The progressive nature of Wallerian degeneration in wild-type and slow Wallerian degeneration (WldS) nerves
© Beirowski et al; licensee BioMed Central Ltd. 2005
Received: 29 September 2004
Accepted: 01 February 2005
Published: 01 February 2005
The progressive nature of Wallerian degeneration has long been controversial. Conflicting reports that distal stumps of injured axons degenerate anterogradely, retrogradely, or simultaneously are based on statistical observations at discontinuous locations within the nerve, without observing any single axon at two distant points. As axon degeneration is asynchronous, there are clear advantages to longitudinal studies of individual degenerating axons. We recently validated the study of Wallerian degeneration using yellow fluorescent protein (YFP) in a small, representative population of axons, which greatly improves longitudinal imaging. Here, we apply this method to study the progressive nature of Wallerian degeneration in both wild-type and slow Wallerian degeneration (WldS) mutant mice.
In wild-type nerves, we directly observed partially fragmented axons (average 5.3%) among a majority of fully intact or degenerated axons 37–42 h after transection and 40–44 h after crush injury. Axons exist in this state only transiently, probably for less than one hour. Surprisingly, axons degenerated anterogradely after transection but retrogradely after a crush, but in both cases a sharp boundary separated intact and fragmented regions of individual axons, indicating that Wallerian degeneration progresses as a wave sequentially affecting adjacent regions of the axon. In contrast, most or all WldS axons were partially fragmented 15–25 days after nerve lesion, WldS axons degenerated anterogradely independent of lesion type, and signs of degeneration increased gradually along the nerve instead of abruptly. Furthermore, the first signs of degeneration were short constrictions, not complete breaks.
We conclude that Wallerian degeneration progresses rapidly along individual wild-type axons after a heterogeneous latent phase. The speed of progression and its ability to travel in either direction challenges earlier models in which clearance of trophic or regulatory factors by axonal transport triggers degeneration. WldS axons, once they finally degenerate, do so by a fundamentally different mechanism, indicated by differences in the rate, direction and abruptness of progression, and by different early morphological signs of degeneration. These observations suggest that WldS axons undergo a slow anterograde decay as axonal components are gradually depleted, and do not simply follow the degeneration pathway of wild-type axons at a slower rate.
Wallerian degeneration, the characteristic degeneration sequence of nerve fibres separated from their cell bodies, was described by Waller in 1850 [1, 2]. Following various forms of axon injury this rapid degeneration process begins with degradation of axoplasm and axolemma accompanied by development of axonal and myelin debris that is subsequently removed by Schwann cells and invading macrophages. In recent years it became apparent that Wallerian degeneration is initiated by an active process intrinsic to the axon that shares some principles with apoptosis [3–7]. These discoveries were firmly established by studies on the slow Wallerian degeneration (WldS) mutant mouse, in which this active process seems to be turned off. Accordingly, this mutant shows a tenfold delay in Wallerian degeneration and synapse breakdown after experimental nerve injury [8, 9]. The delay of Wallerian degeneration is an intrinsic property of the axon suggesting that glial cell and macrophage changes are secondary events . The underlying trait is carried by the autosomal dominant mutation Wld S that arose by spontaneous mutation [6, 10]. Genetic analysis has shown that the Wld S mutation on mouse chromosome 4 comprises a stable 85-kb tandem triplication [11, 12] encoding the N-terminal 70 amino acids of the multiubiquitination factor Ube4b fused in frame to the nuclear NAD producing enzyme nicotinamide mononucleotide adenylyltransferase 1 (Nmnat 1). Correspondingly, WldS mice express a novel chimeric protein (WldS protein) in neuronal nuclei that has full Nmnat 1 activity but seemingly no Ube4b function since the expressed N-terminus lacks ability for multi-ubiquitination within the ubiquitin-proteasome system (UPS) [13, 14], the molecular machinery responsible for a major pathway of cellular protein catabolism. Either only one or both parts of the nuclear WldS protein could be responsible for the phenotype through a nuclear process that has an indirect effect on the axon although recent results foster that the WldS mechanism is likely to involve a gain of function of NAD synthesis . However, inhibition of a specific step of the ubiquitin proteasome system or another modifying role of the N-terminal domain of the WldS protein remains a possibility [16, 17].
From a clinical point of view not only traumatic disorders such as nerve, spinal cord or head injury result in Wallerian degeneration  but it is now broadly accepted that Wallerian degeneration is mechanistically related to axon loss in many neurodegenerative disorders such as amyotrophic lateral sclerosis, Charcot-Marie-Tooth disease, toxic neuropathy, multiple sclerosis, and possibly Alzheimer's Disease and Parkinson's Disease [7, 14, 19–23]. Protection from neurodegenerative disorders by WldS is currently under intense investigation. The neuroprotective mutation alleviates diverse PNS axon disorders, including dysmyelination and dying back neuropathy in P0-/- mutants , motor neuropathy in pmn mutants  and axon degeneration in Vincristine and Taxol toxicity [26–28]. More recently WldS was reported to attenuate pathology in acute CNS lesions caused by stroke , Parkinson's disease , and in gracile axonal dystrophy (gad) mice, a CNS axonal spheroid pathology . A better understanding of the biological mechanism of delayed axon degeneration in neurological diseases would help to develop therapeutic methods to target axon degeneration.
Despite research extending over more than 150 years and its frequent use as tool to detect interneuronal connections in the CNS since the time of Cajal, fundamental issues of Wallerian degeneration remain unresolved and controversial even on a purely morphological level. Among these is the spatiotemporal pattern of Wallerian degeneration along the separated nerve stump. Understanding the exact pattern of spread should provide additional insights into the mechanisms of axon death and may indicate strategies to alter Wallerian degeneration in neurological disease. Shortly after the pioneering investigations of Waller, and in the following decades, there has been much debate as to whether degeneration occurs in an anterograde direction, a retrograde direction or simultaneously along the separated nerve stump axons (reviewed historically in [32–34]).
The controversy over the directionality of Wallerian degeneration has arisen partly because appropriate methods to follow axons over considerable distances did not exist until recently but also because the course of Wallerian degeneration varies with many experimental factors. Thus, spatiotemporal evolution of Wallerian degeneration depends on the laboratory animals used [32, 34–36], on the age of the animals [32, 37], on the neuroanatomical locus of study (CNS vs. PNS) [35, 38, 39], on the type of fibre analyzed (e.g. myelinated vs. unmyelinated, thick vs. thin axons) [34, 39, 40], on the type of lesion (axotomy, crush, ligature, intoxication etc.) [34, 41, 42], on the length of the remaining distal nerve stump [32, 43–45], on the criteria used for identification of fibre degeneration (e.g. myelin breakdown, axon disintegration, decay of electrophysiological activity) [32, 33, 39], on environmental factors (e.g. temperature) [6, 32, 34, 36] and many more. For example, in more modern experimentation from the last decades, Lubinska  demonstrated with the help of the teased fibre technique on myelinated fibres of the rat phrenic nerve that axonal breakdown into myelin ovoids spreads anterogradely along axons separated from their cell bodies at velocities correlated with fibre diameter and internodal length. George and Griffin  also found anterograde spread of axonal disintegration along dorsal columns of the rat following L4L5L6 radiculotomy. Contrary to these views, Lunn and colleagues  showed by means of silver-stained wholemount preparations from the peripheral nerve stump that degeneration after crushing proceeds in a retrograde direction. They also proposed a retrograde progression after sectioning, freezing or ligaturing, although this was less clear because degeneration was more complete at the time-point sampled. Electrophysiological approaches suggested that the spread of failure of conduction in degenerating mammalian nerves runs from proximal to distal after nerve transection . In cell culture studies using dorsal root ganglions (DRG) explants membrane beading, blebbing, fragmentation and Annexin V staining progressed along interrupted neurites in an anterograde direction with a rate comparable to that of slow axonal transport . Taking secondary changes after axon disintegration into consideration, Bendszus and colleagues  tracked an anterograde spatiotemporal course of macrophage infiltration after acute peripheral nerve injury in rats. While most of the above mentioned investigators concluded a progressive nature of Wallerian degeneration from the appearance of degeneration gradients along injured nerves other authors did not observe any evidence for an anterograde or retrograde pattern of axonal degeneration [52, 53].
