- Research article
- Open Access
The p75 neurotrophin receptor localization in blood-CSF barrier: expression in choroid plexus epithelium
© Spuch and Carro; licensee BioMed Central Ltd. 2011
Received: 10 February 2011
Accepted: 11 May 2011
Published: 11 May 2011
The presence of neurotrophins and their receptors Trk family has been reported in the choroid plexus. High levels of Nerve Growth Factor (NGF), Neurotrophin-4 (NT-4) and TrkB receptor were detected, while nothing was know about p75 neurotrophin receptor (p75NTR) in the choroid plexus epithelial cells. In neurons, p75NTR receptor has a dual function: promoting survival together with TrkA in response to NGF, and inducing apoptotic signaling through p75NTR. We postulated that p75NTR may also affect the survival pathways in the choroid plexus and also undergoes regulated proteolysis with metalloproteases.
Here, we demonstrated the presence of p75NTR receptor in the choroid plexus epithelial cells. The p75NTR receptor would be involved in cell death mechanisms and in the damaged induced by amyloid beta (Aβ) in the choroid plexus and finally, we propose an essential role of p75NTR in the Aβ transcytosis through out choroid plexus barrier.
The presence analysis reveals the new localization of p75NTR in the choroid plexus and, the distribution mainly in the cytoplasm and cerebrospinal fluid (CSF) side of the epithelial cells. We propose that p75NTR receptor plays a role in the survival pathways and Aβ-induced cell death. These data suggest that p75NTR dysfunction play an important role in the pathogenesis of brain diseases. The importance and novelty of this expression expands a new role of p75NTR.
The choroid plexus, located in the cerebral ventricles, is a highly vascularised tissue, in which blood microvessels are enclosed by a single layer of cubical epithelial cells . Choroid plexus epithelial cells are closely connected to each other by tight junctions and constitute the structural basis of the blood-CSF barrier . The barrier function can be subjected to modulation and thereby regulates the entry of physiologically important substances. However, choroid plexus epithelial cells are also an important target organ for polypeptides, with capacity to produce and secrete numerous biologically active neurotrophic factors into the CSF. In mammalian, brain CSF is produced by the choroid plexus, which not only regulates homeostasis in central nervous system (CNS), but also participates in neurohumoral brain modulation as well as in neuroimmune interaction. Several peptides are shown to be actively transported by the choroidal epithelial cells to the CSF and most of the transported hormones evidently have, at least, a hypothalamic destination. These peptides circulate throughout the brain and spinal cord, maintaining neuronal networks and associated cells .
Studies in the past few years have promoted insight into the molecular structure and function of the choroid plexus. Even modest changes in the choroid plexus can have far reaching effects and changes in the anatomy and physiology of the choroid plexus, and have been linked to several neurological disorders such as Alzheimer's disease or multiple sclerosis. Given a widely postulated role in neuronal cell survival, the p75NTR is also though to be associated with many neurodegenerative diseases.
The choroid plexus is involved in a variety of neurological disorders, including neurodegenerative, inflammatory, infectious, traumatic, neoplastic, and systemic diseases. It is well known that Aβ is accumulated in the choroid plexus of Alzheimer's disease patients ; there is also evidence that circulating Insulin-Like Growth Factor-I (IGF-I) participates in brain Aβ clearance by modulating choroid plexus function . It has been published that substances, such as transthyrretin (TTR) or apolipoprotein J (ApoJ), are produced by the choroid plexus and secreted into the CSF . In multiple sclerosis and in animal models of multiple sclerosis, the choroid plexus is the main route of leukocyte entry into the brain .
Many studies have demonstrated the presence of proteins and mRNA for a large number of cytokines, growth factors and hormones in the choroid plexus, for example: interleukin-1β , interleukin-6 , Tumor Necrosis Factor (TNF)-α , IGF-I , NGF , IGF-II , Transforming Growth Factor (TGF)-α , TGF-β , Vascular Endothelial Growth Factor (VEGF) , transferrin , TTR [6, 18], gelsolin  and vasopressin . Most of these substances have their own receptors in the choroid plexus . The production and secretion of all these substances and their receptors are strongly associated with the health of central nervous system (CNS).
The mammalian neurotrophins comprise a family of related secreted factors required for differentiation, survival, development, and death of specific populations of neurons and non-neuronal cells. The effects of the neurotrophins (NGF, Brain-Derived Neurotrophin Factor (BDNF), NT-3, NT-4) are mediated by binding to TrkA, TrkB and TrkC receptor tyrosine kinases and to the p75NTR [22, 23]. The Trk receptors play critical roles in mediating neuronal survival, growth and synaptic function . The p75NTR serves as a receptor for the four mentioned neurotrophins and it is an important component of distinct cell surface signaling platforms, which induce apoptosis and neuronal growth inhibition .
