5 days. Reference screening was conducted by two experienced neurological doctors, Tao-Ran Li and Rui-Juan Lv. Forty-nine articles, including 38 case (series) reports and 11 research articles, were included on the basis of their relevance to the inclusion criteria. In addition, a back-search of reference lists from retrieved publications was also conducted to identify other potentially relevant articles. As a result, 21 other articles, including 13 case (series) reports and 8 research articles, were also included.

We eliminated many references for the following reasons. First, we excluded studies for which it was difficult to assess the efficacy of methylprednisolone, immunoglobulin or a combination of methylprednisolone and immunoglobulin because patients received immunosuppressive therapy in addition to IVMP or IVIG meanwhile, such as plasma exchange [14,15,16], immunoadsorption [17], azathioprine [18], rituximab [19], mycophenolate mofetil [20] or others [21]. Second, we excluded studies for which we could not obtain the patients’ response to IVMP or IVIG or their combination because of the ambiguous descriptions or the inaccessible follow-up information in the original literature [22,23,24,25,26,27]. Third, we excluded studies for which the different clinical manifestations of patients responded inconsistently to treatment; for example, in patient D in the literature [28], seizure frequency did not respond to IVMP, while CA improved. Fourth, we excluded studies for which patients took prednisone or dexamethasone only orally [19, 29,30,31,32,33]. Fifth, we excluded those patients with concurrent Rasmussen encephalitis [34, 35].

The 70 references included in the statistics are listed in Additional file 1. We marked the references containing patients who presented with seizures, SPS or CA and references containing patients who had coexisting tumors or received combination therapy.

Statistical analysis

We counted the number of patients who responded to IVMP or IVIG or a combination of both in the literature. At the same time, we also counted the number of patients who did not respond to IVMP, IVIG or a combination of both. The clinical treatment for anti-GAD65 AE patients was diverse and complex, and most patients underwent a variety of treatment options. In many cases, patients changed into the second treatment plan since the first was ineffective or because of illness relapse; therefore, the same patient was likely to be assigned two statistical data points. For example, if one patient made no response to IVMP while responding to IVIG, we counted once that IVIG was effective and counted once that IVMP was ineffective. If one patient responded to combination therapy (not including that there was a time interval between IVMP and IVIG), we counted that combination therapy was effective once instead of calculating once separately that both IVMP and IVIG were effective. If one patient was unresponsive to combination therapy, we counted once separately that both IVMP and IVIG were ineffective. We completely followed the information obtained in the literature and objectively collected data according to the original authors’ standpoints.

Statistical analyses were performed with SPSS 22.0 software (SPSS, Chicago, IL, USA). Differences were evaluated by the Chi squared test or Fisher’s exact test. P < 0.05 was considered to be statistically significant.


Patients from our center

Six illustrative anti-GAD65 AE cases from our center are comparatively described in Table 1, and we include two typical examples for a detailed description below owing to spatial confinement. The six patients were all right-handed and were born at term to nonconsanguineous Chinese parents, with no abnormal antenatal or postnatal issues of note, and reached developmental milestones appropriately. Except for patients 2, 5 and 6, their past medical histories were unremarkable, and their family histories of seizures or neurological and immune disorders were also not special except that of patient 2. They all presented seizures as the initial symptom; however, their intracranial lesions and subsequent clinical manifestations were different as follows: patient 1 was characterized as having typical LE, patient 2 had obvious brain atrophy and features of multiple different clinical syndromes due to a long-term disease course, the remaining four patients mainly presented with medial temporal lobe injury in imaging (except patient 3), and all patients were characterized as having drug-resistant epilepsy (patient 4 also had SPS). The average time from onset to immunotherapy for the six patients was approximately 3 months, 18 years, 20 months, 19 years, 10 years and 5 years, respectively. They received different treatment regimens, all showing various degrees of improvement in the short term.

Table 1 Clinical characteristics of six GAD65 positive-AE patients from our center

Patient 1: typical LE

A 35-year-old female reported sudden onset of nocturnal generalized tonic–clonic seizures (GTCSs) 3 months ago; these GTCSs lasted approximately 1 min, with 5 episodes in total. In addition, she also had an inexplicable sense of fear, several times per day. She received adequate doses of levetiracetam, and the effect was not obvious. During the past 2 months, she experienced 3–4 conscious nauseous sensations per week. Initial neurological examination revealed only a decrease in calculation capacity. The Montreal Cognitive Assessment (MoCA) score was 22 (primary school degree) on a scale ranging from 0 to 30. Routine examinations of blood and CSF were normal. Comprehensive onconeural and neuronal surface Abs screening in the serum and CSF, detected by the cell-based transfection immunofluorescence assay method, showed only positive anti-GAD65 Abs. Brain magnetic resonance imaging (MRI) showed abnormal signals of the right medial temporal lobe (Fig. 1a). The long-term video electroencephalogram (EEG) showed significant frequent sharp waves, slow waves and sharp-slow complex waves in the right frontal and temporal regions during the interictal phase (Fig. 2). During the ictal phase, we found that the EEG rhythmic changes first appeared in the right central, parietal, posterior temporal and midline regions, accompanied by clinical seizures approximately 1 s later, manifested as a nauseous feeling and oropharyngeal automatism with impaired awareness, and the seizures lasted approximately 50 s (Fig. 3). The frequency of the above episodes was 2–3 times/day. Therefore, the patient was diagnosed with anti-GAD65 LE, and a 5-day course of IVMP, 500 mg/day, was initiated and gradually reduced. Subsequently, she took prednisone (1 mg/kg/day) and levetiracetam (750 mg/q12h) for maintenance therapy. The clinical improvement was remarkable. Four months later, she received a 5-day course of IVIG, 400 mg/kg/day, for consolidation therapy. Currently, she takes only levetiracetam and feels nauseous occasionally.