In view of the contradictions and anomalies in the previous literature, we have reassessed the directionality of Wallerian degeneration using a recently introduced technique to visualize individual fluorescent axons over cm-long distances during degeneration . This was made possible by using nerves from transgenic mice expressing Yellow Fluorescent Protein (YFP) in representative subsets of axons, which presents a simplified image of peripheral nerve . No method existed until recently to follow up individual axons undergoing Wallerian degeneration over a considerable length. Here we compared the progression of Wallerian degeneration along single axons traced over lengths of approximately 2.5 cm. Specifically, we have tested a key prediction of all progressive models: that it should be possible to image axons degenerated at one end but not at the other. We detected such axons and showed that Wallerian degeneration in wild-type peripheral nerve is a rapid, asynchronous, progressive and wave-like process that can change its orientation depending on the lesion type.
To our knowledge there have been no reports about the spatiotemporal pattern of the much-delayed axon degeneration in peripheral WldS nerves that could yield important clues for understanding classic Wallerian degeneration. Therefore, we also report a detailed characterisation of injury-induced axon degeneration in slow Wallerian degeneration mutant mice in order to determine whether axons degenerate with a similar spatial evolution to that in wild-type mice, but in "slow motion", or whether the process is fundamentally distinct. We report a series of differences between axon degeneration in wild-type and WldS mice, suggesting that irreversible injury in axons where Wallerian degeneration is blocked eventually leads to a different pathway of degeneration.
YFP labelled wild-type axons fragment abruptly and asynchronously after a latent phase of approximately 36–44 h
Wallerian degeneration in wild-type nerves progresses anterogradely after nerve transection and retrogradely after nerve crush
Summarising all these quantification results of cut and crush lesions at time points where partially fragmented fibres were observed, on average 94.8 % of all axons were either completely intact or fragmented and 5.2 % showed a gradient of Wallerian degeneration, whose orientation depended on the lesion type.
A wave of axonal fragmentation propagates rapidly along individual wild-type axons and the axon population degenerates asynchronously
To study further the gradients of axonal fragmentation both after cut and crush injury (Fig. 3, 4) we quantified the number of axonal breaks along partially and totally fragmented axons. Firstly, this was a way to distinguish between a locally restricted wave of fragmentation such that entirely intact lengths of axon suddenly change into entirely fragmented lengths, and a more gradual fragmentation process that would result in a few interruptions that become more frequent further along the axon. Secondly, by this approach we tried to get insight on the question of whether axons assigned into the group "entirely fragmented" continue to break into smaller fragments leading to a gradient of fragment size along the nerve.
In order to determine whether the anterograde and retrograde fragmentation wave runs at the same velocity along the axon we next estimated the rate of progression. As the average axon length measured was 24 mm and the majority of axons must have entirely fragmented between 41 h and 42 h after transection (significant difference between percentage of entirely degenerated axons at 42 h and entirely plus partially degenerated axons at 41 h in Student t-test) (Fig. 2A), the minimal velocity for the degeneration wave is 24 mm/h. Analogously, after crush lesion the calculated velocities of the retrograde degeneration wave is also at least 24 mm/h (Fig. 2B), as the majority of axons fragmented between 43 h and 44 h after the lesion (significant difference between entirely degenerated axons at 44 h and entirely plus partially degenerated axons at 43 h in Student t-test). Thus, the rates of Wallerian degeneration progression are similar or possibly even equal in these opposite directions, but with a faster initiation of the fragmentation wave after transection.
We then tested whether our crush lesions interrupted axon continuity as in nerve transection, because a failure to do so could underlie the different direction of propagation in crushed nerves (see Discussion). In fluorescent wholemount preparations of crushed nerve segments we found continuous longitudinal YFP signals across the crush site, and in teased fibre bundles of osmificated nerve segments after crush injury, a majority of fibres remained continuous across the crush site (see additional data file Add Fig 1.pdf). These data are consistent with the axonal membrane remaining intact after 30 sec nerve crush, unlike that of a transected nerve.
The observation that at early time points after cut and crush injury some axons had already fragmented or started to fragment while the majority is still intact (Fig. 1, 2) together with the variable localisation of the fragmentation wave front along different axons at one time point (Fig. 6) indicates that Wallerian degeneration is asynchronous among the population of axons in a peripheral nerve. This probably reflects both differences in the timing of onset of degeneration and varying velocities of propagation in axons of different thickness that cannot be distinguished by our imaging approaches. Furthermore, the observation that the transition between intact and degenerated regions can be 19–21 mm distal to the crush within 44 h rules out regeneration as a possible source of error.
In contrast to wild-type nerves injured WldSsciatic and tibial nerves degenerate anterogradely independent of the lesion type
Remarkably, in view of data reported in wild-type mice, a crush injury of the proximal sciatic nerve also resulted in anterograde progression in WldS sciatic/tibial nerve that was evident with a more moderate gradient after 20–30 days. Twenty days after sciatic nerve crush 70.0 % of all myelinated axons were preserved a few millimetres distal to the lesion (Fig. 8F, G, H) while 94.8 % of these fibres were preserved at the distal tibial nerve more than 20 millimetres away from the point of lesion (Fig. 8F, I, J). Similarly, 25 and 30 days after crush lesion we observed an increase in preserved axon numbers from proximal to distal along the sciatic-tibial nerve distance (Fig. 8F). Even 30 days after transection or crush lesion there were many protected distal axons in tibial nerve (Fig. 8A, F). By contrast, the degeneration of all distal fibres in wild-type mice was complete within two days (see above). Thus, while axon degeneration in more proximal regions is delayed approximately tenfold by WldS after a lesion, the delay in more distal regions is at least twenty fold. In summary, quantification of axon preservation assessed by light and electron microscopy is sufficient to indicate a marked anterograde direction of axon degeneration both after transection and crush injury of peripheral WldS nerves.
Anterograde degeneration of individual YFP labelled WldSaxons is slowly progressive
We then followed up the earlier EM experiments using crushed nerves from WldS/YFP-H double mutant mice to study the directionality of axon degeneration in WldS nerves using this very different method. Once again, the spatio-temporal pattern of axon degeneration after crush injury in WldS mice was very similar to that after transection injury, contrasting with wild-type mice where directionality depends on lesion type. Correspondingly, 15 days after crush lesion we counted 84.60 % of all YFP labelled WldS axons with an anterograde gradient of degenerative changes (Fig. 9). An example of such an individual axon is shown in the additional data file Add Fig 2.pdf. However, crushed WldS axons more frequently showed end bulbs at the proximal end of the distal stump, which were often very large, (red arrow in overview of Add Fig 2.pdf) and subsequent multiple axonal swellings (inset 1 in Add Fig 2.pdf). This feature was far more prominent in crushed WldS axons than in the transection experiment where we observed end bulbs just occasionally. Further distally these swellings disappear with remaining axonal constrictions and breaks (inset 2 in Add Fig 2.pdf) representing incomplete fragmentation. As in transected nerves, distal parts of the crushed WldS axon were free of degeneration signs (inset 3 and 4 in Add Fig 2.pdf). Compared to transection lesion, more axons at 15 days remained entirely intact (15.4 %) (Fig. 9). By 20 days after nerve crush of WldS axons, proximal fragmentation became more prominent with fully separated fragments (inset 1 in additional data file Add Fig 3.pdf) while more distal regions of the same axons were again incompletely fragmented (inset 2 in Add Fig 3.pdf) and further distal still lacked any degeneration signs (insets 3 + 4 in Add Fig 3.pdf). The proximal end bulbs and localised swellings were larger than at 15 days (red arrow in overview of Add Fig 3.pdf), possibly due to continued accumulation of retrogradely transported material.
