- Research Article
- Open Access
CX3C chemokine receptor 1 deficiency modulates microglia morphology but does not affect lesion size and short-term deficits after experimental stroke
- Gerlinde van der Maten†1,
- Vivien Henck†1,
- Tadeusz Wieloch1 and
- Karsten Ruscher1Email authorView ORCID ID profile
© The Author(s) 2017
- Received: 8 November 2016
- Accepted: 14 December 2016
- Published: 6 January 2017
The fractalkine/CX3C chemokine receptor 1 (CX3CR1) pathway has been identified to play an essential role in the chemotaxis of microglia, leukocyte trafficking and microglia/macrophage recruitment. It has also been shown to be important in the regulation of the inflammatory response in the early phase after experimental stroke. The present study was performed to investigate if CX3CR1 deficiency affects microglia during the first 14 days with consequences for tissue damage after experimental stroke.
CX3CR1 deficiency significantly increased the number of intersections of GFP positive microglia in the proximal peri-infarct area at 2, 7 and 14 days following tMCAO compared to heterozygous and wildtype littermates. In addition, the length of microglial branches increased until day 7 in CX3CR1 knockout mice while the presence of a functional CX3CR1 allele resulted in a gradual reduction of their length following tMCAO. After stroke, wildtype, heterozygous and CX3CR1 deficient mice did not show differences in the composite neuroscore and assessment of infarct volumes from CX3CR1 wildtype, heterozygous and deficient mice revealed no differences between the genotypes 7 and 14 days after stroke.
Results demonstrate that CX3CR1 deficiency affects the morphology of GFP positive microglia located in the proximal peri-infarct region during the first 14 days after tMCAO. Our data also indicate that CX3CR1 deficiency does not affect definite infarct volumes. Modulation of the CX3CR1 pathway may have implication for microglia function contributing to mechanisms of tissue reorganization in the post-ischemic brain.
- Green Fluorescent Protein
- Infarct Volume
- Normal Donkey Serum
- Sholl Analysis
- Microglia Morphology
For development of new therapeutics in stroke, it is important to understand mechanisms of tissue reorganisation in the brain after ischemia. Based on recent studies it is clear that parallel and partially opposite processes contribute to restoration of brain function. The interaction of multiple mechanisms including neuronal plasticity, astrogliosis, cell- and angiogenesis and inflammation are important for the final outcome after stroke.
During the recovery process, the release of pro-inflammatory molecules can initiate and perpetuate the local inflammatory response in the postischemic brain . Examples of these molecules are free DNA, membrane compounds, oxidized lipids and free radicals, and they can be referred to as danger associated molecules (DAMPs). DAMPs can bind to receptors on brain resident microglia and astrocytes . Cytokine and chemokine release increases, as does the expression of adhesion molecules on brain resident cells and in particular endothelial cells. Circulating immune cells bind to these molecules and migrate into the ischemic territory. These cells also release neurotoxic cytokines, chemokines and free radicals, which may exacerbate the inflammatory process [3, 4]. On the other hand, inflammatory processes contribute to tissue repair. Macrophages clear up the necrotic debris, and immune cells as well as pro-inflammatory cytokines are involved in facilitating neuronal plasticity .
Accumulation of immune cells is a well-regulated process and even weeks after stroke, immune cells can be detected in the ischemic territory . Microglial cells are resident macrophages of the brain, and play a major role in post-injury inflammation. In the healthy brain they survey the environment, and the cells are characterized by a ramified appearance [4, 7]. In different brain pathologies, however, microglial cells transform associated with the expression of different cell surface receptors, production of immune mediators, and the capacity to phagocytise. In this state, they are indistinguishable from recruited peripheral macrophages. Previous studies have demonstrated that after stroke microglia adapt a pro-inflammatory state and contribute to post-ischemic inflammation [4, 8, 9].
