Real-time activity-dependent drug microinjection
© Chamorro et al; licensee BioMed Central Ltd. 2009
Published: 13 July 2009
Real-time (RT) software technology has an enormous potential to precisely control the spatio-temporal aspects of a stimulus and to build activity-dependent stimulus-response loops to interact with neural systems and control them in a millisecond time scale. Establishing these loops can be an essential step towards understanding the dynamics of many neural processes and can bridge between traditionally disparate levels of analysis. RT software technology has been previously exploited to build dynamic clamp protocols in electrophysiological preparations . The same principles used in the dynamic-clamp technology can be generalized to develop new techniques of activity-dependent stimulation with applications in a broad spectrum of research in nervous systems . Here we show how RT software technology can also be used to build protocols of activity-dependent real-time drug microinjection to stimulate neural systems.
The heart central pattern generator (CPG) from the cardiac ganglion of the crab Carcinus maenas was subjected to microinjections of GABA. The microinjections were delivered with a Picospritzer III, and the duration of the injection and the stimulation precise instant were controlled. Simultaneously, the membrane potential of one neuron was measured and an activity-dependent stimuli protocol of GABA microinjection was implemented with RT software technology.
We have illustrated a simple protocol of real-time event-driven drug microinjection to achieve a desired state in the spiking-bursting activity of CPG neurons. Real-time software technology allows to implement activity-dependent drug microinjection that can be applied to the study of many aspects of neuromodulation and neurotransmitter stimulation, and to achieve control of pathological states through temporal precise release of drugs.
This work was supported by MEC PHB2007-0013TA, BFU2006-07902/BFI, TIN 2007-65989, CAM S-SEM-0255-2006. RDP was supported by the Brazilian agencies: CAPES, CNPq and FAPESP.
- Prinz AA, Abbott LF, Marder E: The dynamic clamp comes of age. Trends Neurosci. 2004, 27: 218-224. 10.1016/j.tins.2004.02.004.PubMedView ArticleGoogle Scholar
- Muñiz C, Levi R, Benkrid M, Rodríguez FB, Varona P: Real-time control of stepper motors for mechano-sensory stimulation. J Neuroscience Methods. 2008, 172: 105-111. 10.1016/j.jneumeth.2008.04.017.View ArticleGoogle Scholar
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