In summary, quantification 20 days after crush lesion revealed that all axons showed anterograde gradients of complete or incomplete fragmentation (Fig. 9). Thus, the YFP-H studies confirmed our light and electron microscopy observations that delayed degeneration in individual WldS axons is directional with an exclusively anterograde pattern both after transection and crush injury. This pattern of degeneration is qualitatively different from that in wild-type mice, which shows asynchronous, bidirectional fragmentation and degeneration.
WldSaxons show a continuous gradient of axon degeneration that moves with a velocity similar to that of slow axonal transport
We have shown that the fragmentation of axons undergoing Wallerian degeneration in a mixed wild-type peripheral nerve is a rapid, asynchronous and progressive process. By using a recently developed method to visualise individual axons over cm-long distances, and by targeting a short critical period during which nearly all axons degenerate, we have made the first observations in vivo of partially fragmented individual axons and thus determined the directionality and the wave-like nature of Wallerian degeneration, as well as estimating its velocity. Furthermore, we have shown that nerves of WldS mutant mice undergo a fundamentally distinct process rather than simply following the same pathway in slow motion.
Lesioned wild-type axons remain morphologically normal for a latency period of ca. 36–44 hours, which depends on lesion type and individual axonal properties [38, 39, 44, 46, 60]. Each axon then undergoes a catastrophic process in which at least 24 mm of the distal stump fragments entirely within an hour. The propagation rate of at least 24 mm/h is considerably faster than reported in rat dorsal column (3 mm/h) , rat phrenic nerve (up to 10.4 mm/h)  and in primary culture (ca. 0.4 mm/h) , probably reflecting differences in neuronal subtype and context. For example, slower propagation of Wallerian degeneration in the CNS is suggested by the observation of an anterograde spread in the gracile tract following a dorsal root lesion, whereas the spread of degeneration within the root itself was too short-lived to be resolved by the methods used . Wallerian degeneration may also propagate more slowly in longer axons, which could account for differences between mice and rats [44, 45], and there may be many reasons why the propagation rate in vitro could differ from that in vivo. Nevertheless, while the propagation rate may differ, the anterograde degeneration after axon transection is a consistent feature of each of these studies.
We have shown that Wallerian degeneration in wild-type nerves is a wave-like process that can travel in either direction along the axon, depending on lesion type. 29 partially fragmented axons were observed, and all showed a sharp boundary between fragmented and non-fragmented zones, such that all axon regions up to the wavefront were degenerated and all regions beyond it remained intact. Fragmentation had reached different points along the nerve in different individual axons (Fig. 6), reflecting asynchronicity of the onset and rate of degeneration. The wave-like propagation of Wallerian degeneration has been proposed before [44, 46, 47, 49, 61], and especially Lubinska [44, 46] has shown that Wallerian degeneration of the distal stump progresses centrifugally by jumping from one internode to another, but this is the first time the wavefront has actually been observed. Of the 29 partially fragmented axons, 17 transected axons were fragmented only at their proximal ends and 12 crushed axons were fragmented only at their distal ends (Fig. 2, 3, 4). Differences between cut and crushed nerves have been suggested before [48, 53] and axons cut at both ends also exhibit a retrograde degeneration component , but this is the first demonstration that two different lesions at the same site in the same nerve cause different directions of degeneration. The mechanistic basis of this surprising observation remains unknown, but some models are outlined below.
Thus, we consider also an alternative model in which short regions of the axon membrane become permeabilised to calcium ions and this feature moves rapidly as a wave along the axon. Fibre degeneration requires accumulation of axoplasmic calcium [65, 66], which probably activates the cystein protease calpain [67–70]. Once inside the axon, calcium ions could diffuse to the immediately neighbouring axoplasm and activate calpain, leading to degradation of axoplasmic and membrane proteins, and thus permeabilisation of the next segment. Such a membrane associated Ca2+ influx hypothesis was proposed by Schlaepfer for the first time [71, 72] and developed further involving calcium channels in more modern studies [73, 74]. LoPachin and Lehning  reported calcium entry linked to membrane depolarisation through reverse Na2+-Ca2+-exchange, leading to a steady rise in intra-axonal calcium and calcium accumulation has been observed beneath Schmidt Lantermann clefts at distal sites 4 h after injury . Once the threshold for calpain activation is exceeded, a wave of degeneration could be initiated and then propagate rapidly in either direction as outlined above.
There are several possible reasons why degeneration may begin proximally in a transected nerve but distally in a crushed nerve. The proximal end of a transected distal stump is especially vulnerable because of the exposure of the axoplasm to the external ionic environment, and because all extra-axonal structures that normally support the axon have been totally disrupted, e.g., blood vessels, Schwann cells, extracellular matrix, perineurium. One of these factors may cause a calcium entry wave to begin at this point. In contrast, intact endoneural blood vessels can be found close to a nerve crush [77, 78], the epi- and perineurium tubes are maintained at the site of crush [78–81], and some nerve crush protocols do not break axon continuity [82–85], so that Wallerian or Wallerian-like degeneration occurred only many days after compression or not at all. More specifically, even application of longer high pressure injuries with a minimum of shear forces may squeeze out axoplasm into adjacent parts of the axon rather than interrupting the axolemma preserving nerve conduction monitored electrophysiologically . In our nerves we observed continuous longitudinal YFP signals across crush sites immediately after lesioning, indicating that at least some axons were not transected by the direct effect of crushing. We also observed many preserved fibres crossing the crush site when we fixed and then partially teased crushed nerves to generate small bundles where individual fibres were easily identifiable (see additional data file Add Fig 1. pdf). Thus, the proximal end of the distal stump may be less vulnerable than after transection, and fragmentation may begin instead at the distal end because this is the hardest part to supply with everything the axon needs to survive.
We observed a series of differences in the pattern of Wallerian degeneration in WldS nerves that are incompatible with delayed axon degeneration following a similar mechanism to Wallerian degeneration in wild-type nerves, only slower. The spread of degeneration along WldS nerves is around 100-fold slower, axon degeneration is more synchronous, at least relative to how long it takes to occur, it progresses in a proximal to distal direction in crushed nerves as well as transected nerves, there is a continuous gradient of degeneration along the length of the axon rather than an abrupt change at a boundary, and the first sign of axon degeneration is a constriction rather than a complete interruption. We therefore propose that the ultimate degeneration of axons in WldS mice be termed "slow anterograde axon decay" rather than Wallerian degeneration as summarised schematically in Fig. 13.
Based on these differences, we propose that injured WldS axons eventually undergo a passive process of atrophy, rather than an active process of self-destruction similar to apoptosis that appears to take place in wild-type axons [5, 7, 15, 16, 86]. It is likely that preserved axonal proteins will eventually be degraded by catabolic processes and may not be replaced by significant new synthesis, even if Wallerian degeneration is completely prevented. A direct indication of this is our observation in primary neuronal cultures of significant atrophy of distal neurites when their degeneration is delayed by WldS (data not shown). Even the fact that some proteins are synthesised locally in axons [57, 87–89] may not be sufficient to prevent the eventual depletion of protein in severed WldS axons, as it remains unclear which proteins are made there and in what quantities.
