Fig. 8
From: CaPTure: Calcium PeakToolbox for analysis of in vitro calcium imaging data
Primary mouse cortical neuronal culture data processed through CaPTure: A the raw fluorescent image of mRuby-expressing neurons, its corresponding color-coded neuronal segmentation and the GCaMP6s time-series. B Extracted raw calcium traces, the motif correlation map of the interpolated dff traces and event detection on the interpolated dff traces using the motif correlation maps. Scale bar indicates: 25Â s on x-axis and y-axis is 15000 units of mean fluorescence intensity for raw traces (column 1), 3 units of normalized mean fluorescence intensity for normalized traces (column 3). C Correlation map of inter-neuron calcium activity grouped by the cluster. D Final extracted metrics at image (field) level and ROI (neuron) level