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Database analysis of a computational model of an elemental oscillator in the leech heartbeat neuronal network

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Background

The rhythmic activity of the heartbeat neuronal network of the leech is based on pairs of inhibitory interneurons that make reciprocal spike-mediated and graded synapses across the ganglion midline [1]. In this work, we modeled such a pair of HN(4) reciprocally inhibitory interneurons (Figure 1) with a half-center oscillator (HCO) model. We aim to investigate the changes in this model's oscillatory activity and bursting characteristics based on cellular and synaptic parameters. To achieve this, we varied selected parameters in all combinations by using a brute-force approach and built a database of the resulting model characteristics following earlier work [2, 3].

Figure 1
figure1

Elemental Oscillator Model.

Methods

We represented the individual neurons as single isopotential compartments with Hodgkin and Huxley type intrinsic and synaptic membrane conductances. The model differential equations were integrated by using the exponential Euler method with a time step of 0.1 ms [1]. For our parameter search, we varied eight parameters in both neurons: the maximal conductances of the spike-mediated synapse, graded transmission synapse, and of the persistent Na+, slow Ca2+, leak, hyperpolarization-activated (h), and persistent K+ currents, across of 0, 25, 50, 100, 125, 150, and 175 percent of their canonical values, and the leak reversal potential across -0.07 V, -0.065 V, -0.06 V, -0.055 V, and -0.05 V, resulting in a parameter space of 10,485,760 models. After changing a parameter, a model was run for 100 s to allow the system to establish stable activity, and then, it was run for another 100 s, from which the data were recorded and analyzed. The cycle period was measured as the time between the middle spikes of two consecutive bursts. The HCO model was classified as either bursting, spiking or silent.

Results

We performed all the simulations. However, as a preliminary step in the analysis of the model database, we selected a random subset of 10,000 simulations from the entire set, for which we build a SQL database table [2, 3]. Approximately 37% of these simulations have both cells as being silent, 30% of them have both cells spiking, and 17.5% have both cells bursting with standard alternating HCO activity. The rest of the simulations do not have symmetric activity in the two model cells. We will now use this sample and then the entire database to ask fundamental questions about HCO activity. For example, we will subdivide the bursting HCOs in to those in which the component cells are intrinsically silent, spiking or bursting, and then, ask whether oscillators of these different types respond to parameter changes similarly. We will be particularly interested in parameter changes that correspond to known neuromodulations such as the modulation ofh current by myomodulin [4].

References

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    Hill AAV, Lu J, Masino MA, Olsen OH, Calabrese RL: A model of s segmental oscillator in the leech heartbeat neuronal network. J Comp Neurosci. 2001, 10: 281-302. 10.1023/A:1011216131638.

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    Prinz A, Billimoria CP, Marder E: Alternative to hand-tuning conductance-based models: construction and analysis of databases of model neurons. J Neurophysiol. 2003, 90: 3998-4015. 10.1152/jn.00641.2003.

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    Gunay C, Edgerton JR, Jaeger D: Channel densitydistributions explain spiking variability in the globus pallidus: a combined physiology and computer simulation database approach. J Neurosci. 2008, 28: 7476-7491. 10.1523/JNEUROSCI.4198-07.2008.

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    Tobin AE, Calabrese RL: Myomodulin increases Ih and inhibits the Na/K pump to modulate bursting in leech heart interneurons. J Neurophysiol. 2005, 94: 3938-3950. 10.1152/jn.00340.2005.

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Acknowledgements

This work was supported by the National Institute of Neurological Disorders and Stroke Grant NS024072 to R.L.Calabrese.

Author information

Correspondence to Anca Doloc-Mihu.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Doloc-Mihu, A., Calabrese, R. Database analysis of a computational model of an elemental oscillator in the leech heartbeat neuronal network. BMC Neurosci 10, P263 (2009) doi:10.1186/1471-2202-10-S1-P263

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Keywords

  • Membrane Conductance
  • Inhibitory Interneuron
  • Oscillatory Activity
  • Synaptic Membrane
  • Model Differential Equation