Figure 5
Differential effects of C/EBP knockdown on the distal, proximal, and intact neurites following axotomy. The length of the distal, proximal, or intact neurite of injured cells was measured at three time points (10 hrs, 24 hrs, 36 hrs) after control or C/EBP siRNA treatment, which was given immediately after axotomy (t = 0). (A) The distal neurite. The net changes in the C/EBP siRNA #1 or #2 treated cells were significantly reduced as compared to that in the control CM or control siRNA treated cells (n = 14; ANOVA: at 10 hrs, F(3,52) = 14.4, p < 0.05; at 24 hrs, F(3,52) = 20.1, P < 0.05; at 36 hrs, F(3,52) = 29.7, p < 0.05). (B) The proximal neurite. A significant reduction in outgrowth was observed only at 36 hrs after axotomy in the C/EBP siRNA #1 or #2 treated cells as compared to control CM and control siRNA treated cells (n = 14; ANOVA: F(3,52) = 5.1, p < 0.05). (C) The intact neurite. A significant reduction in outgrowth was only observed at 36 hrs in the C/EBP siRNA #1 or #2 treated cells as compared to control CM and control siRNA treated cells (n = 11; ANOVA: F(3,40) = 6.5, p < 0.05). (D) Relative gene expression level of C/EBP vs GAPDH was significantly reduced in the C/EBP siRNA #1 (63.34 ± 8.8%) and #2 (56.25 ± 9.4%) (n = 5, ANOVA: F(2,12) = 5.7, p < 0.05) groups as compared to control siRNA group. All data are presented as mean ± S.E.M.. * indicates p < 0.05.