Fractalkine (CX3CL1) is a chemokine that, in the brain, is expressed mainly by neurons, although there are some studies indicating an expression by glial cells as well [10–14]. Fractalkine exists in a membrane-bound form and, after proteolytic cleavage by ADAM10 or ADAM17, in a soluble form [15, 16]. Membranous fractalkine is involved in mechanisms of cell adhesion, its soluble form can act as chemoattractant . The sole receptor for fractalkine is CX3C chemokine receptor 1 (CX3CR1) expressed on different immune cell populations [12, 17–19]. Within the first hours after ischemic injury, fractalkine expression is increased in necrotic neurons in the infarct core . During the first week after stroke, there is also a delayed upregulation of fractalkine in cells of the peri-infarct area . CX3CR1 positive cells can be detected in the ischemic tissue after 24 h, and the number of CX3CR1 positive microglia is strongly up-regulated during the first weeks after permanent focal ischemia .
The present investigation was conducted to study the effect of CX3CR1 deficiency on definitive tissue damage in the postischemic brain after tMCAO. Based on previous studies, we hypothesized that CX3CR1 deficiency may contribute to the morpholgy of microglia in the proximal peri-infarct area during the first 2 weeks with possible consequences for their function and contribution to recovery of lost neurological function after stroke.
For the present study transgenic male C57/B6 mice that carried green fluorescent protein (GFP) under control of the CX3CR1-promoter (CX3CR1GFP/GFP and CX3CR1GFP/+) and CX3CR1+/+ wildtype littermates were used (age between 10 and 16 weeks). While CX3CR1GFP/GFP animals do not express a functional receptor [referred to as knockout (ko) mice], CX3CR1GFP/+ mice [referred to as heterozygous (hz) mice] express GFP but still have one allele for a functional CX3CR1 receptor. Mice were bred as CX3CR1GFP/+ mice and offspring littermates have been used for experiments. As previously described  no phenotypic differences have been observed between the genotypes, body weights of wildtype (CX3CR1+/+) and deficient (CX3CR1GFP/GFP) mice did not differ before surgery (wt: 24.5 ± 3.4 g; ko: 25.9 ± 3.3 g) and on day 7 after tMCAO (wt: 21.4 ± 4.1 g; ko: 21.9 ± 2.6 g). All animal procedures included in this study were approved by the Malmö/Lund ethical committee. Experiments were carried out according the ARRIVE criteria to perform animal experiments and were blinded to the experimentators performing the surgeries.
Transient occlusion of the middle cerebral artery
Mice were anesthetized by inhalation of initially 1.5% isofluorane in O2:N2O (30:70) and sustained at 1.0% to 1.2% throughout the occlusion period (IsobaVet, Schering-Plough Animal Health, Milton Keynes, UK). Body temperature was kept at 37 °C. Regional cerebral blood flow (rCBF) was monitored through an optical fibre probe (Probe 318-I, Perimed, Järfälla, Sweden) fixed to the skull at 1 mm posterior and 3.5 mm lateral to Bregma and connected to a laser Doppler flow meter (Periflux System 5000, Perimed). A customized silicon rubber-coated monofilament (Doccol Corporation) size 6–0, diameter 0.09–0.11 mm, length 20 mm; diameter with coating 0.25 ± 0.02 mm to 0.27 ± 0.02 mm (adapted to the age and weight of the animal) was inserted into the common carotid artery and advanced into the internal carotid artery until the origin of the middle cerebral artery (MCA), given by a sudden drop in rCBF. After 45 min, the filament was withdrawn and reperfusion was observed. The animals were placed in a heating box at 37 °C overnight, to avoid post-surgical hypothermia. Physiological parameters body weight and body temperature have been monitored throughout the first week of the study. In order to analyze the inflammatory response after tMCAO, CX3CR1 heterozygous and knockout mice have been sacrificed on day 2, 7 or 14 after tMCAO. In total 67 mice (wt: 23; hz: 18; ko: 26) were operated. Out of those 26 mice (wt: 9; hz: 5; ko: 12) died during the first 4 days of the study and were not included in the analysis. Numbers of animals included in analyses are indicated in respective figure legends.