We discuss two models to explain the slow anterograde progression of degeneration along WldS axons: one based on slow axonal transport and the other on a temperature gradient along the limb. The gradual nature of axonal atrophy in WldS makes it difficult to be precise about the rate at which it progresses along the axon, but it is certainly not incompatible with the velocity of slow axonal transport of 0.1–3.0 mm/day [56–58, 90]. Clearance of structural proteins by slow anterograde transport, added to their gradual depletion by protein turnover, could cause the protein content at the proximal end of the distal stump to drop below the threshold level needed to maintain axon integrity. Bidirectional transport of cytoskeletal components continues in transected WldS nerves, leading to localised neurofilament-depleted constrictions and terminal and intermediate swellings containing disorganised neurofilaments [91, 92]. We have made similar observations in YFP labelled WldS axons, and additionally report a gradient of such features along the nerve. A net anterograde movement of cytoskeletal proteins could therefore underlie the anterograde gradient of axonal atrophy in WldS axons injured for many days.
Alternatively, a proximal-distal decreasing gradient of temperature along the limb could underlie the observed difference in degeneration rate at different points in the WldS nerve. A decrease in temperature has been shown to delay degeneration both in wild-type and WldS axons after injury [52, 74, 93–96]. In wild-type nerves, a temperature gradient explains neither the different directions of propagation after transection and crush injury, nor the sharp boundary between intact and degenerated regions. However, in WldS axons there is a proximal to distal gradient of degeneration regardless of lesion type and the change from intact to degenerated is a gradual one. Thus, a temperature gradient could play a more important role here.
The extremely long survival of distal tibial nerve following injury in WldS is in marked contrast to the presynaptic nerve terminal at the neuromuscular junction, which is the first structure to degenerate in both wild-type and WldS nerves [2, 8, 46]. Intramuscular nerve also degenerates early, at least in WldS heterozygotes (L. Fan and R.R. Ribchester, unpublished). This supports the hypothesis of compartmentalised degeneration mechanisms of axons and synapses  and suggests that a clear boundary exists between the two domains. The location and nature of this boundary could hold important clues for determining the mechanism of both Wallerian degeneration and synapse degeneration.
Finally, the methods we report here could now be applied to study spontaneous nerve degeneration in 'dying-back' disease. The 'dying-back' model also predicts the transitory existence of partially degenerated axons, but as in Wallerian degeneration such axons have never been directly observed and there is no indication of the speed of 'dying-back' of individual axons [98, 99]. There are interesting parallels between axon degeneration after nerve crush and 'dying-back', as both can be delayed by the Wld S gene and the direction of degeneration is also shared . Thus, it is an intriguing possibility that the speed of propagation is equally rapid and asynchronous. If it is a similarly catastrophic event, what can stop it progressing back to the cell body leading to neuron death? In some cases neuronal death does appear to be the outcome [25, 100], whereas in others, proximal axons and their cell bodies somehow survive  and it is important to find out why. Future prospects include direct observation of the progression of a degeneration boundary along YFP labelled axons in vivo after nerve lesion or in disease, once methods for in vivo imaging of single axons become more refined and more readily available, e.g., Cell ViZio, Mauna Kea Technologies .
In summary, we report the first direct observation of partially degenerated single axons in lesioned nerves undergoing Wallerian degeneration, indicating that Wallerian degeneration propagates in wild-type nerves as a wave whose speed is at least as fast as the highest reported rate of fast axonal transport. It could be faster still in mouse sciatic and tibial nerve. The direction of degeneration is proximal to distal after a cut, but the reverse after a crush. For now the mechanism remains unknown, but these observations will ultimately need to be explained in any comprehensive model of the Wallerian degeneration mechanism. Injury-induced axon degeneration in WldS nerves is also progressive, but differences in the topographic pattern and morphology of degeneration indicate a fundamentally different process from that in wild-type nerves. We propose that WldS axons ultimately undergo atrophy, in a passive process similar to that which Wallerian degeneration was once thought to be.
Crossbreeding and genotyping of transgenic mice
Crossbreeding and genotyping of the YFP-H line  and triple heterozygote mice carrying the original Wld S mutation, the transgenic Wld S mutation (tg-Wld S )  and the YFP-H gene was performed as previously described . Triple heterozygote mice rather than WldS/YFP-H mice were used purely for reasons of convenience in their breeding. They express similar levels of WldS protein as homozygous natural mutant WldS mice and display a similar retarded time-course of axon degeneration. They additionally express YFP in approximately 3% of myelinated motor and sensory fibres in the PNS, equal to the original YFP-H line obtained from the Jackson Laboratories.
Sciatic nerve lesions
Six- to 10-week-old mice from the YFP-H line and triple heterozygote mice for the second part of the study were anaesthetised by intraperitoneal injection of Ketanest (5 mg/kg; Parke Davis) and Rompun (100 mg/kg; Bayer), sciatic nerves were transected or crushed firmly close to the Foramen intrapiriforme and the wound was closed with a single suture. Complete nerve transection was performed with conventional surgical scissors and crush lesion was achieved with fine watchmaker's forceps (model: micrscopic forceps bent No. 7, Aesculap BD 333R, Germany) for 30 seconds. The continuity of the sciatic nerve was always preserved after crush lesion as checked in situ. For light and electron microscopy we removed distal ~2.5 centimeter long nerve stumps after 15 (transection only), 20, 25 and 30 days in triple heterozygote mice following intracardial perfusion. The first 2 millimeters of the distal nerve stump were discarded to reduce artifacts, and the next 2 mm from the most proximal and distal end of the peripheral nerve stump was prepared for Durcupan embedding and electron microscopy.
For conventional fluorescence microscopy and confocal tracing of individual YFP labelled wild-type axons after 34 h, 37 h, 40 h, 41 h, 42 h and 48 h following cut lesion and 37 h, 40 h, 42 h, 43 h, 44 h and 48 h following crush lesion the operated YFP-H mice were sacrificed by cervical dislocation and nerve segments prepared as follows.
For confocal tracing of individual YFP labelled WldS axons from triple heterozygote mice we dissected sciatic-tibial nerve segments after 12.5 d (transection only), 15d, 17.5 d (transection only), 20 d and 22.5 d (transection only). For each investigated time-point 2 – 3 YFP-H or triple heterozygote mice were operated.
Assessment of axonal continuity in crushed YFP-H nerve segments
Sciatic nerves of mice from the YFP-H line were crushed as described in the paragraph above and the short nerve segment containing the crush site immediately removed after lesion, freed from surrounding connective tissue and subsequently either prepared for wholemount fluorescence embedding as described previously  or for tissue osmification. For the latter the crushed segment was immersion-fixed for four hours in 10% PFA in 0.1 M PBS, washed three times in 0.1 M PBS for 10 minutes and osmificated in aqueous osmium-tetroxide (1 %) solution for 90 minutes. After rinsing in fresh 0.1 M PBS individual axon bundles were teased from the crushed segment using fine syringe needles (Neoject 26 G × 1/2, Dispomed WITT, Germany) and mounted on conventional glass slides. Fluorescence imaging of crushed YFP-H nerve segments and light microscopy of osmificated fibre bundles was carried out using an Olympus IX 81 inverted microscope coupled to a Olympus U-TV0.5XC digital camera system.
Intracardial perfusion for light and electron microscopy of semithin and ultrathin preparations
After sternotomy under deep anaesthesia mice were killed by cardiac puncture and instantly intracardially perfused first with a solution containing 10 000 i.e./l heparin (Liquemin N 25 000, Hoffmann-La Roche) and 1 % procainhydrochloride in 0.1 M PBS for 30 s and then with fresh half-strength Karnovsky's fixative (4 % paraformaldehyde, 2 % glutardialdehyde in 0.1 M sodium cacodylate, pH 7.3).