The presence of the neurotrophins and their receptors has been investigated in the choroid plexus of rats and humans. The choroid plexus contains high levels of NGF, NT-4, and TrkB, and low levels of NT-3 and BDNF, while TrkA and TrkC levels remain undetectable .
Regarding the neurotrophins receptors in the choroid plexus, the expression of p75NTR has not been fully investigated. The present research shows the distribution of p75NTR in the epithelial cells of the choroid plexus and its possible role in the normal transportation of molecules of the blood-CSF-barrier. The importance and novelty of this expression expands a new role of p75NTR.
1. The p75 neurotrophin receptor is expressed in the epithelial cells from choroid plexus
2. Proteolytic processing of p75NTR by amyloid-β (Aβ) in choroid plexus
Aβ1-40 aggregates of undetermined structure have been reported to be death-inducing ligands of p75NTR . Although Aβ is also known to bind many other targets, such as megalin, the most important multicargo receptor in the choroid plexus . It has been known for many years that p75NTR undergoes ectodomain shedding. Ectodomain cleavage of full length p75NTR releases the extracellular domain from the membrane bound C-terminal fragment (CTF) ≈24 KDa. The CTF is subsequently cleaved by γ-secretase to give rise to the soluble p75NTR intracellular domain (ICD) ≈19 KDa .
To confirm that the p75NTR-ICD does indeed accumulate in the nucleus in response to Aβ1-40, nuclear and cytoplasmic extracts were subjected to Western blotting with a p75NTR-ICD specific antibody (9992). A 19 KDa band, characterized as p75NTR-ICD fragment , was enriched in nuclear extracts from primary culture cells from choroid plexus exposed to Aβ1-40 for 15, 30 and 60 minutes (Figure 2C).
3. p75NTR receptor involved in Aβ transport in the choroid plexus
4. The p75NTR receptor is implicated in cell death induced by Aβ
The p75NTR receptor is a transmembrane protein that binds all neurotrophins and has multiple functions in the nervous system, where it is widely expressed during developmental stages of life in neurons and also in a variety of glial populations. Expression of p75NTR receptor can increase in pathological states related to neural cell death. In choroid plexus only transcripts coding for the TrkB molecule have been described . Our data clearly demonstrate that p75NTR receptor is also expressed in the choroid plexus and is mainly located towards the apical membrane in the epithelial cells. In Madine-Darby canine kidney (MDCK) cells transfected with the plasmid p75NTR receptor, the expression is restricted to the apical domain . Several studies demonstrated that p75NTR participates in more diverse biological events including neuronal cell death, migration and axonal elongation. In the present study we have suggested the role of p75NTR receptor in the epithelial cells from choroid plexus and it seems to be involved in the damage and death of cells induced by Aβ.
It is well described in neurons and different cells lines that p75NTR receptor undergoes sequential proteolytic cleavage by α-secretase and γ-secretase activities, releasing its ICD into cytoplasm, which is in a manner analogous to the cleavage-dependent signalling pathway of Notch and APP . Although neurotrophins did not regulate p75NTR processing, the α-secretase and γ-secretase mediated cleavage of p75NTR is modulated by TrkA and TrkB receptors  and the choroid plexus epithelial cells were able to express TrkB receptors . In this context, we have presented evidence that the predicted ICD of p75NTR was detectable by Western blot analysis and immunostaining after treatment with Aβ, and this fragment was translocated to the nucleus. The ICD of p75NTR was unstable in the choroid plexus; we therefore attempted to detect it by inhibiting a proteasomal pathway that had been previously shown to mediate degradation of Notch-ICD or p75-ICD in neurons . We have not evidence about the function of ICD nuclear accumulation in choroid plexus, however, there were many evidences that p75NTR-ICD have nuclear functions , such as apoptosis , transcriptional and cell cycle regulations . Numerous proteins interact with p75NTR-ICD to activate different pathways (NF-kappaB, Akt or JNK) and some of them have been associated with the translocation to the nucleus and the induction of apoptosis . However we have not evidence that some of these adaptors, such as NRIF, TRAFs, SC1, MAGE, RIP2, are expressed in choroid plexus. It remains to be determined whether adaptor proteins of p75NTR are also expressed in choroid plexus and can interact with p75NTR. The identification of p75NTR interactors and signaling pathways will spark new directions in blood-CSF-barrier research and will provide better understanding of this enigmatic receptor.