Fig. 1
figure 1

Axial magnetic resonance imaging (MRI) findings of patient 1 and patient 2 in fluid-attenuated inversion recovery (FLAIR) sequence. a Patient 1, a 35-year-old Chinese female, was diagnosed with typical limbic encephalitis, and MRI showed increased FLAIR signals of the right medial temporal lobe, including the amygdala and hippocampus. bd Patient 2, a 35-year-old male, was characterized by stiff-person syndrome, cerebellar ataxia and intractable epilepsy. MRI indicated postoperative changes in the left frontal lobe, volume reduction in the left temporal lobe and hippocampus, and encephalatrophy, especially in the bilateral cerebellum

Fig. 2
figure 2

Interictal phase electroencephalogram of patient 1. There were frequent sharp waves and slow waves in the right temporal regions in the interictal phase

Fig. 3
figure 3

Ictal phase electroencephalogram of patient 1. One clinical seizure was detected, which presented as a nauseous feeling and oropharyngeal automatism with impaired awareness, and the seizure lasted approximately 50 s second before sudden movement interruption of the patient, EEG showed widespread low voltage in the right leads, followed by rapid rhythmic changes with low amplitude in the right middle-posterior temporal region initially, and spreading to the right middle temporal region 2 s later. The amplitude increased and the frequency decreased gradually and then spread to adjacent leads, accompanied by electromyogram interference and motion artifacts

Patient 2: SPS, CA and intractable epilepsy

The patient is a 35-year-old male. His mother had suffered hyperthyroidism. He was also diagnosed with hyperthyroidism at 15 years old and developed nocturnal GTCSs 2 years later. These attacks lasted 4–5 min with a frequency of twice per month. At 24 years of age, he gradually developed barylalia and gait instability, and these symptoms progressively deteriorated. The second form of seizure occurred at 25 years of age and manifested as right-side deflection of the mouth, eyeball and head in an unconscious state, accompanied by oropharyngeal automatism and groping action. The seizure lasted 2–3 min and also occurred at night, with 1–2 seizures/year. Three years later, the frequency of the second type increased to once per week. He was diagnosed with late-onset type one diabetes at the age of 31, and blood sugar was well controlled. Three years ago, he underwent an excision of the epileptogenic focus in the left frontal lobe due to intractable epilepsy. After the operation, he received adequate doses of levetiracetam, clonazepam and carbamazepine, and although the first type of seizure disappeared, no improvement was achieved in the second type. Two months ago, the patient appeared to have a third form of seizure, which was characterized as a sudden trance and interruption of actions, lasting 2–20 min, with a frequency of one per day. In recent days, he suffered paroxysmal muscle stiffness and limb rigidity, accompanied by soreness of the lumbar muscles and difficulty in lying flat; these symptoms were prone to occur when stimulated by environmental factors. In addition, he occasionally developed palpitations, sweats and breathing difficulties and became irritable and depressed.

On neurological examination, he exhibited bilateral exophthalmos, dysarthria and left blepharospasm. The muscular tension and tendon reflex of the four limbs were decreased. He could not stand still and complete the finger-nose tests. The MoCA score was 22 (undergraduate degree). Routine blood examinations suggested that he had multiple kinds of autoimmune Abs simultaneously (seen in Table 1). Routine CSF analysis was normal. The onconeural and neuronal surface Abs test showed positive anti-GAD65 Abs in both serum and CSF. The brain MRI indicated postoperative changes and encephalatrophy (Fig. 1b–d). Electromyography showed continuous release of motor unit action potentials of the paraspinal muscles and rectus abdominis in a quiet state, which were obviously inhibited after intravenous diazepam, supporting the diagnosis of SPS.

A 5-day course of IVIG, 400 mg/kg/day, was initiated; subsequently, he took mycophenolate mofetil (gradually increased to 1500 mg/q12h), levetiracetam (250 mg/q12h), clonazepam (1 mg/q12h) and carbamazepine (200 mg/q12h) for long-term maintenance therapy. The above symptoms improved significantly. One month later, he received another 5-day course of IVIG for consolidation therapy. Three months later, the SPS had almost disappeared, the ataxia improved, he could walk by himself, and the number of seizures was reduced by more than 50%.

Patients from previous literatures

Seventy references were identified, and the included patients were all diagnosed with anti-GAD65 AE. In Table 2, we present the gathered data of all patients with different clinical manifestations and found that the effectiveness of IVMP was 45.56% (41/90) and that of IVIG was 36.71% (29/79). There was no statistically significant difference between IVMP and IVIG. In addition, 35 patients received IVMP and IVIG combination therapy, but only 42.86% (15/35) experienced an improvement in their clinical symptoms.