Light and electron microscopy
Nerve samples for light and electron microscopy were embedded in Durcupan and further processed for examination with a Zeiss Axiophot light microscope and Zeiss EM 902 electron microscope as described previously .
Morphological quantification of axon preservation in light and electron micrographs
The percentage of preserved myelinated axons in proximal and distal ends of peripheral nerve stumps from triple heterozygote mice after transection and crush injury was determined as described previously [13, 54].
Conventional fluorescence microscopy and confocal tracing of individual YFP labelled axons after various lesion times
In both YFP-H and triple heterozygote mice after cut or crush lesion the entire nerve distances from the proximal sciatic to the distal tibial nerve were carefully excised, the perineurium and the branch of the commune fibular nerve removed and the remaining ~2.5 centimeter long stumps treated for wholemount fluorescence preparation using Vectashield Mounting Medium (Vector Laboratories) as described previously . For rough orientation the wholemount preparations were photomicrographed at the proximal and distal end of the excised nerve segment using a Zeiss Axiophot microscope connected to a digital camera system (Universal Imaging Corporation). High resolution confocal composite presentation of the entire intra-nerve course of individual degenerating YFP-labelled wild-type or WldS axons running through the excised stumps over their whole length was achieved as described previously . Single images were obtained with LaserSharp 2000 software connected to a Biorad Radiance 2000 laser scanning system (Hemel Hempsted, UK) and composite pictures on a black background were created using Adobe Photoshop.
Imaging of restricted transition zones dividing intact and fragmented axon regions in YFP-H nerves was performed under highest possible resolution with a Zeiss LSM 510 META confocal system (LSM Software Release 3.2) coupled to a Zeiss Axiovert 200 microscope.
Quantification of intact and degenerated YFP labelled wild-type and WldSaxons in peripheral nerve stumps after cut and crush injury
For the first part of the study focusing on sciatic/tibial nerves from the YFP-H line in each wholemount preparation (2 – 3 nerve preparations per time point) YFP labelled wild-type axons running continuously through the excised peripheral nerve stump after cut and crush injury were traced individually with the laser scanning confocal microscope under high resolution and divided into four groups: axons without any features of axonal disintegration were assigned to the group "intact". Axons that showed fragmentation of the longitudinal YFP signal over the whole length of the fibre were assigned to the group "entirely fragmented". Axons without any sign of fragmentation at one end but clear axon breakdown at the other end were assigned either into the group "fragmented with anterograde gradient" or "fragmented with retrograde gradient" depending whether the fragmentation appeared close to the lesion point or at the most distal point of the tibial nerve segment. For the second part of the study dealing with triple heterozygote mice YFP labelled WldS axons in wholemount peripheral nerve stumps (2–3 nerve preparations for each timepoint) after transection and crush injury were equally traced individually with the BioRad Radiance 2000 laser scanning confocal microscope under high resolution, and divided into two groups: axons without any features of axonal disintegration were counted and assigned to the group "intact". Fibres that showed degeneration signs like constrictions or interruptions of the longitudinal YFP signal at the proximal site were associated into the group "fragmented with anterograde gradient". Means and standard deviations for all experiments were calculated using Microsoft Excel.
Quantification of axonal degeneration signs along YFP positive axons
The length of degenerated YFP labelled wild-type and WldS axons was measured during the tracing process with the confocal laser scanning microscope and the distribution of axonal interruptions (both in wild-type and WldS axons) and/or constrictions (only in WldS axons) was graphed against the distance in mm from the cut or crush point. Means and standard deviations were calculated using Microsoft Excel.
All animal experiments were carried out under appropriate German licences: Tierschutzgenehmigung K 13, 11/00 and Anzeige K30/99.
This study was supported by the Federal Ministry of Education and Research (FKZ: 01 KS 9502), Center for Molecular Medicine (CMMC), University of Cologne (BB, RA, DW, DSG and MCP), the Koeln Fortune Program / Faculty of Medicine, University of Cologne (BB), ALS Association (USA) Grant 167 (RA) and a Wellcome Trust Biomedical Collaboration Grant (RRR and MPC).
We thank Dr. Simon Walker (The Babraham Institute) and Adrian Thomson (Division of Neuroscience, University of Edinburgh) for assistance and technical advice on confocal microscopy.
The work forms part of the doctoral thesis of Bogdan Beirowski (University of Cologne).
- Waller A: Experiments on the section of glossopharyngeal and hypoglossal nerves of the frog and observations of the alternatives produced thereby in the structure of their primitive fibers. Philos Trans R Soc Lond Biol. 1850, 140: 423.Google Scholar
- Stoll G, Jander S, Myers RR: Degeneration and regeneration of the peripheral nervous system: from Augustus Waller's observations to neuroinflammation. J Peripher Nerv Syst. 2002, 7: 13-27. 10.1046/j.1529-8027.2002.02002.x.PubMedGoogle Scholar
- Glass JD, Brushart TM, George EB, Griffin JW: Prolonged survival of transected nerve fibres in C57BL/Ola mice is an intrinsic characteristic of the axon. J Neurocytol. 1993, 22: 311-321.PubMedGoogle Scholar
- Deckwerth TL, Johnson EM: Neurites can remain viable after destruction of the neuronal soma by programmed cell death (apoptosis). Dev Biol. 1994, 165: 63-72. 10.1006/dbio.1994.1234.PubMedGoogle Scholar
- Buckmaster EA, Perry VH, Brown MC: The rate of Wallerian degeneration in cultured neurons from wild type and C57BL/WldS mice depends on time in culture and may be extended in the presence of elevated K+ levels. Eur J Neurosci. 1995, 7: 1596-1602.PubMedGoogle Scholar
- Perry VH, Lunn ER, Brown MC, Cahusac S, Gordon S: Evidence that the Rate of Wallerian Degeneration is Controlled by a Single Autosomal Dominant Gene. Eur J Neurosci. 1990, 2: 408-413.PubMedGoogle Scholar
