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Modeling the functional connectivity in embodied in vitro neuronal network

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Introduction

We developed a hybrid neuro-robotic bi-directional interface connecting in-vitro neuronal networks to a small mobile robot to investigate neural processes and functional modification that underlie sensorimotor learning in the nervous system [1, 2]. We describe the investigation of the effect of the stimulus-induced distributed plasticity and functional connectivity on the information processing capabilities of the neuronal network. The results evidence a modification at functional connectivity level that can be described in terms of a Hebbian learning rule.

Results

We can model our neuronal network with two input nodes (i.e. feedback stimulation sites) and two output nodes (recoding sites) and we can map functional connectivity among these I/O pathways: the post stimulus time histogram (PSTH) represent the firing probability (of the output node) after a given stimulus (from the input node). Hebb suggested that reverberating activity in assemblies of neurons carries a memory trace that become permanent and it is manifested as changes in synaptic weights between the neurons that persistently take part to firing [3]. A very simple formulaic description of Hebbian learning is:

(1)

Where ω ij is the weight of connection from node j to node i, n is the dimension of the input pattern, p is the number of training patterns and is the k-th input for node i. In our model we have two stimulation sites and two recording sites:

(2)

If we apply it to a limited time window (hundreds of ms) we have applied the formula to compute the Post Stimulus Time Histogram. Thus the description of the functional connectivity in terms of the area of the PSTH can be considered a descriptor to evidence the induced modulations in terms of modulation of hebbian weights and the results from the closed loop session (Figure 1).

Figure 1
figure1

The robot, driven by the network's activity, wandering inside the arena during an experimental session (frame frequency 0.5 Hz).

The hypothetical configuration for the obstacle avoidance achievement requires the cross-connection weights to be weaker than the direct ones. The achieve modulation is close to the theoretical achievement thus confirming the possibility to describe the embodied neuro-robotic paradigm in terms of Hebbian rules.

References

  1. 1.

    Novellino A, D'Angelo P, Cozzi L, Chiappalone M, Sanguineti V, Martinoia S: Connecting neurons to a mobile robot: an in vitro bidirectional neural interface. Comput Intell Neurosci. 2007, 1-13. 10.1155/2007/12725. doi:10.1155/2007/12725.

  2. 2.

    Cozzi L, D'Angelo P, Sanguineti V: Encoding of time-varying stimuli in populations of cultured neurons. Biol Cybern. 2006, 94: 335-349. 10.1007/s00422-006-0051-2. doi: 10.1007/s00422-006-0051-2

  3. 3.

    Hebb DO: Organization of Behavior. 1949, John Wiley & Sons, New York

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Author information

Correspondence to Antonio Novellino.

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Open Access This article is published under license to BioMed Central Ltd. This is an Open Access article is distributed under the terms of the Creative Commons Attribution 2.0 International License (https://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Keywords

  • Functional Connectivity
  • Neuronal Network
  • Obstacle Avoidance
  • Input Node
  • Stimulation Site