The choroid plexus play pivotal roles in basic aspects of neural function including maintaining the extracellular milieu of the brain by actively modulating chemical exchange between the CSF and brain parenchyma. In this context, it is well known the role of megalin, a multifunctional endocytic receptor. Megalin is a multicargo transmembrane protein with a large extracellular domain containing multiple binding sites for its numerous ligands. Megalin is the major receptor for the uptake of Aβ complexed with different proteins, such as TTR, clusterin, ApoE, albumin [39, 40]. Based on previous observations, megalin mediated clearance mechanisms of Aβ have been proposed to play a crucial role in the elimination of Aβ from the brain through the blood-CSF-barrier into the periphery [27, 41]. To investigate the possible role of p75NTR receptor in an artificial model of blood-CSF-barrier, we corroborated the normal Aβ transport from CSF to blood; however, when we inactivated the biological activity of p75NTR we demonstrated that Aβ transcytosis was completely blocked. These results suggest that p75NTR receptor could be modulating megalin activity in the blood-CSF-barrier, and modifying the Aβ clearance. The precise mechanism as to how the p75NTR modified megalin activity remains elusive.
Our findings have led to the suggestion that the p75NTR signaling in choroid plexus might be determined by the type of co-receptor involved in the complex. Our studies revealed that Aβ could be able to engage a functional interaction between p75NTR and megalin. Further, TrkB expression was detected in choroid plexus , and in neurons it was well know to engage functional interaction between p75NTR and TrkB . The mechanism of TrkB and p75NTR in choroid plexus remains elusive and needs more experiments; however we suggest that the possible interaction between p75NTR, TrkB and megalin will provide new directions in the p75NTR signaling and better understanding in the blood-CSF-barrier research.
The mechanism underlying this phenomenon and its relevance to neurodegenerative diseases is unclear. Therefore, it is reliable to speculate that p75NTR receptor could modify TTR expression and release it to CSF. Previous studies confirmed by data from our laboratory suggested that TTR is reduced in CSF samples from subjects with Alzheimer's disease and frontotemporal dementia . Experiments investigating the response of choroid plexus TTR synthesis after inhibition of p75NTR biological activity produced an increase of TTR synthesis; alternatively when we investigated the inhibition of p75NTR biological activity in an in vitro model of blood-CSF-barrier, we confirmed a strong release of TTR (upper chamber). It is well established in our laboratory that Aβ induced cell death in choroid plexus epithelial cells . We confirmed the implication of p75NTR receptor in Aβ-induced cell death by blocking the p75NTR biological activity. These experiments were corroborated when we over-expressed p75NTR receptor in primary culture of choroid plexus epithelial cells increasing the expression of caspase-3 and caspase-9, and cleaved of caspase-3. Dietrich's study shows Aβ accumulation in choroid plexus in different stages of Alzheimer's disease  and induced oxidative stress and mitochondrial alterations . These results showed the impact of p75NTR receptor in the survival and protection of epithelial cells from choroid plexus and confirmed the inhibition of p75NTR biological activity such as a protective mechanism in neurodegenerative diseases, but further studies using animal models will be required to confirm this hypothesis.
In summary, our results have been revealed p75NTR expression in epithelial choroid plexus, mainly localized in the apical membrane in contact with CSF side. Indeed, p75NTR plays an important role in cell surface platforms to induce neuronal apoptosis . In our context we suggest that p75NTR is also regulating the survival/apoptosis pathways and the Aβ-induced damage. The mechanism of TrkB and p75NTR in choroid plexus remains elusive and needs more experiments, although we suggest that p75NTR and TrkB could interact with megalin providing new directions in the p75NTR signaling and better understanding in the blood-CSF-barrier research.
Primary cultures from choroid plexus epithelial cells from P3-P5 Wistar rats were prepared as described previously . Cells were grown to confluence for 5-7 days and serum starved for 2 hours. Cultures were maintained at 37°C in a humidified atmosphere containing 5% CO2, and cultivated for 7 days prior to experimentation. Human analogue peptides corresponding to Aβ1-40 and scrambled Aβ1-40 (AnaSpec, Inc.) were added. 24 hours after stimulation, cells were either fixed for immunocytochemical analysis or homogenized for immunoblot determination. For ICD p75NTR detection primary cultures cells proteasome inhibitor epoxomycin (1 μM) (Sigma) was applied 1.5 hours prior to Aβ treatment.
A double-chamber choroid plexus epithelial cell-culture system mimicking the blood-CSF interface was used for in vitro studies, as described previously .
Thereafter, cells were incubated another 24 hours with excess of p75NTR (9651) antibody (1:50) (gifted from Dr. M. Chao and Dr. B. Carter). The antibody was added to the upper and lower chamber and 24 hours later the upper chambers were treated with Aβ1-40 (5 μg/mL). Twenty-four hours later, lower chamber medium was collected and content of Aβ and TTR was determined by immunoblotting (see below). Treatments were done in triplicate wells per experiment.