- Raff MC, Whitmore AV, Finn JT: Axonal self-destruction and neurodegeneration. Science. 2002, 296: 868-871. 10.1126/science.1068613.PubMedGoogle Scholar
- Lunn ER, Perry VH, Brown MC, Rosen H, Gordon S: Absence of Wallerian Degeneration does not Hinder Regeneration in Peripheral Nerve. Eur J Neurosci. 1989, 1: 27-33.PubMedGoogle Scholar
- Ribchester RR, Tsao JW, Barry JA, Asgari-Jirhandeh N, Perry VH, Brown MC: Persistence of neuromuscular junctions after axotomy in mice with slow Wallerian degeneration (C57BL/WldS). Eur J Neurosci. 1995, 7: 1641-1650.PubMedGoogle Scholar
- Conforti L, Tarlton A, Mack TG, Mi W, Buckmaster EA, Wagner D, Perry VH, Coleman MP: A Ufd2/D4Cole1e chimeric protein and overexpression of Rbp7 in the slow Wallerian degeneration (WldS) mouse. Proc Natl Acad Sci U S A. 2000, 97: 11377-11382. 10.1073/pnas.97.21.11377.PubMed CentralPubMedGoogle Scholar
- Coleman MP, Conforti L, Buckmaster EA, Tarlton A, Ewing RM, Brown MC, Lyon MF, Perry VH: An 85-kb tandem triplication in the slow Wallerian degeneration (Wlds) mouse. Proc Natl Acad Sci U S A. 1998, 95: 9985-9990. 10.1073/pnas.95.17.9985.PubMed CentralPubMedGoogle Scholar
- Mi W, Glass JD, Coleman MP: Stable inheritance of an 85-kb triplication in C57BL/WldS mice. Mutat Res. 2003, 526: 33-37.PubMedGoogle Scholar
- Mack TG, Reiner M, Beirowski B, Mi W, Emanuelli M, Wagner D, Thomson D, Gillingwater T, Court F, Conforti L, Fernando FS, Tarlton A, Andressen C, Addicks K, Magni G, Ribchester RR, Perry VH, Coleman MP: Wallerian degeneration of injured axons and synapses is delayed by a Ube4b/Nmnat chimeric gene. Nat Neurosci. 2001, 4: 1199-1206. 10.1038/nn770.PubMedGoogle Scholar
- Coleman MP, Perry VH: Axon pathology in neurological disease: a neglected therapeutic target. Trends Neurosci. 2002, 25: 532-537. 10.1016/S0166-2236(02)02255-5.PubMedGoogle Scholar
- Araki T, Sasaki Y, Milbrandt J: Increased nuclear NAD biosynthesis and SIRT1 activation prevent axonal degeneration. Science. 2004, 305: 1010-1013. 10.1126/science.1098014.PubMedGoogle Scholar
- Coleman MP, Ribchester RR: Programmed axon death, synaptic dysfunction and the ubiquitin proteasome system. Curr Drug Targets CNS Neurol Disord. 2004, 3: 227-238.PubMedGoogle Scholar
- Zhai Q, Wang J, Kim A, Liu Q, Watts R, Hoopfer E, Mitchison T, Luo L, He Z: Involvement of the ubiquitin-proteasome system in the early stages of wallerian degeneration. Neuron. 2003, 39: 217-225. 10.1016/S0896-6273(03)00429-X.PubMedGoogle Scholar
- Maxwell WL, Povlishock JT, Graham DL: A mechanistic analysis of nondisruptive axonal injury: a review. J Neurotrauma. 1997, 14: 419-440.PubMedGoogle Scholar
- Bjartmar C, Yin X, Trapp BD: Axonal pathology in myelin disorders. J Neurocytol. 1999, 28: 383-395. 10.1023/A:1007010205037.PubMedGoogle Scholar
- Griffin JW, George EB, Chaudhry V: Wallerian degeneration in peripheral nerve disease. Baillieres Clin Neurol. 1996, 5: 65-75.PubMedGoogle Scholar
- Zhao C, Takita J, Tanaka Y, Setou M, Nakagawa T, Takeda S, Yang HW, Terada S, Nakata T, Takei Y, Saito M, Tsuji S, Hayashi Y, Hirokawa N: Charcot-Marie-Tooth disease type 2A caused by mutation in a microtubule motor KIF1Bbeta. Cell. 2001, 105: 587-597. 10.1016/S0092-8674(01)00363-4.PubMedGoogle Scholar
- Trapp BD, Peterson J, Ransohoff RM, Rudick R, Mork S, Bo L: Axonal transection in the lesions of multiple sclerosis. N Engl J Med. 1998, 338: 278-285. 10.1056/NEJM199801293380502.PubMedGoogle Scholar
- Wang MS, Wu Y, Culver DG, Glass JD: Pathogenesis of axonal degeneration: parallels between Wallerian degeneration and vincristine neuropathy. J Neuropathol Exp Neurol. 2000, 59: 599-606.PubMedGoogle Scholar
- Samsam M, Mi W, Wessig C, Zielasek J, Toyka KV, Coleman MP, Martini R: The wlds mutation delays robust loss of motor and sensory axons in a genetic model for myelin-related axonopathy. J Neurosci. 2003, 23: 2833-2839.PubMedGoogle Scholar
- Ferri A, Sanes JR, Coleman MP, Cunningham JM, Kato AC: Inhibiting axon degeneration and synapse loss attenuates apoptosis and disease progression in a mouse model of motoneuron disease. Curr Biol. 2003, 13: 669-673. 10.1016/S0960-9822(03)00206-9.PubMedGoogle Scholar
- Wang MS, Davis AA, Culver DG, Glass JD: WldS mice are resistant to paclitaxel (taxol) neuropathy. Ann Neurol. 2002, 52: 442-447. 10.1002/ana.10300.PubMedGoogle Scholar
- Wang MS, Fang G, Culver DG, Davis AA, Rich MM, Glass JD: The WldS protein protects against axonal degeneration: a model of gene therapy for peripheral neuropathy. Ann Neurol. 2001, 50: 773-779. 10.1002/ana.10039.PubMedGoogle Scholar
- Wang M, Wu Y, Culver DG, Glass JD: The gene for slow Wallerian degeneration (Wld(s)) is also protective against vincristine neuropathy. Neurobiol Dis. 2001, 8: 155-161. 10.1006/nbdi.2000.0334.PubMedGoogle Scholar
- Gillingwater TH, Haley JE, Ribchester RR, Horsburgh K: Neuroprotection after transient global cerebral ischemia in Wld(s) mutant mice. J Cereb Blood Flow Metab. 2004, 24: 62-66. 10.1097/01.WCB.0000095798.98378.34.PubMedGoogle Scholar
- Sajadi A, Schneider BL, Aebischer P: Wlds-mediated protection of dopaminergic fibers in an animal model of Parkinson disease. Curr Biol. 2004, 14: 326-330. 10.1016/S0960-9822(04)00050-8.PubMedGoogle Scholar
- Mi W, Beirowski B, Gillingwater TH, Adalbert R, Wagner D, Grumme D, Osaka H, Conforti L, Arnhold S, Addicks K, Wada K, Ribchester RR, Coleman MP: The slow Wallerian degeneration gene, Wlds, inhibits axonal spheroid pathology in gracile axonal dystrophy mice. Brain. 2005, 128 (2): 405-416. 10.1093/brain/awh368.PubMedGoogle Scholar
- Chaudhry V, Glass JD, Griffin JW: Wallerian degeneration in peripheral nerve disease. Neurol Clin. 1992, 10: 613-627.PubMedGoogle Scholar
- Joseph BS: Somatofugal events in Wallerian degeneration: a conceptual overview. Brain Res. 1973, 59: 1-18. 10.1016/0006-8993(73)90250-3.PubMedGoogle Scholar
- Lubinska L: On axoplasmic flow. Int Rev Neurobiol. 1975, 17: 241-296.PubMedGoogle Scholar
- McDonald WI: The time course of conduction failure during degeneration of a central tract. Exp Brain Res. 1972, 14: 550-556. 10.1007/BF00236596.PubMedGoogle Scholar
- Blundon JA, Sheller RA, Moehlenbruck JW, Bittner GD: Effect of temperature on long-term survival of anucleate giant axons in crayfish and goldfish. J Comp Neurol. 1990, 297: 377-391. 10.1002/cne.902970305.PubMedGoogle Scholar