Neurological deficits were assessed by a composite neuroscore containing the following tests: (1) animal rotation (0–5 points; 0—barely able to move but if so, moving circularly, 1—big impairment, totally rotating over the body axis or with parallel dislocation, 2—big impairment, able to walk while circumscribing small circles, 3—rotated in medium to big sized circles, 4—rotates in big circles but is able to walk straight, 5—normal function); (2) paw placement test (0–5 points; 0—paw totally immobilized, hanging down, no movement, 1—paw hanging, but little movement back and forth (horizontal plane), 2—paw hanging, horizontal and vertical movement below table’s level, 3—paw hanging horizontal and vertical movement up to table’s surface level, 4—paw hanging, mobile in all directions, reaching the table’s surface, 5—normal, paw is immediately taken up to the table’s surface); (3) forelimb flexion on suspension test (0–4 points; 0—impaired paw is not moving and tightly held to the trunk, 1—impaired paw mostly held to the trunk, slightly movable, 2—impaired paw moves up and down, asymmetry to other paw, 3—slight asymmetry between the paws, both paws well movable, 4—normal, symmetric); (4) hind limb flexion response (0–4 points; 0—impaired paw is not moving and tightly held to the trunk, 1—impaired paw mostly held to the trunk, slightly movable, 2—impaired paw moves up and down, asymmetry to other paw, 3—slight asymmetry between the paws, both paws well movable, 4—normal, symmetric) and (5) lateral pulse test (0–4 points; 0—severely impaired and asymmetrical, 1—impaired, distinct asymmetry, 2—partially impaired, visible asymmetry in response to pulse, 3—slightly impaired, almost no asymmetry, 4—normal). Animals have been tested on day 1 after the surgery and the maximum score to achieve was 22 points .
Brain sections (thickness 30 µm) from 4% paraformaldehyde-perfused animals were rinsed in phosphate buffered saline (PBS). Endogenous peroxidase activity was quenched by washing the sections in a solution of PBS with 3% H2O2 and 10% methanol for 15 min. After subsequent rinsing in PBS, blocking was achieved by incubation in a solution containing 5% normal donkey serum (NDS) in 0.25% Triton X-100 in PBS (Tx/PBS) for 1 h. Thereafter, sections were incubated with a mouse anti neuronal nuclei (NeuN) antibody (diluted at 1:1000, Merck Millipore, Billerica, MA, USA) at 4 °C in 5% NDS in Tx/PBS overnight. The next day, sections were rinsed in 1% NDS in Tx/PBS followed by incubation with respective biotinylated secondary antibodies in 2% NDS in Tx/PBS at room temperature for 90 min. Sections were washed with Tx/PBS and incubated with avidin–biotin-complex (ABC) (Vector Laboratories, CA, USA) for 1 h. Following this incubation, the sections were rinsed in PBS. The ABC reaction was visualized using NiDAB (DAB (Dabsafe, Saveen Werner AB, Sweden)) with the addition of NiCl2 and a mixture of 3% H2O2 in H2O. Thereafter, sections were rinsed in PBS, mounted on glass slides, dried overnight, dehydrated, treated with xylene and then cover-slipped with Pertex. (Histolab AB, Sweden).
Brain sections (thickness 30 µm) from 4% paraformaldehyde-perfused animals were washed in PBS (3 × 10 min), blocking was achieved by 5% normal donkey serum in Tx/PBS for 60 min. The sections were then incubated with primary antibody (neuronal nuclei (NeuN), Merck Millipore, Europe; diluted at 1:1000 (ms antibody) or 1:5000 (rb antibody)) in Tx/PBS in 5% NDS at 4 °C overnight. The next day, the sections were rinsed with 1% NDS in Tx/PBS thrice for 10 min. All following steps have been performed in darkness in order to preserve the fluorophore conjugated to the secondary antibody. Sections were incubated with the secondary antibodies conjugated either with biotin or Cy3 (each diluted at 1:400) in 2% NDS in Tx/PBS for 90 min at room temperature and rinsed in PBS thrice for 10 min. Thereafter, sections incubated with biotinylated secondary antibody have been incubated with AF488 conjugated streptavidin (1:400) for 60 min at room temperature. Finally, the sections were rinsed in PBS thrice for 10 min and mounted on supercharged glass slides (Menzel, Braunschweig, Germany), allowed to dry, cover-slipped using PVA-DABCO (Saveen-Werner, Malmö, Sweden) and analysed using LSM 510 confocal microscopy (Zeiss Jena, Germany).
Infarct size measurements
Brains from perfusion fixed animals were cut into coronal sections with a thickness of 30 µm and stained for neuronal nuclei (NeuN) . The noninjured portion of the ipsilateral and contralateral hemisphere were encircled and the indirect infarct volume was calculated by integration of areas from serial sections of each brain as described previously .
