Fig. 3
From: spargel, the PGC-1α homologue, in models of Parkinson disease in Drosophila melanogaster
Tissue specific altered srl expression in dopaminergic neurons can lead to a model of Parkinson disease Drosophila melanogaster. a Expression of srl-RNAi (UAS-srl HMS00858) driven by Ddc-Gal4 results in an increase in lifespan compared to UAS-lacZ controls. Dopaminergic srl-EY (UAS-srl EY05931) expression resulted in a decrease in lifespan compared to UAS-lacZ controls. Longevity is shown as a percent survival (P < 0.05 as determined by the Mantel-Cox Log Rank test) N ≥ 200. b Expression of srl-RNAi (UAS-srl HMS00858) and srl-EY (UAS-srl EY05931) driven by Ddc-Gal4 cause a decrease in climbing ability over time. Dopaminergic specific expression of srl-EY causes a more severe decrease, however, srl-RNAi expressing flies live longer and climb slightly worse than UAS-lacZ controls indicating a potential decrease in climbing activity compared to lifespan. Climbing ability was determined via nonlinear curve fit (CI 95 %). Error bars indicate standard error of the mean, n = 50. c Expression of srl-RNAi (UAS-srl HMS00858) driven by Ddc-Gal4 HL4.3D at 29 °C results in an increase in lifespan while srl-EY (UAS- srl EY05931) expression at 29 °C resulted in a decrease in lifespan compared to UAS-lacZ controls. Longevity is shown as a percent survival (P < 0.0001 as determined by the Mantel-Cox Log Rank test) N ≥ 150