- Perry VH, Brown MC, Tsao JW: The Effectiveness of the Gene Which Slows the Rate of Wallerian Degeneration in C57BL/Ola Mice Declines With Age. Eur J Neurosci. 1992, 4: 1000-1002.PubMedGoogle Scholar
- Sugioka M, Sawai H, Adachi E, Fukuda Y: Changes of compound action potentials in retrograde axonal degeneration of rat optic nerve. Exp Neurol. 1995, 132: 262-270. 10.1016/0014-4886(95)90031-4.PubMedGoogle Scholar
- Veronesi B, Boyes WK: Morphometric and electrophysiological evidence for a diameter-based rate of degeneration in the optic nerve of the rat. Exp Neurol. 1988, 101: 176-189. 10.1016/0014-4886(88)90002-7.PubMedGoogle Scholar
- Martinez AM, Canavarro S: Early myelin breakdown following sural nerve crush: a freeze-fracture study. Braz J Med Biol Res. 2000, 33: 1477-1482.PubMedGoogle Scholar
- Gold BG, Griffin JW, Price DL: Somatofugal axonal atrophy precedes development of axonal degeneration in acrylamide neuropathy. Arch Toxicol. 1992, 66: 57-66.PubMedGoogle Scholar
- Bouldin TW, Cavanagh JB: Organophosphorous neuropathy. I. A teased-fiber study of the spatio-temporal spread of axonal degeneraion. Am J Pathol. 1979, 94: 241-252.PubMed CentralPubMedGoogle Scholar
- Miledi R, Slater CR: On the degeneration of rat neuromuscular junctions after nerve section. J Physiol. 1970, 207: 507-528.PubMed CentralPubMedGoogle Scholar
- Lubinska L: Patterns of Wallerian degeneration of myelinated fibres in short and long peripheral stumps and in isolated segments of rat phrenic nerve. Interpretation of the role of axoplasmic flow of the trophic factor. Brain Res. 1982, 233: 227-240. 10.1016/0006-8993(82)91199-4.PubMedGoogle Scholar
- Chaudhry V, Cornblath DR: Wallerian degeneration in human nerves: serial electrophysiological studies. Muscle Nerve. 1992, 15: 687-693. 10.1002/mus.880150610.PubMedGoogle Scholar
- Lubinska L: Early course of Wallerian degeneration in myelinated fibres of the rat phrenic nerve. Brain Res. 1977, 130: 47-63. 10.1016/0006-8993(77)90841-1.PubMedGoogle Scholar
- George R, Griffin JW: The proximo-distal spread of axonal degeneration in the dorsal columns of the rat. J Neurocytol. 1994, 23: 657-667.PubMedGoogle Scholar
- Lunn ER, Brown MC, Perry VH: The pattern of axonal degeneration in the peripheral nervous system varies with different types of lesion. Neuroscience. 1990, 35: 157-165. 10.1016/0306-4522(90)90130-V.PubMedGoogle Scholar
- Causey G, Stratmann CJ: The spread of failure of conduction in degenerating mammalian nerve. J Physiol. 1953, 121: 215-223.PubMed CentralPubMedGoogle Scholar
- Sievers C, Platt N, Perry VH, Coleman MP, Conforti L: Neurites undergoing Wallerian degeneration show an apoptotic-like process with Annexin V positive staining and loss of mitochondrial membrane potential. Neurosci Res. 2003, 46: 161-169. 10.1016/S0168-0102(03)00039-7.PubMedGoogle Scholar
- Bendszus M, Stoll G: Caught in the act: in vivo mapping of macrophage infiltration in nerve injury by magnetic resonance imaging. J Neurosci. 2003, 23: 10892-10896.PubMedGoogle Scholar
- Sea T, Ballinger ML, Bittner GD: Cooling of peripheral myelinated axons retards Wallerian degeneration. Exp Neurol. 1995, 133: 85-95. 10.1006/exnr.1995.1010.PubMedGoogle Scholar
- Malbouisson AM, Ghabriel MN, Allt G: The non-directional pattern of axonal changes in Wallerian degeneration: a computer-aided morphometric analysis. J Anat. 1984, 139 (Pt 1): 159-174.PubMed CentralPubMedGoogle Scholar
- Beirowski B, Berek L, Adalbert R, Wagner D, Grumme DS, Addicks K, Ribchester RR, Coleman MP: Quantitative and qualitative analysis of Wallerian degeneration using restricted axonal labelling in YFP-H mice. J Neurosci Methods. 2004, 134: 23-35. 10.1016/j.jneumeth.2003.10.016.PubMedGoogle Scholar
- Feng G, Mellor RH, Bernstein M, Keller-Peck C, Nguyen QT, Wallace M, Nerbonne JM, Lichtman JW, Sanes JR: Imaging neuronal subsets in transgenic mice expressing multiple spectral variants of GFP. Neuron. 2000, 28: 41-51. 10.1016/S0896-6273(00)00084-2.PubMedGoogle Scholar
- Almenar-Queralt A, Goldstein LS: Linkers, packages and pathways: new concepts in axonal transport. Curr Opin Neurobiol. 2001, 11: 550-557. 10.1016/S0959-4388(00)00248-8.PubMedGoogle Scholar
- Campenot RB, Eng H: Protein synthesis in axons and its possible functions. J Neurocytol. 2000, 29: 793-798. 10.1023/A:1010939307434.PubMedGoogle Scholar
- Miller CC, Ackerley S, Brownlees J, Grierson AJ, Jacobsen NJ, Thornhill P: Axonal transport of neurofilaments in normal and disease states. Cell Mol Life Sci. 2002, 59: 323-330. 10.1007/s00018-002-8425-7.PubMedGoogle Scholar
- Shah JV, Cleveland DW: Slow axonal transport: fast motors in the slow lane. Curr Opin Cell Biol. 2002, 14: 58-62. 10.1016/S0955-0674(01)00294-0.PubMedGoogle Scholar
- Donat JR, Wisniewski HM: The spatio-temporal pattern of Wallerian degeneration in mammalian peripheral nerves. Brain Res. 1973, 53: 41-53. 10.1016/0006-8993(73)90765-8.PubMedGoogle Scholar
- Causey G, Palmer E: The centrifugal spread of structural change at the nodes in degenerating mammalian nerves. J Anat. 1953, 87: 185-191.PubMed CentralPubMedGoogle Scholar
- Singer M, Steinberg MC: Wallerian degeneration: a reevaluation based on transected and colchicine-poisoned nerves in the Amphibian, Triturus. Am J Anat. 1972, 133: 51-83.PubMedGoogle Scholar
- McLean WG, McKay AL, Sjostrand J: Electrophoretic analysis of axonally transported proteins in rabbit vagus nerve. J Neurobiol. 1983, 14: 227-236. 10.1002/neu.480140306.PubMedGoogle Scholar
- Whiteley SJ, Townsend J, Tomlinson DR, Brown AM: Fast orthograde axonal transport in sciatic motoneurones and nerve temperature in streptozotocin-diabetic rats. Diabetologia. 1985, 28: 847-851. 10.1007/BF00291076.PubMedGoogle Scholar
- Schlote W, Wolburg H, Wendt-Gallitelli MF: Ionic shifts in myelinated nerve fibers during early stages of Wallerian degeneration. Acta Neuropathol Suppl (Berl). 1981, 7: 31-35.Google Scholar
- Young W: Role of calcium in central nervous system injuries. J Neurotrauma. 1992, 9 (Suppl 1): S9-25.PubMedGoogle Scholar
- Glass JD, Culver DG, Levey AI, Nash NR: Very early activation of m-calpain in peripheral nerve during Wallerian degeneration. J Neurol Sci. 2002, 196: 9-20. 10.1016/S0022-510X(02)00013-8.PubMedGoogle Scholar
- Glass JD: Wallerian degeneration as a window to peripheral neuropathy. J Neurol Sci. 2004, 220: 123-124. 10.1016/j.jns.2004.03.010.PubMedGoogle Scholar
- Schumacher PA, Eubanks JH, Fehlings MG: Increased calpain I-mediated proteolysis, and preferential loss of dephosphorylated NF200, following traumatic spinal cord injury. Neuroscience. 1999, 91: 733-744. 10.1016/S0306-4522(98)00552-1.PubMedGoogle Scholar