Statistical analysis was performed using Excel and IBM-SPSS (Version 22, IBM, Stockholm, Sweden). Sholl analysis data are presented as medians with the 25th and 75th percentiles. Comparison between groups was performed using the Student’s t test for GFP positive cells and Kruskal–Wallis analysis for Iba-1 positive cells. Infarct volumes are presented as mean ± standard deviation (SD). Statistical analysis has been performed by one-way analysis of variance. No statistical difference has been reached at 7 days (p = 0.3049) and 14 days (p = 0.6128), posthoc corrections were not performed. Neuroscore data are presented as medians with the 25th and 75th percentiles and whiskers. Statistical analysis have been performed by the Kruskal–Wallis test (p = 0.8196).
Effect of CX3CR1 deficiency on the morphology of microglia after stroke
Impact of CX3CR1 deficiency on infarct volume and functional outcome after tMCAO
The present study was conducted to characterize the inflammatory response and in particular the relevance of CX3CR1 on microglia morpholgy and lesion size during the first 14 days after transient occlusion of the middle cerebral artery. We found that CX3CR1 deficiency significantly altered the microglial response in the proximal peri-infarct area. We also provide the evidence that CX3CR1 knockout per se has no effect on lesion size and early functional outcome after tMCAO. We will discuss the relevance of CX3CR1 activation for immune cell activation after stroke. Moreover, we will elaborate on the consequences of CX3CR1 knockout on different results obtained in previous studies in regard to functional outcome and lesion size after experimental stroke.
Relevance of CX3CR1 for immune cell activation after stroke
It has been shown that activation of the CX3CR1 receptor is involved in microglia/leukocyte trafficking [10, 16, 19, 25, 26] and phagocytosis [23, 27]. These processes are associated with changes in cell morphology. In the present study Sholl analysis has been performed to test if changes in ramifications in GFP+ cells depend on a functional CX3CR1 receptor in the peri-infarct area after tMCAO. We observed a significant higher number of ramifications in CX3CR1 deficient mice compared to heterozygous littermates at 2, 7, and 14 days after tMCAO. Similar results have been obtained 24 h after tMCAO . In addition, we performed Sholl analysis based on Iba-1 immunoreactivity. Also here, a significant higher number of intersections was observed in CX3CR1 deficient mice. These results might be indicative for a function of the CX3CR1 in regard to morphological changes of brain resident microglia in the postischemic hemisphere. Throughout the observation period of 14 days after tMCAO, a continuous reduction of intersections and a reduction in length of microglial branches were found in mice with a functional CX3CR1 allele. In contrast, an increase of intersections (GFP positive cells) or higher number of intersections at all time points (Iba-1 positive cells) were observed in CX3CR1 deficient mice during the first week after tMCAO with the biggest difference between the genotypes during the first week after tMCAO. Described morphological changes between the genotypes might be linked to changes of microglia function, such as phagocytosis, as shown previously [20, 23, 27]. Our results may also support earlier studies describing reduced phagocytosis in cultivated wildtype microglia cell treated with fractalkine . Differences in microglia morphology between the genotypes might also be explained by their susceptibility to the endogenous CX3CR1 ligand fractalkine. We have shown that AMD3100, an irreversible antagonist on the CXCR4 and partial agonist on the CXCR7, reduced the level of fractalkine in CX3CR1 heterozygous mice. Treatment is associated with a reduced number of microglia and a deduction of their soma size in the proximal peri-infarct area . Together, results suggest that the dynamics of microglia morphology and activation are a very fine tuned processes. Already slightly elevated levels of fractalkine found in wildtype and heterozygous mice but also the lack of CX3CR1 and downstream signaling cascades may have consequences for microglia function such as the release of pro-inflammatory molecules .
