- Poster presentation
- Open Access
Electric field modulation of theta and gamma rhythms: probe into network connectivity
BMC Neurosciencevolume 9, Article number: P8 (2008)
Introduction and background
Subthreshold electric fields are effective in suppressing epilepsy , modulating the state of single neurons via polarization , and affecting neuronal synchronization . Here we employ detailed computational modeling to test the use of global and local electric fields as a tool for dissecting neural population dynamics and connectivity. For example, modulation of cellular firing by theta rhythms plays an important role in hippocampal cognitive functions. It has been proposed that theta modulation can synchronize cell assemblies across the hippocampus; that place cell firing phase can code for the animal position; and that different phases of theta correlate with learning vs. recall. To demonstrate control of theta and gamma rhythms by electric field (EF), we developed a simplified 2-dimensional CA1 network based on  showing theta-modulated high frequency activity of pyramidal neurons . Application of EF alone was able to shift the firing mode of the simplified network in the theta-gamma range.
Methods and results
Current implementation of this model (NEURON) includes morphologically realistic pyramidal cells (P) with detailed passive, active, and synaptic properties  as well as simplified 3-compartment basket (B) and oriens (O) cells. O and B simplification reduces simulation time but still captures key determinants of the single cell response to EF (cell orientation with respect to the direction of the EF and the asymmetry of its dendritic tree). EF effects at the level of single cells in the model are consistent with previous experimental observations [2, 7]. Results obtained in our previous model  are reproduced.
We analyze two alternative network configurations: OPb, strong O-P connections and weak O-B connections; and OBp, strong O-B but weak O-P connections. OPb model predicts a critical role of oriens-bistratified cells in theta-oscillations. In the OBp configuration, two different populations of B cells appear necessary to maintain a realistic firing rate of P cells. Both basket and oriens cell populations are diverse in their morphological and electrophysiological properties and specific cellular subtypes participating in the genesis of theta and gamma rhythms are unknown. By varying composition of "unknown" populations from cells with mostly horizontal dendrites (not affected by electric field) to cells with mostly vertical asymmetric dendrites (most strongly affected by EF) we analyze EF effect on rhythm generation.
While the exact architecture underlying CA3-CA1 rhythmic activity is unknown, it might be possible to distinguish between alternative mechanisms by subthreshold electric field application.
Gluckman BJ, Nguyen H, Weinstein SL, Schiff SJ: Adaptive electric field control of epileptic seizures. J Neurosci. 2001, 21 (2): 590-600.
Bikson M, Inoue M, Akiyama H, Deans JK, Fox JE, Miyakawa H, Jefferys JGR: Effects of uniform extracellular DC electric fields on excitability in rat hippocampal slices in vitro. J Physiol. 2004, 557 (Pt 1): 175-190. 10.1113/jphysiol.2003.055772.
Park E-H, Barreto E, Gluckman BJ, Schiff SJ, So P: A model of the effects of applied electric fields on neuronal synchronization. J Comput Neurosci. 2005, 19 (1): 53-70. 10.1007/s10827-005-0214-5.
Gloveli T, Dugladze T, Rotstein HG, Traub RD, Monyer H, Heinemann U, Whittington MA, Kopell NJ: Orthogonal arrangement of rhythm-generating microcircuits in hippocampus. PNAS. 2005, 102 (37): 13295-13300. 10.1073/pnas.0506259102.
Berzhanskaya J, Gorchetchnikov A, Schiff SJ: Switching between gamma and theta: Dynamic network control using subthreshold electric fields. Neurocomputing. 2007, 70 (10–12): 2091-2095. 10.1016/j.neucom.2006.10.124.
Li , Ascoli : Computational simulation of the input-output relationship in hippocampal pyramidal cells. J Comput Neurosci. 2006, 21: 191-209. 10.1007/s10827-006-8797-z.
Berzhanskaya JB: Mechanisms of electric field suppression of neuronal activity in a hippocampal slice model of epilepsy. Epilepsia. 46 (Suppl 8): 329-
Acknowledgements and Grant support
C. McIntyre and M. Robertson for initial electric field implementation
NIMH R01MH50006 and K02MH01493, NINDS R01NS39600, NIA R01AG25633