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Figure 12 | BMC Neuroscience

Figure 12

From: Glutamate-induced apoptosis in neuronal cells is mediated via caspase-dependent and independent mechanisms involving calpain and caspase-3 proteases as well as apoptosis inducing factor (AIF) and this process is inhibited by equine estrogens

Figure 12

Effect of glutamate on LDH release and AIF protein levels in subcellular fractions in HT22 cells and primary cortical cells. A: primary cortical cells were isolated as described under Figure 11 and were treated with 1 mM glutamate at the indicated times. B: HT22 cells were treated with 7–8 mM glutamate at the indicated times. A, B: (I): cell death were measured by LDH release assay. Data are the mean ± SEM values from at least three experiments *P < 0.05 compared to control; A, B: (II): subcellular proteins were isolated from cytosol, nuclei, mitochondria and whole cells as described under "Methods", and then AIF protein levels were detected by Western blot analysis. Actin was used as a loading control.

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BMC Neuroscience

ISSN: 1471-2202

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