The role of the fractalkine/CX3CR1 pathway for functional outcome and tissue damage after stroke
To reiterate, initial studies have demonstrated that the fractalkine/CX3CR1 pathway is instrumental in chemotaxis of microglia [12, 25], leukocyte trafficking and microglia/macrophage recruitment . In in vitro models fractalkine showed neuroprotective properties  by activation of neuronal cell survival pathways , and fractalkine also led to increased survival of microglia . In addition, CX3CR1 deficient mice subjected to transient occlusion of the middle cerebral artery (tMCAO) showed smaller infarct volumes compared to heterozygous and wildtype littermates analyzed 72 h after tMCAO. CX3CR1 deficient animals also showed reduced levels of pro-inflammatory cytokines. Reduced levels of pro-inflammatory cytokines and reduced numbers of accumulating immune cells, however, might have been the result of smaller infarcts [31, 32]. Findings have been confirmed in a model of permanent MCAO (pMCAO) . Moreover, administration of fractalkine to wildtype mice also resulted in smaller infarcts suggesting neuroprotective actions of the fractalkine/CX3CR1 pathway. In contrast, administration of fractalkine to fractalkine deficient mice resulted in bigger lesions. Summarizing both studies, administration of fractalkine but also CX3CR1 deficiency seem to be beneficial after experimental stroke and models of neurodegeneration . We, therefore, aimed at studying the effect of CX3CR1 deficiency on acute functional deficits and definite lesion size. As described above, no differences have been observed between CX3CR1 deficient mice and wildtype littermates. Results are based on a sufficient number of animals included in the studies, physiological parameters have been controlled tightly and mortality was not different between the study groups. Infarct size measurements have been performed based on NeuN immunoreactivity, and respective areas (see “Methods” section) have been encircled with the aid of microscopic control. In our approach we applied a commonly used mouse anti NeuN antibody, hence it needs to be considered that subsequent application of secondary anti mouse antibodies resulted in crossreactivity with extravasated IgG’s in the ischemic core. This can be eliminated by the use of mouse anti NeuN antibodies directly conjugated with biotin or anti NeuN antibodies from other species. However, detection of IgG extravasation can facilitate distinction between intact and ischemic brain tissue. Shrinkage of tissue has been eliminated by indirect infarct size measurements as described previously . The approach to stain postischemic brains (TTC versus NeuN immunohistochemistry) as well as different time points of infarct size measurements may explain the differences between the studies. Although different methods are available to evaluate the size of brain lesions after experimental stroke  our data suggest that results from low resolution methods such as TTC or MRI should be verified by immunohistochemistry. In addition, analysis of ischemic lesions should be performed at a time point when the infarct has been subsided and edema has been resolved. Since differences are apparent between experimental stroke models and investigators, analysis should not be performed before day 7 after stroke. From our studies we conclude that CX3CR1 deficiency has no impact on acute functional outcome and did not affect lesion size after tMCAO.
We show that CX3CR1 significantly contributes to changes in microglia morphology in the proximal peri-infarct area following tMCAO. Morphological alterations suggest a shift in microglia functionality integrated in the inflammatory response after stroke. We also demonstrate that CX3CR1 deficiency has no beneficial effect on lesion size neither it affects early functional outcome after stroke. This, in first line, can be explained by similar lesion sizes in mice of the different genetic backgrounds. Changes in the number of branches might have consequences in the interaction of microglia with other glial cell populations and neurons. Therefore, modulation of microglia in morphology and function, e.g. by pharmacological means, might be a therapeutic strategy to optimize tissue reorganization in the post-ischemic brain.
GvdM performed the immunohistochemistry experiments and the Sholl analysis and wrote the first draft of the manuscript. VH performed the surgeries. TW contributed to data analysis. KR conceived the study, contributed to data analysis and edited the manuscript. All authors read and approved the final manuscript.
We thank Kerstin Beirup and Carin Sjölund for excellent technical support.
The authors declare that they have no competing interests.
Availability of data and materials
The datasets generated during the current study are available from the corresponding author on reasonable request.
Consent for publication
All authors gave their consent to publish the manuscript.
Ethics approval and consent to participate
All animal procedures included in this study were approved by the Malmö/Lund ethical committee (Approval Numbers M300-09 and M37-11).
This study was supported by the Swedish Research Council (Grant 2011-2684 and Grant 2011-2652), the EU 7th work program through the European Stroke Network (Grant No. 201024), the Hans-Christian and Alice Wachtmeister Foundation, the Swedish Brain Fund (Hjärnfonden), the Thorsten och Elsa Segerfalk stiftelse and the Crafoord Foundation.
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