Compensating the effect of dendritic diameters on calcium transients: a modeling study
BMC Neuroscience volume 12, Article number: P60 (2011)
Intracellular Ca2+ does not only play a crucial role in the physiological interaction between the Ca2+ channels and Ca2+ activated K+ channels, it also serves as an important cellular messenger in signaling pathways. Therefore, accurate representation of intracellular calcium concentration is required in biophysically plausible models. Most commonly, intracellular calcium is modeled in morphologically realistic neuron models using single [1–3] or double exponential decaying pools  where Ca2+ concentration is computed in a submembrane shell only. These models are rather insensitive to the diameter of a compartment but fail to simulate interaction between Ca2+ channels and Ca2+ activated K channels occurring at multiple time scales. A more comprehensive and biophysically realistic solution is to use a detailed calcium dynamics model [5, 6] with buffers, pump and diffusion. When we used detailed Ca2+ dynamics model with a detailed morphology of a Purkinje cell, we discovered large gradients of Ca2+ levels in neighboring segments with different diameters even in the present of lateral diffusion. The peak Ca2+ concentration showed a close to linear inverse relationship to diameter of the compartment. We deem such pronounced gradients of Ca2+ as unphysiological and suggest that there should be a regulatory mechanism to compensate the effect of local dendritic geometry on buffered calcium transients.
In this study, we used a detailed calcium dynamics model  in a piece of dendrite where diameters of segments were varied to study different combinations of diameter changes (small to large variation). All the simulations were run using STEPS  with a fine resolution mesh to allow accurate modeling of diffusion.
Assuming a uniform calcium channel density for influx and uniformly distributed calcium buffers and pumps, difference in diameters of segments gave rise to large gradients of calcium transients. We investigated several possible mechanisms that could compensate the effect of local dendritic geometry on gradients of intracellular levels. These regulatory mechanisms included scaling of channel densities, scaling of pump densities, scaling of buffer concentrations and subcellular localization of buffers. Further, we also investigated combination of these regulatory mechanisms to compensate for the differences in peak amplitudes of calcium transients.
Our results suggest that the effect of local dendritic geometry on intracellular calcium levels can be partially compensated by each of the regulatory mechanisms investigated and can be sufficiently compensated by combination of those regulatory mechanisms. Therefore, on the basis of our modeling work, we propose a quantitative physiological investigation of the suggested mechanisms.
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Anwar, H., De Schutter, E. Compensating the effect of dendritic diameters on calcium transients: a modeling study. BMC Neurosci 12 (Suppl 1), P60 (2011). https://doi.org/10.1186/1471-2202-12-S1-P60