Fig. 6
Spatial expression analysis of Glra3 in the male spinal cord. The spatial Glra3 mRNA expression was examined with RNAscope using probes for Glra3 (teal), Slc17a6 (red) and Slc32a1 (red). Glra3 was expressed in the (A) cervical (C7), (B) thoracic (T11), (C) lumbar (L5) and (D) sacral (S2) divisions of the spinal cord (A–D). Overlap with Slc17a6 and Slc32a1 expressions could be observed in all divisions (a1, a2, b1, b2, c1, c2, d1, d2). Glra3 expression was found in the dorsal and ventral horns in all divisions. Glra3 could be detected in the ventral horn in the cervical, lumbar and sacral divisions. Illustrations of the spinal cord divisions in A–D are modified from https://mouse.brain-map.org/experiment/siv?id=100050402&imageId=101006525&imageType=atlas. The red dashed squares in A–D indicate approximately the area displayed in a1–d2 (labelled with white text). No overview images are shown for the enlarged images labeled with red text. A–D Scale bars: 500 µm, a1–d2: scale bars 100 µm. White arrows denote examples of co-expression. Grey line in a1-d2 indicates boarder for lamina IIouter and lamina IIinner. 5Sp = lamina 5, 6Sp = lamina 6, 7Sp = lamina 7, 8Sp = lamina 8. For all other abbreviations, please see https://mouse.brain-map.org/experiment/siv?id=100050402&imageId=101006525&imageType=atlas