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Modeling the GABA and ephaptic feedback mechanisms in cat outer retina
BMC Neurosciencevolume 9, Article number: P110 (2008)
In cat, the onset of dim backgrounds can enhance small-spot flicker responses of retinal horizontal cells . This is called background-induced flicker enhancement. A biophysical model involving neural subcircuits in the outer-plexiform layer (OPL) of the retina was proposed to explain the enhancement effect . The model states that hyperpolarization of horizontal cell dendritic terminals, conducted centripetally through gap junctions from rod-induced hyperpolarization of peripheral horizontal cells, increases the flow of calcium into the cone synaptic pedicle, stimulating transmitter release by the cone presynaptic apparatus, and resulting in an enhanced postsynaptic cone response. It remains controversial as to what causes the increased calcium entry in response to horizontal cell hyperpolarization, and two hypotheses which are hotly debated have received the most attention. The first hypothesis proposes that hyperpolarization of the horizontal cell reduces the release of a blocking agent (most likely gamma-aminobutyric acid (GABA)) from the horizontal cell, allowing more calcium to enter the cone synaptic terminal . The second hypothesis contends that the rise of calcium current is GABA independent and is caused by an ephaptic mechanism in which hemichannels (half of a gap junction) and a relatively high intersynaptic resistance play the key role [3, 4]. It speculates that hyperpolarization of horizontal cells leads to an increased hemichannel-mediated current flow through the intersynaptic cleft into the horizontal cell. As a result, the potential drop over the intersynaptic resistance will increase, and the nearby voltage-dependent calcium channels in the cone membrane will sense a local depolarization, giving rise to an augmented calcium influx. Although several experiments seem to support the ephaptic hypothesis [3, 5, 6], some recent experimental  and computational  analyses question its significance. However, it is still premature to dismiss the ephaptic hypothesis based on these recent results.
A partial differential equation model for the subcircuits in the retinal OPL that incorporates both the GABA and ephaptic mechanisms is formulated, in which the collection of horizontal cells is treated as a two-dimensional conductive sheet due to the presence of numerous gap junctions between horizontal cells. Simulations are conducted for different test regions and compared to experimental data for the enhancement effect in cat retinal horizontal cells. The results provide insight into which of the competing hypotheses for calcium entry is appropriate, and may suggest the possibility of a hybrid mechanism combining both the GABA and ephaptic hypotheses.
Pflug R, Nelson R, Ahnelt PK: Background-induced flicker enhancement in cat retinal horizontal cells. I. Temporal and spectral properties. J Neurophysiol. 1990, 64 (2): 313-325.
Nelson R, Pflug R, Baer SM: Background-induced flicker enhancement in cat retinal horizontal cells. II. Spatial properties. J Neurophysiol. 1990, 64 (2): 326-340.
Kamermans M, Fahrenfort I, Schultz K, Janssen-Bienhold U, Sjoerdsma T, Weiler R: Hemichannel-mediated inhibition in the outer retina. Science. 2001, 292 (5519): 1178-1180. 10.1126/science.1060101.
Kamermans M, Fahrenfort I: Ephaptic interactions within a chemical synapse: hemichannel-mediated ephaptic inhibition in the retina. Curr Opin Neurobiol. 2004, 14 (5): 531-541. 10.1016/j.conb.2004.08.016.
Verweij J, Kamermans M, Spekreijse H: Horizontal cells feed back to cones by shifting the cone calcium-current activation range. Vision Res. 1996, 36 (24): 3943-3953. 10.1016/S0042-6989(96)00142-3.
Fahrenfort I, Sjoerdsma T, Ripps H, Kamermans M: Cobalt ions inhibit negative feedback in the outer retina by blocking hemichannels on horizontal cells. Vis Neurosci. 2004, 21 (4): 501-511. 10.1017/S095252380421402X.
Spray DC, Ye ZC, Ransom BR: Functional connexin "hemichannels": a critical appraisal. Glia. 2006, 54 (7): 758-773. 10.1002/glia.20429.
Dmitriev AV, Mangel SC: Electrical feedback in the cone pedicle: a computational analysis. J Neurophysiol. 2006, 95 (3): 1419-1427. 10.1152/jn.00098.2005.
This work is supported by National Science Foundation Grant DMS 0718308.