- Poster presentation
- Open Access
Spikelets and bursts in axonless retinal AII amacrine cells coupled by gap junctions
BMC Neurosciencevolume 14, Article number: P364 (2013)
AII amacrine cells play a central role in the processing of visual signals in the retina. They do not have axons but possess elaborate, ramified dendrites, through which they receive low-light input from rod bipolar cells. These dendrites also serve as output structures: chemical synapses inhibit the OFF cone-pathway and gap junctions provide input into the ON cone-pathway. Gap junctions also directly couple the AIIs to one another. In slice experiments, the AIIs are found to generate surprisingly small action potentials and can exhibit persistent bursting. In mouse rd1-mutants, which serve as a model for retinal degeneration, the death of the photoreceptors is followed by the appearance of strong rhythmicity in the spiking activity of the ganglion cells, which provide the output of the retina.
Using computational modeling we show that the small size of the action potentials, as observed in the soma, is due to the ramified morphology of the AII (Figure 1A). In fact, the modeling reveals that the spikes must originate in a single compartment that is electrotonically distant from the soma. Our modeling and subsequent electrophysiology experiments show that the bursting is due to a slow current, most likely an M-type K-conductance .
Experimentally, it has been found that the oscillations in rd1-mutant mice are abolished by the gap-junction blocker MFA . Our model suggests that the elimination of the oscillations is not due to the blocking of the gap junctions but rather by the known effects of MFA on the kinetics of the M-type K-current: MFA shifts the activation curve of the M-current to more negative potentials and slows down its deactivation . Consistent with experiments  our model shows a decrease in oscillation frequency before the oscillations are abolished with increasing MFA concentration (Figure 1B). Moreover, it generates tonic firing if the AIIs are depolarized in the presence of MFA, as has been observed experimentally .
Cembrowski MS, Logan SM, Tian M, Jia L, Li W, Kath WL, Riecke H, Singer JH: A single, distal dendritic location underlies action potential-like spiking in an axonless retinal interneuron. Cell Reports. 2012, 1: 155-166. 10.1016/j.celrep.2011.12.006.
Trenholm W, Borowska J, Zhang JC, Hoggarth A, Johnson K, Barnes S, Lewis T, Awatramani G: Intrinsic oscillatory activity arising within the electrically-coupled AII Amacrine/on cone bipolar cell network is driven by voltage-gated Na+ channels. J Physiol. 2012, 590: 2501-2517.
Peretz A, Degani N, Nachman R, Uziyel Y, Gibor G, Shabat D, Attali B: Meclofenamic acid and diclofenac, novel templates of KCNQ2/Q3 potassium channel openers, depress cortical neuron activity and exhibit anticonvulsant properties. Molecul Pharmacol. 2005, 67: 1053-1066. 10.1124/mol.104.007112.
Menzler J, Zeck G: Network oscillations in rod-degenerated mouse retinas. J Neurosci. 2011, 31: 2280-2291. 10.1523/JNEUROSCI.4238-10.2011.