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Phase and frequency synchronization analysis of NMDA-induced network oscillation

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Background

Synchronization is a key feature of simple systems of coupled oscillators. Several synchronization metrics exist including frequency synchronization, phase synchronization, and full synchronization. These general ideas used to analyze simple systems can also be applied to complicated systems, such as neural networks. Investigating different types of synchronization may give answers to how neurons with different properties, such as the ability to produce bursting, may interact to produce an oscillating network, reminiscent of seizure-like activity. We specifically look at the frequency and phase synchronization of cellular behavior with network oscillatory activity using specific techniques designed to analyze spike-driven data.

Methods and analysis

Network oscillations are induced in a frontal cortical slice in vitro through amplification of the NMDA conductance. Simultaneous recordings of intracellular and extracellular activity are performed and analyzed for their degree of synchronization (see below). Finally, neurons are isolated in tetrodotoxin to determine if intrinsic oscillatory activity exists.

Frequency synchronization is evaluated by comparing the power spectra (PS) of the integrated extracellular activity and the instantaneous firing rate of the cell. We calculate the PS of the unevenly sampled instantaneous firing rate using Lomb's algorithm by finding a and b such that we minimize the mean squared error between the signal and F, where F(a, b, f, t n ) = acos(2πft n ) + bsin(2πft n ) [1]. A neuron and a network are considered frequency synchronized if the frequencies of corresponding peaks in their PS have a constant ratio (Fig 1).

Figure 1
figure1

Frequency Synchronization.

Phase synchronization is visualized in two ways (Fig 2): a network burst triggered raster plot, and a histogram of phase differences between the integrated network signal and the low pass filtered event-driven cellular spiking response (derived via their Hilbert transforms [2]). A tightly peaked histogram would imply that spiking is highly correlated with a particular phase of the network oscillation.

Figure 2
figure2

Phase Synchronization.

Results and conclusions

The PS of 6/8 neurons exhibited frequency synchronization with the network bursts (Fig. 1); within this group of six, neurons exhibit a wide range of phase relationships with the network oscillation from low to high levels of phase synchronization (Fig. 2). No differences in phase or frequency synchronization have currently been found between neurons with different capabilities to produce intrinsic oscillation. These tools are needed to further investigate how neuronal firing contributes to network oscillation in both experimental and computational models of epilepsy.

References

  1. 1.

    Lomb NR: Least-squares frequency analysis of unequally spaced data. Astrophysics and Space Sci. 1976, 39: 447-462. 10.1007/BF00648343.

  2. 2.

    Pikovsky A, Rosenblum M, Kurths J: Synchronization – a universal concept in nonlinear sciences. 2001, Cambridge: Cambridge University Press

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Acknowledgements

This work was supported in part by the Epilepsy Foundation, the Falk Foundation and the Linn family.

Author information

Correspondence to Amber Martell.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Keywords

  • Power Spectrum
  • Phase Synchronization
  • Tetrodotoxin
  • Oscillatory Activity
  • Cortical Slice