Table 2 IVMP and IVIG effectiveness of total patients in the previous 70 references

We further divided patients according to their typical clinical manifestations. In Table 3, we present data on the patients who manifested seizures, 42.65% (29/68) of whom were responsive to IVMP and 32.69% (17/52) responded to IVIG (in comparison with Table 2, in Table 3, we added an extra patient, whose seizures were not improved with IVMP, while IVMP was beneficial for CA) [28]. Among patients with SPS, 60.00% (3/5) benefited from IVMP, and 77.78% (7/9) gained amelioration from IVIG. In patients with CA, the effectiveness of IVMP and IVIG was 28.57% (2/7) and 55.56% (5/9), respectively (in Table 3, an extra patient was added in comparison with Table 2 [28]). In these above patients from different subgroups, there was no difference in therapeutic effect between the two kinds of treatment. Furthermore, in these AE patients who manifested seizures or CA, the effectiveness of combination therapy was 44.83% (13/29) and 25.00% (1/4), respectively. In our analysis, only one SPS patient received combination therapy but acquired no benefits [7].

Table 3 IVMP and IVIG effectiveness of patients with different clinical manifestations in the previous 70 references

As shown in Table 3 and Additional file 2: Table S1, of the 29 anti-GAD65 AE patients with tumors, 16 (55.17%) were male, and the median age was 61.8 years (IQR: 55.0–73.0 years). It should be pointed out that a 5-year-old girl was diagnosed with AE 13 months after hematopoietic stem cell transplantation (because of pineoblastoma); thus, the tumor and the AE may not have been directly related [36]. The median age of the remaining 28 patients increased to 63.9 years (IQR: 57.0–73.0 years), much higher than the median age of 48 years for the 106 anti-GAD65 patients without tumors in a previous study [7]. The most common symptom was SPS (11/29, 37.93%), followed by LE and nonlimbic AE (8/29 and 4/29, respectively). Among these patients, the therapeutic effect was dismal, with 29.41% and 42.11% improvement rates in symptoms for IVMP and IVIG, respectively, and no significant difference was found. Moreover, the combination therapy was effective for only 20.00% (2/10) of patients. In addition, 21 patients (72.41%) had oncological treatment (surgery, chemotherapy, and radiotherapy alone or combined). Other clinical data are listed in Additional file 2: Table S1.

It should be mentioned that we cannot compare the efficiency of combination therapy with monotherapy because of their statistical overlaps.


Screening for anti-GAD65 Abs has been widely reported among type 1 diabetic patients. However, increased awareness of neurologists in considering patients with anti-GAD65 Abs remains challenging. Among adult epilepsy patients with unknown etiology, three recent studies found that 1.7% (7/416) [37], 16.1% (18/112) [38], and 21.7% (5/23) of patients were positive for serum anti-GAD65 Abs [39]. The anti-GAD65-Ab positive rate was 17.0% (9/53) in LE patients [40]. Hence, the incidence of anti-GAD65 diseases may be seriously underestimated in practice. Here, we have described the clinical information of six anti-GAD65 AE patients in our cohort, five of whom were diagnosed after years of repeated medical consultations. Therefore, it should be noted that the time from symptom onset to immunotherapy was generally very long due to the lack of awareness, and some patients, including one of ours, even underwent brain tissue resection for drug-resistant epilepsy, which usually led to unsatisfactory outcomes [11, 41, 42].

Currently, there is no consensus on the treatment of anti-GAD65 AE. However, we believe that anti-GAD65 Abs are unique compared with other onconeural Abs. Patients with neurological syndromes associated with anti-GAD65 Abs are not typically considered at very high risk for cancer [7], and their prognosis seems to be very different from that of other traditional onconeural Abs [6, 25]. Jones et al. proved that the detection of anti-GAD65 Abs in adult autoimmune CA patients may predict relatively better immunotherapy response and neurological outcomes, similar to those of patients seropositive for neuronal surface Abs [6]. In contrast, the beneficial effects of immunotherapy on seizure frequency and cognition can be acquired only in paraneoplastic Ab-positive LE patients but not obviously in anti-GAD65 LE patients [25]. Furthermore, according to considerable research efforts devoted to the comparison of AE patients with anti-GAD65 Abs and those with anti-voltage gated potassium channel Abs, researchers have verified that the former tend to be seriously resistant to AEDs and immunotherapy, resulting in a chronic situation [12, 40, 43].