All animals were handled and cared for in accordance with European Community Council Directive (86/609/EEC). Animals were perfused transcardially with saline buffer and 4% paraformaldehyde in 0.1 M phosphate buffer, pH 7.4, for immunohistochemical analysis.
For Western blot analysis cultures cells were washed once with ice-cold PBS and lysed in PIK buffer (150 mM NaCl, 20 mM TrisHCl pH 7.4, 1% NP40 and protease inhibitors: 1 μg/mL aprotinin, 1 μg/mL leupeptin and 1 μg/mL phenylmethylsulfonyl fluoride, PMSF). For cell fractionation cells were lysed with C buffer (10 mM HEPES, 60 mM KCl, 1 mM EDTA, 0.075% Triton X100, 1 mM DTT with protease inhibitors. The pellet nuclei was obtained by centrifugation 325 g, 4 minutes and resuspended in NB buffer (20 mM TrisHCl pH 8, 420 mM NaCl, 15 mM MgCl2, 0.2 mM EDTA, 25% Glicerol and protease inhibitors). Thereafter, the nuclei supernatant were obtained after centrifugation 9000 g, 10 minutes. After running the samples in acrylamide gels, proteins were transferred (immobilon, Bio-Rad) and membranes were incubated with the corresponding primary at 4°C overnight. Afterwards, membranes were washed and incubated with secondary antibodies. Membranes were washed several times with Tween-TBS and developed with ECL plus (Amersham). Western blot membranes were re-blotted with unrelated proteins (α-actin) as an internal standard and normalized for protein load. Densitometric analysis was performed using ImageJ software (NIH Image). A representative blot is shown from a total of at lest three independent experiments.
For immunoprecipitation, cells were lysed with PIK buffer and centrifuged at 11000 g for 15 minutes. Supernatants were incubated with primary antibody overnight. Protein A-agarose (Amersham) was added to the antigen-antibody mixture and incubated with gentle agitation 4 hours. The immunoprecipitate was washed several times with the same PIK buffer, resuspended in SDS loading buffer and analyzed by western blot.
For cell death quantification, after incubation for 24 h with Aβ40 (5 mg/ml), choroid plexus epithelial cells in vitro were measured with Cell Death Detection ELISAPLUS kit (Roche Diagnostics) as described previously .
Immunocytochemistry was performed with primary cultures from choroid plexus plated on 20 mm coverslips and fixed. Rat and human choroid plexus were also fixed with 4% paraformaldehyde and processed for histochemical analysis. Coverslips and choroid plexus sections were blocked with 5% BSA and incubated overnight at 4°C with the respective antibody in PB containing 0.5% BSA and 0.1 Triton X100. After several washes in PB, sections were incubated with Alexa-coupled secondary antibody in the same PB buffer. Omission of primary antibody was used as control. Confocal analysis was performed in a Leica confocal microscope. Human brain tissue was obtained from the Neuropathology Institute, anatomy pathology service, IDIBELL. Hospital "Universitario de Bellvitge" (Barcelona, Spain).
The following antibodies were used: rabbit polyclonal anti-p75NTR against extracellular domain (9651) and intracellular region (9992) gifted from Dr. M. Chao and Dr. B. Carter. Mouse monoclonal anti-α-actin (Sigma), mouse monoclonal anti-β-amyloid (MBL), rabbit polyclonal anti-transtyrretin (Santa Cruz), mouse monoclonal anti-caspase 3 and 9 (Cell Signalling), goat polyclonal anti megalin (Santa Cruz). All alexa fluor antibodies were purchased from Invitrogen.
We thank Bruce Carter (Vanderbilt University Medical Center, Nashville, TN) and Moses Chao (Skirball Institute, New York, NY) for p75-ICD and p75-ECD antibodies. We also thank Mark Bothwell (University of Washington, Seattle) for expert comments and revision of the manuscript. Also, we would like to thank to Tania Vazquez for editorial assistance. This work was supported by Grants from Fondo de Investigacion Sanitaria (FIS) (CP04/00179, PI060155), Fundación Investigación Médica Mutua Madrileña (2006.125), (CP04/00011, PI050379) and Xunta de Galicia (INCITE2009, 09CSA051905PR) and "Isidro Parga Pondal" programme. The authors would like to thank to Brain Bank for Neurological Research (Universidad Complutense, Madrid, Spain) and the "Banco de Tejidos Biológicos de Vigo, BTN-Vigo" (Neurological Tissues Biobank of Vigo), which is part of the CHUVI-Biobank, recently integrated into the Spanish Cooperative Health Research Thematic Network on Biobanks (RETICS-Biobancos, Code RD09/0076/00011) for the human choroid plexus samples.
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