Fig. 9

Reduced expression of DA receptor D1 in CPP mice. A Immunofluorescence staining of D1-positive cells in the rACC; scale bars = 100 μm. B Quantitative analysis of D1-positive cells (One-way ANOVA, F_(2,15) = 11.08, P = 0.0011, n = 6 for each group). C Immunofluorescence staining of D2-positive cells in the rACC; scale bars = 50 μm; D Statistical results of D2-positive cells (One-way ANOVA, F_(2,15) = 0.04574, P = 0.9554, n = 6 for each group). E Western blot images were cropped for clarity to display only proteins of interest. The original (cropped) images of full-length blots are in Additional file 2. F Quantitative results of D1 expression levels in each group of mice (One-way ANOVA, F_(2,15) = 5.506, P = 0.0161, n = 6 for each group). G Quantitative results of D2 expression levels in mice of each group (One-way ANOVA, F_(2,15) = 0.1869, P = 0.8314, n = 6 for each group). Data are represented as mean ± SEM, compared with the incision group, #P < 0.05, ##P < 0.01