Fig. 1

Low expression of Glra3 was detected in the central nervous system. The expression of Glra3 and its co-expression with excitatory marker Slc17a6 (Vglut2) and inhibitory marker Slc32a1 (Viaat) were examined in distinct areas in the central and peripheral nervous system in the Zeisel et al. (2018) dataset, which contained scRNA-seq data of 27,998 genes in 74,539 neurons [22]. A Dot plot of the expressions of the targeted genes in all neurons in the areas annotated in the Zeisel et al. (2018) dataset. Glra3 was generally expressed in low levels, as indicated by light blue colored dots, and in a small number of cells, as indicated by the dots’ small diameters. The highest expression was found in Zeisel et al. (2018) defined central nervous system areas, namely the amygdala, dorsal midbrain, medulla and the spinal cord (more than 3.0% of neurons in these areas expressed Glra3). B The occurrence of Glra3 neurons (Glra3 was considered expressed if log1p > 0.1) and the co-expression of Slc17a6 and Slc32a1 in the respective areas visualized with a dot plot. Glra3 was expressed in both excitatory and inhibitory neurons, with some areas displaying either excitatory or inhibitory Glra3 neurons, while other areas contained Glra3 neurons of both molecular properties.