Fig. 4

Spectral unmixing of smFISH data in a mouse brain tissue section. Nuclei stained with DAPI, Gal transcripts labeled with Opal520, Th transcripts labeled with Opal570, Bdnf exon 9 transcripts labeled with Opal620, and Npy transcripts labeled with Opal690. Images shown are 2D maximum intensity projections of 3D z-stacks. A–E Linear unmixing results using ZEN software. A′–E′ Spectral unmixing results using FCLSU algorithm. A″–E″ Spectral unmixing results using ELMM algorithm. A‴–E‴ Spectral unmixing results using GELMM algorithm