Gagnon et al. compiled an excellent review of 31 articles, describing the detailed treatment regimens of patients [8]. In contrast, our study considered only the response of patients to IVMP or IVIG due to the complexity of clinical treatments, and we attempted to analyze the effectiveness of the chosen treatments for patients from 70 previous articles. In patients who received IVMP, the effective rate was 45.56% (41/90), which was slightly higher than that of 36.71% (29/79) for patients who received IVIG. In the study of Gagnon et al. [8], the favorable outcome rates were 46.15% (6/13) for IVMP and 50.00% (2/4) for IVIG, and the effective rate for IVMP was similar to ours; however, the number of patients in our study was much more than that in their study because we searched more comprehensive references and included more patients. In patients with seizures, the response rates of IVMP and IVIG were 42.65% (29/68) and 32.69% (17/52), respectively, which were similar when counting all patients. Although we cannot recommend which method is more effective because no significant difference between IVMP and IVIG was found, importantly, it is very difficult for these patients to become seizure free from only AEDs [12, 13], and immunotherapy is currently still indispensable. In patients with SPS or CA, IVIG seemed to have a slightly better therapeutic effect than IVMP (77.78% vs. 60.00%; 55.56% vs. 28.57%). Consistent with our results, in a randomized, double-blinded, placebo-controlled crossover trial in anti-GAD65-Ab-positive SPS patients, IVIG resulted in significant improvements in objective stiffness parameters and activities of daily living [44], and corticosteroids or other therapies may be very disappointing [45]. However, as for CA, a study of 118 CA patients (including 41 anti-GAD65-Ab-positive cases) drew a different conclusion from ours, suggesting that corticosteroids may be the best regimen [6]. Considering the small sample size and the heterogeneity of subjects, further research is needed. Theoretically, combined therapy should be better than monotherapy, but the effective rate was disappointing in our research, with only 15/35 (42.86%) in all patients and 13/29 (44.83%), 0/1 (0%), and 1/4 (25.00%) in epilepsy, SPS and CA patients, respectively. A similar conclusion was obtained in the study of Gagnon et al. where steroids plus IVIG or plasma exchange had beneficial effects on only 6/18 (33.33%) anti-GAD65 LE patients [8]. One important reason contributing to the results is that patients who received combined immunotherapy were usually in serious conditions. Although we cannot make comparisons with monotherapy due to statistical overlaps, the method is still highly recommendable and very promising.

In our study, we also observed 29 anti-GAD65 AE patients who had coexisting tumors; in general, their reactions to immunotherapy were unsatisfactory. Ten of them received combined treatment of IVMP and IVIG, but only two benefited. Among the remaining 19 patients, the effectiveness of IVMP and IVIG was 55.56% (5/9) and 72.73% (8/11), respectively (one patient received IVMP and IVIG successively, not in combination); however, when calculated together, the improvement rates of IVMP and IVIG slumped to 29.41% (5/17) and 42.11% (8/19), respectively. Interestingly, a good outcome may be predicted for patients who had coexisting thymoma; only one out of 8 did not respond to immunotherapy, but the symptoms could be relieved by benzodiazepines or baclofen [46]. In contrast, only two out of 11 lung cancer patients acquired temporary improvement with immunotherapy, and one died in a short period of time [47]. The results are consistent with a previous observation, which found that 46.67% (7/15) of anti-GAD65-associated paraneoplastic neurological syndrome patients acquired clinical improvement or stability under various immunotherapy treatments, and amelioration occurred in only 4 thymic tumor patients, while only 1/6 of lung cancer patients was stable, and the rest were all deteriorated [7]. In addition to the older age compared with tumor-negative anti-GAD65 AE patients, which probably led to poor prognosis, tumor treatment is also a factor that needs to be considered. Among patients undergoing active treatment of primary tumors, 13/21 (61.90%) gained benefits from immunotherapy, while no patients improved if the tumor was not managed. Considering the adverse influences of particular tumor types, old age and passive tumor treatment on prognosis, the underlying tumors should be screened in some conditions early [7], and elaborate studies involving various factors are critically needed to guide future therapy.

There was no denying that some anti-GAD65 patients completely recovered without immunotherapy; however, they were reported just in scattered case reports [12, 19, 23, 48, 49]. We firmly believe that this AE was characterized as a form of chronic, non-remitting disorder in most cases, which was consistent with the standpoint from other researchers [8, 11, 40]. Several medical centers also tried to summarize their own treatment experiences and expected to offer the best treatment plan, but all ended in failure [9, 11, 28, 40, 48]. Seven anti-GAD65 LE patients received monthly high-intensity immunotherapy, with a median total methylprednisolone equivalent dose up to 18 g, but none became seizure free [40]. Malter et al. retrospectively analyzed the complex treatment regimens of 13 anti-GAD65 epilepsy patients and found that the seizure and cognitive response to immunotherapies were poor, with the most frequently achieved seizure response (≥ 50% reduction) occurring in only 45% of patients under intensive corticosteroid treatment (median 19 g methylprednisolone equivalent) [11]. Pittock et al. found that the benefits from a trial of immunotherapies in 27 patients with anti-GAD65 Abs were temporary [9]. Identical conclusions were obtained in 12 other patients [28, 48], making the routine use of immunotherapy debatable. Nevertheless, anti-GAD65-associated SPS is likely to be an exception because more than 70% of patients can obtain relief from IVIG, and notably, the abovementioned clinical trial further clarifies the validity of IVIG [44].

