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Fig. 2 | BMC Neuroscience

Fig. 2

From: Circ VRK1/microRNA-17/PTEN axis modulates the angiogenesis of human brain microvascular endothelial cells to affect injury induced by oxygen-glucose deprivation/reperfusion

Fig. 2

Silencing of Circ VRK1 reduces apoptosis and promotes viability in OGD/R-treated HBMVECs. HBMVECs were infected with lentiviral vectors harboring Circ VRK1 NC or Circ VRK1 siRNA and exposed to OGD/R. A proliferation of HBMVECs examined by colony formation assay. B apoptosis of HBMVECs examined by flow cytometry. C changes in LDH levels released from HBMVECs examined by cytotoxicity assay. D levels of inflammatory factors in HBMVECs examined by ELISA kits. E oxidative stress factor levels in HBMVECs measured by spectrophotometry and fluorescent probes. Error bars represented the mean ± SD from 3 independent experiments. Unpaired t test (C) and one-way (A, B) or two-way ANOVA (D, E) were used

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BMC Neuroscience

ISSN: 1471-2202

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