Fig. 10
Optogenetic stimulation of DAergic neurons mitigates nociception response through D1 receptor. A Expression of D1-shRNA virus in the rACC; scale bars = 200 μm. B Statistical results the of D1 mRNA expression levels detected by qPCR (unpaired t-test, P = 0.0004, n = 6 for each group). C Western blot images were edited to exclude non-relevant sections and highlight only target proteins. Original (edited) versions of complete blots are available in Supplementary Material 2. D Quantitative analysis of D1 protein expression in the rACC region (unpaired t-test, P = 0.0006, n = 6 for each group). E PWTs of the left plantar in each group (Two-way RM ANOVA, interaction: F(10;180) = 13.25, P < 0.001, Time: F(10;180) = 212.0, P < 0.001, Group: F(1;180) = 5.655, P < 0.05, n = 10 for each group). F PWTs of the right plantar in each group (Two-way RM ANOVA, interaction: F(10;180) = 0.8397, P > 0.05, Time: F(10;180) = 1.472, P > 0.05, Group: F(1;180) = 0.1134, P > 0.05, n = 10 for each group). Data are represented as mean ± SEM, compared with the control group: ***P < 0.001; compared with CON336 + SI-ChR2 group: *P < 0.05, **P < 0.01