Our study also had some limitations. First, the retrospective analysis of the literature had the following inherent limitations: the diverse vocabularies could have led to a certain degree of misinterpretation; the description of clinical features, investigations, and outcomes also differed from one article to another. Second, we abandoned some related literature because we could not obtain the prognosis after immunotherapy, leading to statistical bias. Third, different hospitals had their own experience in choosing IVMP, IVIG or others, and the absence of IVMP or IVIG therapy did not mean that the patient was insensitive to it. Fourth, we excluded many patients who received combination therapy (IVMP/IVIG and others) because we could not judge which drug had an effect. Fifth, there was a certain ineluctable subjectivity in our judgment of reactivity to therapy. Sixth, there were significant differences in the follow-up time of patients, and we focused only on whether the clinical symptoms were improved after immunotherapy, leading to the inability to judge the effective time of IVIG and IVMP treatment. Although many patients were initially sensitive to immunotherapy, they will relapse inevitably after a period of time [13, 24, 36, 41, 48, 50,51,52,53,54], which we considered may be a short-term outcome. Considering the shortcomings of our research and limitations in this field, therapeutic strategies cannot be reasonably recommended currently except IVIG for anti-GAD65-associated SPS, and long-term immunotherapy may be the best option.


Here, we reported six anti-GAD65 AE patients and found that they all achieved clinical improvements in a short period of time after immunotherapy. However, by summarizing the therapeutic effects of previous patients, we have confirmed that this AE usually has a poor response to IVMP or IVIG, except anti-GAD65-associated SPS. Larger prospective studies enrolling large numbers of patients are required to identify the optimal therapeutic strategy.

Availability of data and materials

The datasets supporting the conclusions of this article are available when contacting authors.



Glutamic acid decarboxylase 65




Cerebrospinal fluid


Stiff-person syndrome


Cerebellar ataxia


Limbic encephalitis


Autoimmune encephalitis


Intravenous methylprednisolone


Intravenous immunoglobulin


Antiepileptic drugs


Generalized tonic–clonic seizures


Montreal Cognitive Assessment


Magnetic resonance imaging




  1. Daif A, Lukas RV, Issa NP, Javed A, VanHaerents S, Reder AT, Tao JX, Warnke P, Rose S, Towle VL, et al. Antiglutamic acid decarboxylase 65 (GAD65) antibody-associated epilepsy. Epilepsy Behav. 2018;80:331–6.

    Article  PubMed  Google Scholar 

  2. Dubey D, Pittock SJ, Kelly CR, McKeon A, Lopez-Chiriboga AS, Lennon VA, Gadoth A, Smith CY, Bryant SC, Klein CJ, et al. Autoimmune encephalitis epidemiology and a comparison to infectious encephalitis. Ann Neurol. 2018;83(1):166–77.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  3. Hackert JK, Müller L, Rohde M, Bien CG, Köhling R, Kirschstein T. Anti-GAD65 containing cerebrospinal fluid does not alter GABAergic transmission. Front Cell Neurosci. 2016;10:130.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  4. Gresa-Arribas N, Ariño H, Martínez-Hernández E, Petit-Pedrol M, Sabater L, Saiz A, Dalmau J, Graus F. Antibodies to inhibitory synaptic proteins in neurological syndromes associated with glutamic acid decarboxylase autoimmunity. PLoS ONE. 2015;10(3):e0121364.

    Article  PubMed Central  CAS  PubMed  Google Scholar 

  5. McKeon A, Tracy JA. GAD65 neurological autoimmunity. Muscle Nerve. 2017;56(1):15–27.

    Article  CAS  PubMed  Google Scholar 

  6. Jones AL, Flanagan EP, Pittock SJ, Mandrekar JN, Eggers SD, Ahlskog JE, McKeon A. Responses to and outcomes of treatment of autoimmune cerebellar ataxia in adults. JAMA Neurol. 2015;72(11):1304–12.

    Article  PubMed  Google Scholar 

  7. Ariño H, Höftberger R, Gresa-Arribas N, Martínez-Hernández E, Armangue T, Kruer MC, Arpa J, Domingo J, Rojc B, Bataller L, et al. Paraneoplastic neurological syndromes and glutamic acid decarboxylase antibodies. JAMA Neurol. 2015;72(8):874–81.

    Article  PubMed Central  PubMed  Google Scholar 

  8. Gagnon MM, Savard M. Limbic encephalitis associated with GAD65 antibodies: brief review of the relevant literature. Can J Neurol Sci. 2016;43(4):486–93.

    Article  PubMed  Google Scholar 

  9. Pittock SJ, Yoshikawa H, Ahlskog JE, Tisch SH, Benarroch EE, Kryzer TJ, Lennon VA. Glutamic acid decarboxylase autoimmunity with brainstem, extrapyramidal, and spinal cord dysfunction. Mayo Clin Proc. 2006;81(9):1207–14.

    Article  CAS  PubMed  Google Scholar 

  10. Korff CM, Parvex P, Cimasoni L, Wilhelm-Bals A, Hampe CS, Schwitzgebel VM, Michel M, Siegrist CA, Lalive PH, Seeck M. Encephalitis associated with glutamic acid decarboxylase autoantibodies in a child: a treatable condition. Arch Neurol. 2011;68(8):1065–8.

