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Modeling the complete cardiac ganglion – heart muscle network of the crab Callinectes sapidus
BMC Neuroscience volume 10, Article number: P295 (2009)
In the neurogenic heart of the blue crab, Callinectes sapidus , the cardiac ganglion (CG) and the heart muscle form a closed-loop network. Spikes generated by the CG drive contractions of the muscle, which in turn provide feedback to the CG to modify its future spiking. We have modeled the activity of the whole network at the level of the spikes and contractions. At this level, the network can be regarded as composed of three processes (arrows in Fig. 1A): (1) the production of the contractions by the CG spikes, (2) the feedback from the contractions back to the CG, and (3) the endogenous spiking of the CG itself. We first characterized each process separately from experimental data, using a decoding method that we developed for this purpose . For process (1), we obtained three elementary functions to describe the transform from spikes to contractions: K, the single-spike contraction response kernel; H, a history kernel; and F, a static nonlinear function. These three functions were able to successfully predict the contraction response to arbitrary spike trains [3, 4]. For processes (2) and (3), we used a modified version of the same decoding method to find three further elementary functions: H', a history kernel that describes the endogenous spike generation by the CG, that is, the probability of generating a spike given the previous history of spiking; K', a feedback kernel that describes the probability of spiking given the previous history of contraction; and F', a static nonlinear function . We then combined the six elementary functions in a generative model of the complete system implemented in a simulator in MATLAB. The model generates, from the spikes and contractions up to any given time, the next spike and contraction. The computation is purely local in time: no long-range dynamics or oscillatory phenomena such as spike bursts are explicitly represented. Nevertheless, the model, like the real system, produces robust spike bursts and corresponding phasic muscle contractions (Fig. 1B, i). In fact, it does so by two distinct mechanims: the central process (3) and the peripheral feeback loop composed of processes (1) and (2). Either mechanism can generate bursts and phasic contractions alone if the other mechanism is disabled (Figure B, ii, iii). We are now using the simulator to further explore the behavior of the system and to make predictions that will be experimentally testable in the real system.
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Supported by NIH grants NS058017, NS41497, GM08224, and RR03051.