Fig. 2

Extracted calcium traces: the graph shows the calcium activity for each ROI segmented in Step2, and highlights traces 16, 19, and 23 as examples of low-, medium- and high-activity ROIs, respectively. The x-axis is the frame number of the time series and the y-axis range reflects the minimum and maximum fluorescence intensity of the ROIs in this image series (29 and 7387 units of mean fluorescence intensity, respectively, in this example trace). Scale bar indicates: 12 s on x-axis and 5000 units of mean fluorescence intensity on y-axis