Fig. 7

Inhibition of NLRP3 inflammasome reversed CIH-related A1/A2 astrocyte phenotypic transformation. a GFAP immunohistochemical staining and GFAP + cell count. b Sholl analysis of morphological changes of astrocytes. c-d Expression of GFAP, C3d, and s100a10 detected by Western blotting and RT-qPCR. Scale bar = 50 μm. Data are presented as the mean ± SD. For RT-qPCR experiments, each group consisted of 4 rats, and the experiment was repeated 3 times; for Western blotting, the experiment was repeated 4 times and there were 5 rats per group. For statistical analysis, an unpaired t-test was used. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001