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Fig. 10 | BMC Neuroscience

Fig. 10

From: Identification of the neurotransmitter profile of AmFoxP expressing neurons in the honeybee brain using double-label in situ hybridization

Fig. 10

Cluster medioventral to the lobula-posterior part (mvLO-p). a Honeybee brain schematic drawing focuses on a posterior brain hemisphere. AmFoxP neurons are indicated by small empty circles within the boxed area which indicates localization of panels (bj). b Confocal image (2 µm confocal section) of a forager brain stained with the AmFoxP42kDa antiserum (white label) shows the mvLO-p. cl dISH stainings showing AmFoxP transcript (magenta), neurotransmitter transcript (cyan) and DAPI-stained nuclei (blue) on 12 µm cryosections. c Cryosection of a newly emerged individual. d Cryosection of a 4 days old individual from the ‘honeycomb’ group. e Cryosection of a 11 days old individual of the ‘honeycomb’ group. f Cryosection of a 26 days old individual of the ‘mini-cage’ group. gj Adjacent cryosections of a 15 days old individual from the ‘unmanipulated’ group. k Cryosection of a 31 days old individual of the ‘incubator’ group. l Cryosection of a 50 days old individual of the ‘unmanipulated’ group. Inset in c, g, l show magnification of boxed area with co-localization of the two probes. For explanations of pictograms see Table 1, for anatomical abbreviations see Table 5. Scale bar b: 50 µm, as orientation for all other panels

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