Volume 12 Supplement 1
Analysis of simultaneous multielectrode recordings with 4,096 channels: changing dynamics of spontaneous activity in the developing retina
© Hennig et al; licensee BioMed Central Ltd. 2011
Published: 18 July 2011
Our current understanding of the dynamics of neural circuits is limited by the poor resolution of multi-neuron recordings from large neural populations, which largely prevents the experimental verification of theoretical models and predictions. It is, for instance, difficult to distinguish between different potential classes of network architecture, such as feed-forward or recurrent networks, on the basis of simultaneous recordings from just tens of neurons. Recent advances in electronics have now made it possible to simultaneously record from thousands of neurons. Here, we used the Active Pixel Sensor (APS) multielectrode array (MEA) [1, 2], consisting of 4,096 electrodes recording at near cellular resolution (21μm electrode diameter, 42μm centre-to-centre separation, arranged on a 64x64 lattice), to record spontaneous neural activity in the developing neonatal mouse retina in vitro. This activity takes the form of spontaneous propagating waves, which can be recorded in the ganglion cell layer [3, 4]. This spontaneous activity occurs before the retina responsive to visual stimulation, and is thought to provide cues instructive for the wiring of visual connections. So far, retinal waves have been investigated with MEAs ranging from 60 [reviewed in ref. 4] to 512 electrodes , and with Ca2+ imaging [reviewed in refs. 3 and 4]. These previous studies were, therefore, either limited by the spatial resolution of the MEA, or by the slow temporal response of Ca2+ indicators. While it is well established that the properties of retinal waves change during development, so far wave dynamics have been extrapolated from these limited data sets. Here, we provide a complete characterisation of the dynamics of retinal waves during the first two postnatal weeks, and present several methods for the analysis of such activity patterns. In the mammalian retina, the earliest waves propagate through gap junctions (Stage I, prenatal in mouse), followed by lateral propagation between cholinergic starburst amacrine cells (Stage II) and finally by activity that depends on glutamatergic synaptic transmission (Stage III). Consistent with an earlier analysis of 60 channel MEA recordings , we found that Stage II waves exhibit a high degree of randomness with respect to initiation points, trajectories , sizes and durations. Stage III waves, on the other hand, were significantly faster and they were more restricted spatially, following several clear repetitive, non-random propagation patterns that appear to tile the retina, mostly starting from the periphery and propagating towards the centre. This latter effect can not be identified in recordings with conventional 60 channel MEAs, underscoring the importance of probing and analysing neural circuits at a near-cellular resolution.
This work was funded by MRC Fellowship G0900425 (MHH), BBSRC Grant BB/H023607/1 (MHH, SJE, ES), EPSRC Grant EP/E002331/1 (CARMEN) (SJE, ES) and IDEA, FP6-NEST Grant 516432 (AM, MG, LB).
- Imfeld K, Neukom S, Maccione A, Bornat Y, Martinoia S, Farine PA, Koudelka-Hep M, Berdondini L: Large-scale, high-resolution data acquisition system for extracellular recording of electrophysiological activity. IEEE Trans Biomed Eng. 2008, 55: 2064-2073. 10.1109/TBME.2008.919139.View ArticlePubMedGoogle Scholar
- Berdondini L, Imfeld K, Maccione A, Tedesco M, Neukom S, Koudelka-Hep M, Martinoia S: Active pixel sensor array for high spatio-temporal resolution electrophysiological recordings from single cell to large scale neuronal networks. Lab Chip. 2009, 9: 2644-2651. 10.1039/b907394a.View ArticlePubMedGoogle Scholar
- Sernagor E, Eglen SJ, Wong ROL: Development of retinal ganglion cell structure and function. Prog Retin Eye Res. 2001, 20: 139-174. 10.1016/S1350-9462(00)00024-0.View ArticlePubMedGoogle Scholar
- Torborg CL, Feller MB: Spontaneous patterned retinal activity. Prog Neurobiol. 2005, 76: 213-235. 10.1016/j.pneurobio.2005.09.002.View ArticlePubMedGoogle Scholar
- Stafford BK, Sher A, Litke AM, Feldheim DA: Spatial-temporal patterns of retinal waves underlying activity-dependent refinement of retinofugal projections. Neuron. 2009, 64: 200-212. 10.1016/j.neuron.2009.09.021.PubMed CentralView ArticlePubMedGoogle Scholar
- Hennig MH, Adams C, Willshaw D, Sernagor E: Early-stage waves in the retinal network emerge close to a critical state transition between local and global functional connectivity. J Neurosci. 2009, 29: 1077-1086. 10.1523/JNEUROSCI.4880-08.2009.View ArticlePubMedGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.