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The intrinsic and synaptic responsiveness of a new realistic Purkinje cell model

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The latest discoveries on Purkinje cell (PC) physiology suggest that the mechanisms of PCs intrinsic excitability have to be revisited. Starting from available models [1], we have constructed a new PC model in Python-NEURON, which explicitly accounts for the Axon Initial Segment (AIS) [24] and a part of the axon including the first node of Ranvier (RVN). The fast Na+ channels are located in AIS, soma with initial dendrite and RVN [4]. The K+ delayed rectifier channels are located only in the soma. The Ca2+ and Ca2+-dependent K+ channels, including SK2, as well as intracellular Ca2+ dynamics have been updated [5]. The new model configuration now generates simple spike (SS) firing reproducing the experimental input-output curve[6]. SSs initiate in AIS and then back-propagate into the soma decaying sharply inside the dendritic tree. Activation of parallel fiber (pf) generates a short burst followed by a pause caused by Stellate cells. Following a complex spike (CS), SS activity is interrupted independently of the inhibitory synaptic input. Interestingly, the model can shift its state from silent to autorhythmic (configuring a bistable behavior) upon transient current injection or activation of CFs. The pf and granule cell ascending axon (aa) synapses have been modeled using a stochastic release mechanism activating AMPA synaptic receptors. The facilitation and depression profiles of pf and aa synapses faithfully reproduce the experimental data. This model provides a valuable tool to further investigate the Purkinje cell function in cerebellar network models.

References

  1. 1.

    De Schutter E, Bower JM: An active membrane model of the cerebellar Purkinje cell. I. Simulation of current clamps in slice. Journal of neurophysiology. 1994, 71: 375-400.

  2. 2.

    Palmer LM, Clark BA, Gr J, Roth A, Stuart GJ, Michael H: Initiation of simple and complex spikes in cerebellar Purkinje cells. Society. 2010, 10: 1709-1717.

  3. 3.

    Foust A, Popovic M, Zecevic D, Mccormick DA: Action Potentials Initiate in the Axon Initial Segment and Propagate through Axon Collaterals Reliably in Cerebellar Purkinje Neurons. Methods. 2010, 30: 6891-6902.

  4. 4.

    Zonta B, Desmazieres A, Rinaldi A, Tait S, Sherman DL, Nolan MF, Brophy PJ: A critical role for Neurofascin in regulating action potential initiation through maintenance of the axon initial segment. Neuron. 2011, 69: 945-56. 10.1016/j.neuron.2011.02.021.

  5. 5.

    Lorincz A, Nusser Z: Cell-type-dependent molecular composition of the axon initial segment. The Journal of neuroscience : the official journal of the Society for Neuroscience. 2008, 28: 14329-40. 10.1523/JNEUROSCI.4833-08.2008.

  6. 6.

    Anwar H, Hong S, De Schutter E: Controlling Ca2+-Activated K+ Channels with Models of Ca2+ Buffering in Purkinje Cells. The Cerebellum. 2010, 1-13.

  7. 7.

    Khaliq ZM, Gouwens NW, Raman IM: The contribution of resurgent sodium current to high-frequency firing in Purkinje neurons: an experimental and modeling study. Journal of Neuroscience. 2003, 23: 4899-4912.

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Correspondence to Stefano Masoli.

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This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Masoli, S., Solinas, S. & D'Angelo, E. The intrinsic and synaptic responsiveness of a new realistic Purkinje cell model. BMC Neurosci 14, P80 (2013) doi:10.1186/1471-2202-14-S1-P80

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Keywords

  • Purkinje Cell
  • Axon Initial Segment
  • Complex Spike
  • Bistable Behavior
  • Intrinsic Excitability