Skip to main content

Advertisement

What makes DA neurons tick? Role of Ca2+ sources and inward currents in setting the regular pacemaking of dopaminergic neurons

Article metrics

  • 1117 Accesses

Dopamine (DA) neurons show regular, pacemaking firing in the range 1-8 Hz in in vitro preparations. The currents involved in this pacemaking behavior are not fully understood. The slow oscillatory potential (SOP), evident after the application of TTX, is known to depend on the mutual interaction between L-type Ca2+ and small conductance (SK) K+ currents. The SOP oscillates in a frequency range similar to the pacemaking firing rate and it is assumed to be responsible for determining this pacemaking frequency [1]. However, recent results indicate that the SOP’s frequency is not correlated with spontaneous pacemaking frequency [2, 3]. Furthermore there is experimental evidence indicating that pacemaking does not require the influx of Ca2+ through L-type Ca2+ channels [4, 5]. Figure 1A illustrates this last point from a modeling approach. The black trace corresponds to the spontaneous firing of the model DA neuron and in blue the SOP. After simulated block of L-type current no activity is observed (green) but this is recovered (red) by injecting a virtual L-type current into the soma (cf. [5]). Figure 1B on the other hand illustrates the possible role of the fast Na+ current in reestablishing pacemaking. Black and blue traces are similar to those in panel A; however pacemaking is reestablished by shifting the Na+ activation curve in a hyperpolarization direction (green). We explore the role of additional inward and outward currents, particularly focusing on other Ca2+ currents (e.g. T, N), also the release of Ca2+ from intracellular stores in establishing pacemaking.

Figure 1
figure1

Pacemaking in model DA neuron in the absence of Ca2+ entry through L-type Ca2+ channels.

References

  1. 1.

    Wilson CF, Callaway JC: Coupled oscillator model of the dopaminergic neuron of the substantia nigra. J Neurophysiol. 2000, 83 (5): 3084-3100.

  2. 2.

    Guzman JN, Sanchez-Padilla J, Chan CS, Surmeier DJ: Robust pacemaking in substantia nigra dopaminergic neurons. J Neurosci. 2009, 29 (35): 11011-11019. 10.1523/JNEUROSCI.2519-09.2009.

  3. 3.

    Kuznetsova AY, Huertas MA, Kuznetsov AS, Paladini CA, Canavier C: Regulation of firing frequency in a computational model of a midbrain dopaminergic neuron. J Comput Neurosci.

  4. 4.

    Chan CS, Guzman JN, Ilijic E, Mercer JN, Rick C, Tkatch T, Meredith GE, Surmeier DJ: 'Rejuvenation' protects neurons in mouse models of Parkinson's disease. Nature. 2007, 447 (7148): 1081-1086. 10.1038/nature05865.

  5. 5.

    Putzier I, Kullmann PH, Horn JP, Levitan ES: Cav1.3 channel voltage dependence, not Ca2+ selectivity, drives pacemaker activity and amplifies bursts in nigral dopamine neurons. J Neurosci. 2009, 29 (49): 15414-15419. 10.1523/JNEUROSCI.4742-09.2009.

Download references

Acknowledgements

This work is supported by NIH grant NS 61097 to Carmen C Canavier.

Author information

Correspondence to Marco A Huertas.

Rights and permissions

Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Reprints and Permissions

About this article

Cite this article

Huertas, M.A., Canavier, C.C. What makes DA neurons tick? Role of Ca2+ sources and inward currents in setting the regular pacemaking of dopaminergic neurons. BMC Neurosci 11, P24 (2010) doi:10.1186/1471-2202-11-S1-P24

Download citation

Keywords

  • Dopamine
  • Recent Result
  • Firing Rate
  • Dopaminergic Neuron
  • Mutual Interaction