Phosphorylated human α-Synuclein is targeted to nuclei but is undetectable in neurites. Immunoreactivity for transgenic hα-Syn (green channel) is evident in a subset of pyramidal neurons (A, C) in the hippocampal area CA3 and (B) in the dorsolateral neocortex (arrowheads); in either area the transgenic isoform is targeted to both somata and neurites. Using an antibody against human protein phosphorylated at S129 (phα-Syn, red channel), immunoreactivity is strong in most neurons that are positive for hα-Syn but is spatially restricted to neuronal nuclei, as is evident from co-localization with DAPI (arrowheads) (C). Scale bar: 150 μm (A, B), 100 μm (C). Abbreviations: cornu ammonis areas 1–3 (CA1-3), stratum oriens (so), stratum pyramidale (sp), stratum lucidum (sl), stratum radiatum (sr), stratum lacunosum moleculare (slm), white matter (wm), cortical layer V and VI (V and VI).