- Wang MS, Davis AA, Culver DG, Wang Q, Powers JC, Glass JD: Calpain inhibition protects against Taxol-induced sensory neuropathy. Brain. 2004, 127: 671-679. 10.1093/brain/awh078.PubMedGoogle Scholar
- Schlaepfer WW: Calcium-induced degeneration of axoplasm in isolated segments of rat peripheral nerve. Brain Res. 1974, 69: 203-215. 10.1016/0006-8993(74)90002-X.PubMedGoogle Scholar
- Schlaepfer WW: Effects of energy deprivation on Wallerian degeneration in isolated segments of rat peripheral nerve. Brain Res. 1974, 78: 71-81. 10.1016/0006-8993(74)90354-0.PubMedGoogle Scholar
- George EB, Glass JD, Griffin JW: Axotomy-induced axonal degeneration is mediated by calcium influx through ion-specific channels. J Neurosci. 1995, 15: 6445-6452.PubMedGoogle Scholar
- Tsao JW, George EB, Griffin JW: Temperature modulation reveals three distinct stages of Wallerian degeneration. J Neurosci. 1999, 19: 4718-4726.PubMedGoogle Scholar
- LoPachin RM, Lehning EJ: Mechanism of calcium entry during axon injury and degeneration. Toxicol Appl Pharmacol. 1997, 143: 233-244. 10.1006/taap.1997.8106.PubMedGoogle Scholar
- Mata M, Staple J, Fink DJ: Changes in intra-axonal calcium distribution following nerve crush. J Neurobiol. 1986, 17: 449-467.PubMedGoogle Scholar
- Hoogeveen JF, Troost D, Wondergem J, van der Kracht AH, Haveman J: Hyperthermic injury versus crush injury in the rat sciatic nerve: a comparative functional, histopathological and morphometrical study. J Neurol Sci. 1992, 108: 55-64. 10.1016/0022-510X(92)90188-Q.PubMedGoogle Scholar
- Podhajsky RJ, Myers RR: The vascular response to nerve crush: relationship to Wallerian degeneration and regeneration. Brain Res. 1993, 623: 117-123. 10.1016/0006-8993(93)90018-I.PubMedGoogle Scholar
- Chen LE, Seaber AV, Urbaniak JR: The influence of magnitude and duration of crush load on functional recovery of the peripheral nerve. J Reconstr Microsurg. 1993, 9: 299-306.PubMedGoogle Scholar
- Haftek J, Thomas PK: Electron-microscope observations on the effects of localized crush injuries on the connective tissues of peripheral nerve. J Anat. 1968, 103: 233-243.PubMed CentralPubMedGoogle Scholar
- Bridge PM, Ball DJ, Mackinnon SE, Nakao Y, Brandt K, Hunter DA, Hertl C: Nerve crush injuries – a model for axonotmesis. Exp Neurol. 1994, 127: 284-290. 10.1006/exnr.1994.1104.PubMedGoogle Scholar
- Dyck PJ, Lais AC, Giannini C, Engelstad JK: Structural alterations of nerve during cuff compression. Proc Natl Acad Sci U S A. 1990, 87: 9828-9832.PubMed CentralPubMedGoogle Scholar
- Kitao A, Hirata H, Morita A, Yoshida T, Uchida A: Transient damage to the axonal transport system without Wallerian degeneration by acute nerve compression. Exp Neurol. 1997, 147: 248-255. 10.1006/exnr.1997.6601.PubMedGoogle Scholar
- Powell HC, Myers RR: Pathology of experimental nerve compression. Lab Invest. 1986, 55: 91-100.PubMedGoogle Scholar
- Lundborg G, Dahlin LB: The pathophysiology of nerve compression. Hand Clin. 1992, 8: 215-227.PubMedGoogle Scholar
- Whitmore AV, Lindsten T, Raff MC, Thompson CB: The proapoptotic proteins Bax and Bak are not involved in Wallerian degeneration. Cell Death Differ. 2003, 10: 260-261. 10.1038/sj.cdd.4401147.PubMedGoogle Scholar
- Giuditta A, Kaplan BB, van Minnen J, Alvarez J, Koenig E: Axonal and presynaptic protein synthesis: new insights into the biology of the neuron. Trends Neurosci. 2002, 25: 400-404. 10.1016/S0166-2236(02)02188-4.PubMedGoogle Scholar
- Gallant PE: Axonal protein synthesis and transport. J Neurocytol. 2000, 29: 779-782. 10.1023/A:1010961022455.PubMedGoogle Scholar
- Eng H, Lund K, Campenot RB: Synthesis of beta-tubulin, actin, and other proteins in axons of sympathetic neurons in compartmented cultures. J Neurosci. 1999, 19: 1-9.PubMedGoogle Scholar
- Hoffman PN, Lasek RJ: The slow component of axonal transport. Identification of major structural polypeptides of the axon and their generality among mammalian neurons. J Cell Biol. 1975, 66: 351-366. 10.1083/jcb.66.2.351.PubMedGoogle Scholar
- Smith RS, Bisby MA: Persistence of axonal transport in isolated axons of the mouse. Eur J Neurosci. 1993, 5: 1127-1135.PubMedGoogle Scholar
- Glass JD, Griffin JW: Neurofilament redistribution in transected nerves: evidence for bidirectional transport of neurofilaments. J Neurosci. 1991, 11: 3146-3154.PubMedGoogle Scholar
- Gamble HJ, JHA BD: Some effects of temperature upon the rate and progress of Wallerian degeneration in mammalian nerve fibers. J Anat. 1958, 92: 171-177.PubMed CentralPubMedGoogle Scholar
- Joseph BS, Whitlock DG: The spatio-temporal course of Wallerian degeneration within the CNS of toads (Bufo marinus) as defined by the Nauta silver method. Brain Behav Evol. 1972, 5: 1-17.PubMedGoogle Scholar
- Cancalon P: Proximodistal degeneration of C-fibers detached from their perikarya. J Cell Biol. 1983, 97: 6-14. 10.1083/jcb.97.1.6.PubMedGoogle Scholar
- Marzullo TC, Britt JM, Stavisky RC, Bittner GD: Cooling enhances in vitro survival and fusion-repair of severed axons taken from the peripheral and central nervous systems of rats. Neurosci Lett. 2002, 327: 9-12. 10.1016/S0304-3940(02)00378-6.PubMedGoogle Scholar
- Gillingwater TH, Ribchester RR: Compartmental neurodegeneration and synaptic plasticity in the Wld(s) mutant mouse. J Physiol. 2001, 534: 627-639. 10.1111/j.1469-7793.2001.00627.x.PubMed CentralPubMedGoogle Scholar
- Cavanagh JB: The 'dying back' process. A common denominator in many naturally occurring and toxic neuropathies. Arch Pathol Lab Med. 1979, 103: 659-664.PubMedGoogle Scholar
- Spencer PS, Schaumburg HH: Central-peripheral distal axonopythy – the pathology of dying-back polyneuropathies. Progress in neuropathology. Edited by: Zimmerman HM. 1976, New York: Grune and Stratton, 253-295.Google Scholar
- Fischer LR, Culver DG, Tennant P, Davis AA, Wang M, Castellano-Sanchez A, Khan J, Polak MA, Glass JD: Amyotrophic lateral sclerosis is a distal axonopathy: evidence in mice and man. Exp Neurol. 2004, 185: 232-240. 10.1016/j.expneurol.2003.10.004.PubMedGoogle Scholar
- Frei R, Motzing S, Kinkelin I, Schachner M, Koltzenburg M, Martini R: Loss of distal axons and sensory Merkel cells and features indicative of muscle denervation in hindlimbs of P0-deficient mice. J Neurosci. 1999, 19: 6058-6067.PubMedGoogle Scholar
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