Fig. 4

Decreased activity of DAergic neurons in CPP mice. A Immunofluorescence staining for TH- and c-Fos-labelled positive cells in VTA of each group, Tyrosine Hydroxylase (TH) in green, c-Fos in red; scale bars = 100 μm. B Quantitative analysis of TH-positive cells (One-way ANOVA, F_(2,15) = 0.04807, P = 0.9532, n = 6 for each group). C Quantification of cells double-labeled for TH and c-Fos (One-way ANOVA, F_(2,15) = 9.390, P = 0.0023, n = 6 for each group). Data are represented as mean ± SEM, compared with the control group; ##P < 0.01