Fig. 11

Biologically relevant changes in hearing thresholds—GMC lines, click and 30 kHz. Volcano plots show significance vs. effect size for all GMC lines. For each mouse line, represented by a dot, hearing thresholds were used to calculate significance (Wilcoxon rank sum test, y-axis) and non-parametric effect size (Cliff’s delta [39], x-axis) of mutant vs. control animals. Vertical lines indicate margins for small (0.147), medium (0.33) and large (0.474) effects as suggested in [38]. An effect size of 1 indicates that the difference between means of mutants and controls is equal to one standard deviation. The horizontal line indicates the 0.05 significance threshold level. Accordingly, dots in the upper left and upper right areas denote GMC lines with significant as well as relevant changes and thus are considered worthwhile candidates (see Additional file 1: Tables S1 and S2). Dot colours in addition represent effect size as shown in the legend. Plot rows represent click (upper) and 30 kHz (lower) stimulus data. Columns compare the three hearing threshold finding methods compared in this work (left: manual, middle: NN, right: SLR)