Modeling the chemical dynamics of chloride ion indicators
© Redford et al; licensee BioMed Central Ltd. 2012
Published: 16 July 2012
Fluorescent indicators have shown promise as a relatively non-invasive probe to measure cytosolic ion concentrations in brain slices and neuron cultures using microscopy imaging. Most fluorescent ion indicators bind selectively with a certain ion in solution causing a decrease in fluorescence in a process known as quenching. Under steady state conditions, a fluorescence measurement, made at a specific point and time, is directly related to the local ion concentration at the same point and time, typically via the Stern-Volmer relationship. However, this is usually no longer true under the dynamic conditions inside a cell when transmembrane currents are active.
In our research, we are interested in measuring chloride (Cl-) channel currents because of their implication in substance abuse mechanisms. Useful probes have been found in Cl- sensitive dyes, such as N-(6-methoxyquinolyl) acetoethyl ester (MQAE) , and 6-methoxy-N-ethylquinolinium (MEQ) , and variants of the Yellow Fluorescent Protein (YFP) useful as a Cl- biosensor in dendritic compartments .
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