    Article  PubMed  Google Scholar 

  11. Malter MP, Frisch C, Zeitler H, Surges R, Urbach H, Helmstaedter C, Elger CE, Bien CG. Treatment of immune-mediated temporal lobe epilepsy with GAD antibodies. Seizure. 2015;30:57–63.

    Article  CAS  PubMed  Google Scholar 

  12. Feyissa AM, López Chiriboga AS, Britton JW. Antiepileptic drug therapy in patients with autoimmune epilepsy. Neurol Neuroimmunol Neuroinflamm. 2017;4(4):e353.

    Article  PubMed Central  PubMed  Google Scholar 

  13. Farooqi MS, Lai Y, Lancaster E, Schmitt SE, Sachais BS. Therapeutic plasma exchange and immunosuppressive therapy in a patient with anti-GAD antibody-related epilepsy: quantification of the antibody response. J Clin Apher. 2015;30(1):8–14.

    Article  PubMed  Google Scholar 

  14. Olson JA, Olson DM, Sandborg C, Alexander S, Buckingham B. Type 1 diabetes mellitus and epilepsia partialis continua in a 6-year-old boy with elevated anti-GAD65 antibodies. Pediatrics. 2002;109(3):E50.

    Article  PubMed  Google Scholar 

  15. Sinnreich M, Assal F, Hefft S, Magistris MR, Chizzolini C, Landis T, Burkhard PR. Anti-GAD antibodies and breast cancer in a patient with stiff-person syndrome: a puzzling association. Eur Neurol. 2001;46(1):51–2.

    Article  CAS  PubMed  Google Scholar 

  16. Liu B, Zhou Y, Meng L, Skinner H. A survival case of super-refractory status epilepticus due to glutamic acid decarboxylase antibodies-associated limbic encephalitis. Cureus. 2018;10(8):e3125.

    PubMed Central  PubMed  Google Scholar 

  17. Hagiwara H, Enomoto-Nakatani S, Sakai K, Ugawa Y, Kusunoki S, Kanazawa I. Stiff-person syndrome associated with invasive thymoma: a case report. J Neurol Sci. 2001;193(1):59–62.

    Article  CAS  PubMed  Google Scholar 

  18. Sharma CM, Pandey RK, Kumawat BL, Khandelwal D, Gandhi P. A unique combination of autoimmune limbic encephalitis, type 1 diabetes, and Stiff person syndrome associated with GAD-65 antibody. Ann Indian Acad Neurol. 2016;19(1):146–9.

    Article  PubMed Central  PubMed  Google Scholar 

  19. Petit-Pedrol M, Armangue T, Peng X, Bataller L, Cellucci T, Davis R, McCracken L, Martinez-Hernandez E, Mason WP, Kruer MC, et al. Encephalitis with refractory seizures, status epilepticus, and antibodies to the GABAA receptor: a case series, characterisation of the antigen, and analysis of the effects of antibodies. Lancet Neurol. 2014;13(3):276–86.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  20. Quek AM, Britton JW, McKeon A, So E, Lennon VA, Shin C, Klein C, Watson RE, Kotsenas AL, Lagerlund TD, et al. Autoimmune epilepsy: clinical characteristics and response to immunotherapy. Arch Neurol. 2012;69(5):582–93.

    Article  PubMed Central  PubMed  Google Scholar 

  21. Rakocevic G, Hussain A. Stiff person syndrome improvement with chemotherapy in a patient with cutaneous T cell lymphoma. Muscle Nerve. 2013;47(6):938–9.

    Article  PubMed  Google Scholar 

  22. Ances BM, Vitaliani R, Taylor RA, Liebeskind DS, Voloschin A, Houghton DJ, Galetta SL, Dichter M, Alavi A, Rosenfeld MR, et al. Treatment-responsive limbic encephalitis identified by neuropil antibodies: MRI and PET correlates. Brain. 2005;128(Pt 8):1764–77.

    Article  PubMed  Google Scholar 

  23. Haberlandt E, Bast T, Ebner A, Holthausen H, Kluger G, Kravljanac R, Kröll-Seger J, Kurlemann G, Makowski C, Rostasy K, et al. Limbic encephalitis in children and adolescents. Arch Dis Child. 2011;96(2):186–91.

    Article  CAS  PubMed  Google Scholar 

  24. Kojima G, Inaba M, Bruno MK. PET-positive extralimbic presentation of anti-glutamic acid decarboxylase antibody-associated encephalitis. Epileptic Disord. 2014;16(3):358–61.

    Article  PubMed  Google Scholar 

  25. Hansen N, Widman G, Witt JA, Wagner J, Becker AJ, Elger CE, Helmstaedter C. Seizure control and cognitive improvement via immunotherapy in late onset epilepsy patients with paraneoplastic versus GAD65 autoantibody-associated limbic encephalitis. Epilepsy Behav. 2016;65:18–24.

    Article  CAS  PubMed  Google Scholar 

  26. Ben Achour N, Ben Younes T, Rebai I, Ben Ahmed M, Kraoua I, Ben Youssef-Turki I. Severe dysautonomia as a main feature of anti-GAD encephalitis: report of a paediatric case and literature review. Eur J Paediatr Neurol. 2018;22(3):548–51.

