Fig. 3

Analysis of the spatial expression of Glra3 in the female brain. The spatial Glra3 mRNA expression was detected with RNAscope using probes against Glra3 (teal), Slc17a6 (red) and Slc32a1 (red). Expression was detected at (A) Bregma 0.98 mm in the (a1) cortex, and both co-localization of Glra3 with Slc17a6 or Slc32a1 was observed. B At Bregma -1.34 mm, Glra3 expression was found in the (b1) amygdala, (b2) hypothalamus, (b3) thalamus and (b4) hippocampus. b1–b4 Overlap with Slc17a6 expression was observed in all areas, except in the hippocampus, while overlap with Slc32a1 was seen in all areas, except in the thalamus. C At Bregma -6.84 mm, Glra3 expression and overlap with Slc17a6 or Slc32a1 expression was observed in the (c1) brainstem. Illustrations of the Bregma sections in A–C are adapted from https://mouse.brain-map.org/experiment/thumbnails/100048576?image_type=atlas. The red dashed squares in A–C indicate approximately the area displayed in a1–c1. The dashed squares were consistently placed on the right side of the schematic image in order to maximize the readability of the abbreviations, regardless of the position of the representative images. White arrows denote examples of co-expression. Scale bars: 200 µm, enlargements 100 µm. Abbreviated areas in a1–c1: Aco = anterior cortical amygdaloid nucleus, AHP = anterior hypothalamic area, posterior part, BLA = basolateral amygdaloid nucleus, anterior part, BMA = basomedial amygdaloid nucleus, anterior part, DG = dentate gyrus, DM = dorsomedial hypothalamic nucleus, Gi = gigantocellular reticular nucleus, ME = medial amygdaloid nucleus, PLCo = posterolateral cortical amygdaloid nucleus, Rt = reticular thalamic nucleus, S1FL = primary somatosensory cortex, forelimb region, S1J = primary somatosensory cortex, jaw region, Sol = solitary tract, VL = ventrolateral thalamic nucleus, VMH = ventromedial hypothalamic nucleus, VPL = ventral posterolateral thalamic nucleus, VPM = ventral posteromedial thalamic nucleus. For all other abbreviations, please see https://mouse.brain-map.org/experiment/thumbnails/100048576?image_type=atlas