Fig. 5

Spectral unmixing of smFISH data in post-mortem human DLPFC brain tissue. Cell nucleus is stained with DAPI, MBP transcripts labeled with Opal520, SLC17A7 transcripts labeled with Opal570, GAD1 transcripts labeled with Opal620, and SNAP25 transcripts labeled with Opal690. Images shown are 2D maximum intensity projections of 3D z-stacks. A–F Linear unmixing results using ZEN software. A′–F′ Spectral unmixing results using FCLSU algorithm. A″–F″ Spectral unmixing results using ELMM algorithm. A‴–F‴ Spectral unmixing results using GELMM algorithm