    Article  PubMed  Google Scholar 

  27. Finelli PF. Autoimmune limbic encephalitis with GAD antibodies. Neurohospitalist. 2011;1(4):178–81.

    Article  PubMed Central  PubMed  Google Scholar 

  28. Lilleker JB, Biswas V, Mohanraj R. Glutamic acid decarboxylase (GAD) antibodies in epilepsy: diagnostic yield and therapeutic implications. Seizure. 2014;23(8):598–602.

    Article  PubMed  Google Scholar 

  29. Akaishi T, Jin K, Kato K, Itabashi H, Misu T, Tateyama M, Iwasaki M, Aoki M, Nakasato N. Clinical characteristics of four patients with temporal lobe epilepsy associated with elevated anti-GAD antibodies. Rinsho Shinkeigaku. 2015;55(11):804–9.

    Article  PubMed  Google Scholar 

  30. Specchio N, Fusco L, Claps D, Vigevano F. Epileptic encephalopathy in children possibly related to immune-mediated pathogenesis. Brain Dev. 2010;32(1):51–6.

    Article  PubMed  Google Scholar 

  31. Knudsen A, Bredholt G, Storstein A, Oltedal L, Davanger S, Krossnes B, Honnorat J, Vedeler CA. Antibodies to CRMP3-4 associated with limbic encephalitis and thymoma. Clin Exp Immunol. 2007;149(1):16–22.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  32. Silverman IE. Paraneoplastic stiff limb syndrome. J Neurol Neurosurg Psychiatry. 1999;67(1):126–7.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  33. Agarwal PA, Ichaporia NR. Glutamic acid decarboxylase antibody-positive paraneoplastic stiff limb syndrome associated with carcinoma of the breast. Neurol India. 2010;58(3):449–51.

    Article  PubMed  Google Scholar 

  34. Ganor Y, Goldberg-Stern H, Amrom D, Lerman-Sagie T, Teichberg VI, Pelled D, Futerman AH, Zeev BB, Freilinger M, Verheulpen D, et al. Autoimmune epilepsy: some epilepsy patients harbor autoantibodies to glutamate receptors and dsDNA on both sides of the blood-brain barrier, which may kill neurons and decrease in brain fluids after hemispherotomy. Clin Dev Immunol. 2004;11(3–4):241–52.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  35. Ganor Y, Freilinger M, Dulac O, Levite M. Monozygotic twins discordant for epilepsy differ in the levels of potentially pathogenic autoantibodies and cytokines. Autoimmunity. 2005;38(2):139–50.

    Article  CAS  PubMed  Google Scholar 

  36. Nagai K, Maekawa T, Terashima H, Kubota M, Ishiguro A. Severe anti-GAD antibody-associated encephalitis after stem cell transplantation. Brain Dev. 2019;41(3):301–4.

    Article  PubMed  Google Scholar 

  37. Brenner T, Sills GJ, Hart Y, Howell S, Waters P, Brodie MJ, Vincent A, Lang B. Prevalence of neurologic autoantibodies in cohorts of patients with new and established epilepsy. Epilepsia. 2013;54(6):1028–35.

    Article  PubMed  Google Scholar 

  38. Dubey D, Alqallaf A, Hays R, Freeman M, Chen K, Ding K, Agostini M, Vernino S. Neurological autoantibody prevalence in epilepsy of unknown etiology. JAMA Neurol. 2017;74(4):397–402.

    Article  PubMed  Google Scholar 

  39. Falip M, Carreño M, Miró J, Saiz A, Villanueva V, Quílez A, Molins A, Barceló I, Sierra A, Graus F. Prevalence and immunological spectrum of temporal lobe epilepsy with glutamic acid decarboxylase antibodies. Eur J Neurol. 2012;19(6):827–33.

    Article  CAS  PubMed  Google Scholar 

  40. Malter MP, Helmstaedter C, Urbach H, Vincent A, Bien CG. Antibodies to glutamic acid decarboxylase define a form of limbic encephalitis. Ann Neurol. 2010;67(4):470–8.

    Article  PubMed  Google Scholar 

  41. Hansen N, Ernst L, Rüber T, Widman G, Becker AJ, Elger CE, Helmstaedter C. Pre- and long-term postoperative courses of hippocampus-associated memory impairment in epilepsy patients with antibody-associated limbic encephalitis and selective amygdalohippocampectomy. Epilepsy Behav. 2018;79:93–9.

    Article  PubMed  Google Scholar 

  42. Bonello M, Larner AJ, Marson AG. Profound amnesia after temporal lobectomy: an autoimmune process resembling patient hm. Case Rep Neurol. 2014;6(3):251–5.

    Article  PubMed Central  PubMed  Google Scholar 

  43. Frisch C, Malter MP, Elger CE, Helmstaedter C. Neuropsychological course of voltage-gated potassium channel and glutamic acid decarboxylase antibody related limbic encephalitis. Eur J Neurol. 2013;20(9):1297–304.

    Article  CAS  PubMed  Google Scholar 

  44. Dalakas MC, Fujii M, Li M, Lutfi B, Kyhos J, McElroy B. High-dose intravenous immune globulin for stiff-person syndrome. N Engl J Med. 2001;345(26):1870–6.

