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Modeling UBC intrinsic excitability
BMC Neuroscience volume 12, Article number: P322 (2011)
Unipolar brush cells (UBCs) are excitatory glutamatergic interneurons of the cerebellar granular layer receiving both primary and secondary vestibular inputs through mossy fibers (excitatory input) and Golgi axon (inhibitory input). The brush like structure of the dendrite allows to form a giant synapses in the glomerulus and to produce an all or none post synaptic response with short delay and protracted kinetics. The excitable response of UBCs can be either a tonic discharge or a high-frequency burst of action potentials. When injected with progressively increasing depolarizing currents from a negative membrane potential, the UBC generates a burst sustained by a calcium spike (Figure 1, +20pA) and then a protracted discharge with shorter latency and spike frequency adaption (Figure 1, +25pA). The intrinsic excitability of UBCs is determined by an H current and by Low Voltage activated and High Voltage activated calcium currents [2, 3]. Fast inactivating T-type Calcium channels generate low-threshold spikes and L-type Calcium channel sustain tonic firing. The H current (activated between -60mV and -80mV) produces a slow hyperpolarization characterized by a “sag” in response to a hyperpolarizing step (Figure 1, -16pA) and an afterhyperpolarization at the end of a depolarizing step.
Here we present a biologically realistic multi-compartmental mathematical model of the UBC realized with the NEURON simulator. According to literature [1–4], ionic channels are distributed among compartments (soma, dendrite, and axon). The model can reproduce the excitable properties of UBCs in current-clamp and voltage-clamp modes. Attempts at modeling the response to mossy fiber inputs are ongoing. This model confirms the primary role of the aforementioned currents in UBC’s electroresponsiveness. The model will also be a valuable tool for investigating the UBC’s function in the cerebellar network.
Rossi DJ, Altford S, Mugnaini E, Slater NT: Properties of transmission at a giant glutamatergic synapse in cerebellum: the mossy fiber-unipolar brush cell synapse. J. Neurophysiol. 1995, 74 (1): 24-42.
Diana MA, Otsu Y, Maton G, Collin T, Chat M, Dieudonné S: T-type and L-type Ca2+ conductances define and encode the bimodal firing pattern of vestibulocerebellar unipolar brush cells. J Neurosci. 2007, 27 (14): 3823-3838. 10.1523/JNEUROSCI.4719-06.2007.
Russo MJ, Yau HJ, Nunzu MG, Mugnaini E, Martina M: Dynamic metabotropic control of intrinsic fring in cerebellar unipolar brush cells. J Neurophysiol. 2008, 100 (6): 3351-3360. 10.1152/jn.90533.2008.
Ruigrok TJH, Henshroek RA, Simpson JI: Spontaneous activity signatures of morphologically identified interneurons in the vestibulocerebellum. The Journal of Neuroscience. 2011, 31 (2): 712-724. 10.1523/JNEUROSCI.1959-10.2011.
This work was supported by the EU projects CEREBNET and REALNET to ED. SS is recipient of a grant from CEREBNET.
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Subramaniyam, S., Perin, P., Solinas, S. et al. Modeling UBC intrinsic excitability. BMC Neurosci 12 (Suppl 1), P322 (2011). https://doi.org/10.1186/1471-2202-12-S1-P322
- Mossy Fiber
- Brush Cell
- Tonic Firing
- Negative Membrane Potential
- Intrinsic Excitability