    Article  CAS  PubMed  Google Scholar 

  45. Alexopoulos H, Dalakas MC. Immunology of stiff person syndrome and other GAD-associated neurological disorders. Expert Rev Clin Immunol. 2013;9(11):1043–53.

    Article  CAS  PubMed  Google Scholar 

  46. Thomas S, Critchley P, Lawden M, Farooq S, Thomas A, Proudlock FA, Constantinescu CS, Gottlob I. Stiff person syndrome with eye movement abnormality, myasthenia gravis, and thymoma. J Neurol Neurosurg Psychiatry. 2005;76(1):141–2.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  47. Spitz M, Ferraz HB, Barsottini OG, Gabbai AA. Progressive encephalomyelitis with rigidity: a paraneoplastic presentation of oat cell carcinoma of the lung. Case report. Arq Neuropsiquiatr. 2004;62(2B):547–9.

    Article  PubMed  Google Scholar 

  48. Mäkelä KM, Hietaharju A, Brander A, Peltola J. Clinical management of epilepsy with glutamic acid decarboxylase antibody positivity: the interplay between immunotherapy and anti-epileptic drugs. Front Neurol. 2018;9:579.

    Article  PubMed Central  PubMed  Google Scholar 

  49. Graus F, Saiz A, Lai M, Bruna J, López F, Sabater L, Blanco Y, Rey MJ, Ribalta T, Dalmau J. Neuronal surface antigen antibodies in limbic encephalitis: clinical-immunologic associations. Neurology. 2008;71(12):930–6.

    Article  CAS  PubMed Central  PubMed  Google Scholar 

  50. Akman CI, Patterson MC, Rubinstein A, Herzog R. Limbic encephalitis associated with anti-GAD antibody and common variable immune deficiency. Dev Med Child Neurol. 2009;51(7):563–7.

    Article  PubMed  Google Scholar 

  51. Grilo E, Pinto J, Caetano JS, Pereira H, Cardoso P, Cardoso R, Dinis I, Pereira C, Fineza I, Mirante A. Type 1 diabetes and GAD65 limbic encephalitis: a case report of a 10-year-old girl. J Pediatr Endocrinol Metab. 2016;29(8):985–90.

    Article  PubMed  Google Scholar 

  52. García García ME, Castrillo SM, Morales IG, Di Capua Sacoto D, Dolado AM. Acute amnesia and seizures in a young female. Epileptic Disord. 2013;15(4):455–60.

    PubMed  Google Scholar 

  53. Georgieva Z, Parton M. Cerebellar ataxia and epilepsy with anti-GAD antibodies: treatment with IVIG and plasmapheresis. BMJ Case Rep. 2014;2014:2013202314.

    Article  Google Scholar 

  54. Markakis I, Alexopoulos H, Poulopoulou C, Akrivou S, Papathanasiou A, Katsiva V, Lyrakos G, Gekas G, Dalakas MC. Immunotherapy-responsive limbic encephalitis with antibodies to glutamic acid decarboxylase. J Neurol Sci. 2014;343(1–2):192–4.

    Article  CAS  PubMed  Google Scholar 

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This work was supported by grants from the Application Research of Capital Clinical Characteristics (No. Z181100001718082), the Beijing Dongcheng District Outstanding Talent Funding Project (No. 2019DCT-M-18), the National Natural Science Foundation of China (No. 81301119), the Beijing Municipal Administration of Hospitals’ Youth Programme (No. QML20160504), the China Association Against Epilepsy-UCB Research Fund (No. CAAE- UCB2016008) and the Natural Science Foundation of Capital Medical University (No. PYZ2017055). The funding bodies had no influence on the design of the study, collection, analysis, and interpretation of data or writing of the manuscript.

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RJL, YDZ, QW, XQS and ZML provided the clinical data. TRL and RJL acquired the literature data. TRL drafted the manuscript. RJL critically revised the manuscript for important intellectual content. All authors read and approved the final manuscript.

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Correspondence to Rui-Juan Lv.

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The study was approved by the Medical Ethics Committee of Beijing Tiantan Hospital, Capital Medical University, and was carried out in accordance with the Declaration of Helsinki. We confirm that we have read the Journal’s position on issues involved in ethical publication and affirm that this report is consistent with those guidelines. All participants gave written informed consent to participate.

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All participants provided written consent for publication for the publication of clinical details.

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Supplementary information

Additional file 1.

References included in the statistics. We conducted a search on PubMed for articles up to April 2019 and finally included 70 references. The article type, clinical characteristics of each patient, and whether the patient received combined therapy are noted.

Additional file 2: Tabe S1.

Clinical data of 29 patients who had coexisting tumors in previous references. The clinical information, concomitant tumor, tumor therapy and response to immunotherapy of each patient are listed.

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Li, TR., Zhang, YD., Wang, Q. et al. Intravenous methylprednisolone or immunoglobulin for anti-glutamic acid decarboxylase 65 antibody autoimmune encephalitis: which is better?. BMC Neurosci 21, 13 (2020).

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